N Cadherin (CDH2) (NM_001792) Human Tagged ORF Clone

CAT#: RC207170

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CDH2 (Myc-DDK-tagged)-Human cadherin 2, type 1, N-cadherin (neuronal) (CDH2)



  "NM_001792" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 829.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol CDH2
Synonyms ACOGS; ARVD14; CD325; CDHN; CDw325; NCAD
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC207170 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGCCGGATAGCGGGAGCGCTGCGGACCCTGCTGCCGCTGCTGGCGGCCCTGCTTCAGGCGTCTGTAG
AGGCTTCTGGTGAAATCGCATTATGCAAGACTGGATTTCCTGAAGATGTTTACAGTGCAGTCTTATCGAA
GGATGTGCATGAAGGACAGCCTCTTCTCAATGTGAAGTTTAGCAACTGCAATGGAAAAAGAAAAGTACAA
TATGAGAGCAGTGAGCCTGCAGATTTTAAGGTGGATGAAGATGGCATGGTGTATGCCGTGAGAAGCTTTC
CACTCTCTTCTGAACATGCCAAGTTCCTGATATATGCCCAAGACAAAGAGACCCAGGAAAAGTGGCAAGT
GGCAGTAAAATTGAGCCTGAAGCCAACCTTAACTGAGGAGTCAGTGAAGGAGTCAGCAGAAGTTGAAGAA
ATAGTGTTCCCAAGACAATTCAGTAAGCACAGTGGCCACCTACAAAGGCAGAAGAGAGACTGGGTCATCT
CTCCAATCAACTTGCCAGAAAACTCCAGGGGACCTTTTCCTCAAGAGCTTGTCAGGATCAGGTCTGATAG
AGATAAAAACCTTTCACTGCGGTACAGTGTAACTGGGCCAGGAGCTGACCAGCCTCCAACTGGTATCTTC
ATTATCAACCCCATCTCGGGTCAGCTGTCGGTGACAAAGCCCCTGGATCGCGAGCAGATAGCCCGGTTTC
ATTTGAGGGCACATGCAGTAGATATTAATGGAAATCAAGTGGAGAACCCCATTGACATTGTCATCAATGT
TATTGACATGAATGACAACAGACCTGAGTTCTTACACCAGGTTTGGAATGGGACAGTTCCTGAGGGATCA
AAGCCTGGAACATATGTGATGACCGTAACAGCAATTGATGCTGACGATCCCAATGCCCTCAATGGGATGT
TGAGGTACAGAATCGTGTCTCAGGCTCCAAGCACCCCTTCACCCAACATGTTTACAATCAACAATGAGAC
TGGTGACATCATCACAGTGGCAGCTGGACTTGATCGAGAAAAAGTGCAACAGTATACGTTAATAATTCAA
GCTACAGACATGGAAGGCAATCCCACATATGGCCTTTCAAACACAGCCACGGCCGTCATCACAGTGACAG
ATGTCAATGACAATCCTCCAGAGTTTACTGCCATGACGTTTTATGGTGAAGTTCCTGAGAACAGGGTAGA
CATCATAGTAGCTAATCTAACTGTGACCGATAAGGATCAACCCCATACACCAGCCTGGAACGCAGTGTAC
AGAATCAGTGGCGGAGATCCTACTGGACGGTTCGCCATCCAGACCGACCAAAACAGCAACGACGGGTTAG
TCACCGTGGTCAAACCAATCGACTTTGAAGCAAATAGGATGTTTGTCCTTACTGTTGCTGCAGAAAATCA
AGTGCCATTAGCCAAGGGAATTCAGCACCCGCCTCAGTCAACTGCAACCATGTCTGTTACAGTTATTGAC
GTAAATGAAAACCCTTATTTTGCCCCCAATCCTAAGATCATTCGCCAAGAAGAAGGGCTTCATGCCGGTA
CCATGTTGACAACATTCACTGCTCAGGACCCAGATCGATATATGCAGCAAAATATTAGATACACTAAATT
ATCTGATCCTGCCAATTGGCTAAAAATAGATCCTGTGAATGGACAAATAACTACAATTGCTGTTTTGGAC
CGAGAATCACCAAATGTGAAAAACAATATATATAATGCTACTTTCCTTGCTTCTGACAATGGAATTCCTC
CTATGAGTGGAACAGGAACGCTGCAGATCTATTTACTTGATATTAATGACAATGCCCCTCAAGTGTTACC
TCAAGAGGCAGAGACTTGCGAAACTCCAGACCCCAATTCAATTAATATTACAGCACTTGATTATGGCATT
GATCCAAATGCTGGACCATTTGCTTTTGATCTTCCTTTATCTCCAGTGACTATTAAGAGAAATTGGACCA
TCACTCGGCTTAATGGTGATTTTGCTCAGCTTAATTTAAAGATAAAATTTCTTGAAGCTGGTATCTATGA
AGTTCCCATCATAATCACAGATTCGGGTAATCCTCCCAAATCAAATATTTCCATACTGCGCGTGAAGGTT
TGCCAGTGTGACTCCAACGGGGACTGCACAGATGTGGACAGGATTGTGGGTGCGGGGCTTGGCACCGGTG
CCATCATTGCCATCCTGCTCTGCATCATCATCCTGCTTATCCTTGTGCTGATGTTTGTGGTATGGATGAA
ACGCCGGGATAAAGAACGCCAGGCCAAACAACTTTTAATTGATCCAGAAGATGATGTAAGAGATAATATT
TTAAAATATGATGAAGAAGGTGGAGGAGAAGAAGACCAGGACTATGACTTGAGCCAGCTGCAGCAGCCTG
ACACTGTGGAGCCTGATGCCATCAAGCCTGTGGGAATCCGACGAATGGATGAAAGACCCATCCACGCTGA
GCCCCAGTATCCGGTCCGATCTGCAGCCCCACACCCTGGAGACATTGGGGACTTCATTAATGAGGGCCTT
AAAGCGGCTGACAATGACCCCACAGCTCCACCATATGACTCCCTGTTAGTGTTTGACTATGAAGGCAGTG
GCTCCACTGCTGGGTCCTTGAGCTCCCTTAATTCCTCAAGTAGTGGTGGTGAGCAGGACTATGATTACCT
GAACGACTGGGGGCCACGGTTCAAGAAACTTGCTGACATGTATGGTGGAGGTGATGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC207170 protein sequence
Red=Cloning site Green=Tags(s)

MCRIAGALRTLLPLLAALLQASVEASGEIALCKTGFPEDVYSAVLSKDVHEGQPLLNVKFSNCNGKRKVQ
YESSEPADFKVDEDGMVYAVRSFPLSSEHAKFLIYAQDKETQEKWQVAVKLSLKPTLTEESVKESAEVEE
IVFPRQFSKHSGHLQRQKRDWVISPINLPENSRGPFPQELVRIRSDRDKNLSLRYSVTGPGADQPPTGIF
IINPISGQLSVTKPLDREQIARFHLRAHAVDINGNQVENPIDIVINVIDMNDNRPEFLHQVWNGTVPEGS
KPGTYVMTVTAIDADDPNALNGMLRYRIVSQAPSTPSPNMFTINNETGDIITVAAGLDREKVQQYTLIIQ
ATDMEGNPTYGLSNTATAVITVTDVNDNPPEFTAMTFYGEVPENRVDIIVANLTVTDKDQPHTPAWNAVY
RISGGDPTGRFAIQTDQNSNDGLVTVVKPIDFEANRMFVLTVAAENQVPLAKGIQHPPQSTATMSVTVID
VNENPYFAPNPKIIRQEEGLHAGTMLTTFTAQDPDRYMQQNIRYTKLSDPANWLKIDPVNGQITTIAVLD
RESPNVKNNIYNATFLASDNGIPPMSGTGTLQIYLLDINDNAPQVLPQEAETCETPDPNSINITALDYGI
DPNAGPFAFDLPLSPVTIKRNWTITRLNGDFAQLNLKIKFLEAGIYEVPIIITDSGNPPKSNISILRVKV
CQCDSNGDCTDVDRIVGAGLGTGAIIAILLCIIILLILVLMFVVWMKRRDKERQAKQLLIDPEDDVRDNI
LKYDEEGGGEEDQDYDLSQLQQPDTVEPDAIKPVGIRRMDERPIHAEPQYPVRSAAPHPGDIGDFINEGL
KAADNDPTAPPYDSLLVFDYEGSGSTAGSLSSLNSSSSGGEQDYDYLNDWGPRFKKLADMYGGGDD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001792
ORF Size 2718 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001792.5
RefSeq Size 4380 bp
RefSeq ORF 2721 bp
Locus ID 1000
UniProt ID P19022
Cytogenetics 18q12.1
Domains Cadherin_C_term, CA
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
Protein Pathways Arrhythmogenic right ventricular cardiomyopathy (ARVC), Cell adhesion molecules (CAMs)
MW 99.8 kDa
Gene Summary This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone. [provided by RefSeq, Nov 2015]

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