Cryptochrome I (CRY1) (NM_004075) Human Tagged ORF Clone

CAT#: RC207152

CRY1 (Myc-DDK-tagged)-Human cryptochrome 1 (photolyase-like) (CRY1)

Product Images

Circular map for RC207152

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CRY1 (Cat# RC207152, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRY1 (Cat# TA809887)(1:2000). Positive lysates LY418241 (100ug) and LC418241 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY418241) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207152 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CRY1 protein (Cat# TP307152). The protein was produced from HEK293T cells transfected with CRY1 cDNA clone (Cat# RC207152) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cryptochrome I
• Synonyms: DSPD; PHLL1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207152 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGTGAACGCCGTGCACTGGTTCCGAAAGGGGCTCCGGCTCCACGACAACCCCGCCCTGAAGGAGT
  GCATTCAGGGCGCCGACACCATCCGCTGCGTCTACATCCTGGACCCCTGGTTCGCCGGCTCCTCCAATGT
  GGGCATCAACAGGTGGCGATTTTTGCTTCAGTGTCTTGAGGATCTTGATGCCAATCTACGAAAATTAAAC
  TCCCGTCTGTTTGTGATTCGTGGACAACCAGCAGATGTGTTTCCCAGGCTTTTCAAGGAATGGAACATTA
  CTAAACTTTCAATTGAGTATGATTCTGAGCCCTTTGGAAAGGAACGAGACGCAGCTATTAAGAAACTGGC
  AACTGAAGCTGGAGTAGAAGTCATTGTAAGAATTTCACATACATTATATGACCTAGACAAGATCATAGAA
  CTCAATGGTGGACAACCGCCTCTAACTTATAAAAGATTCCAGACTCTCATCAGCAAAATGGAACCACTAG
  AGATACCAGTAGAGACAATTACTTCAGAAGTGATAGAAAAGTGCACAACTCCTCTGTCTGATGACCATGA
  TGAGAAATATGGAGTCCCTTCACTGGAAGAGCTAGGTTTTGATACAGATGGCTTATCCTCTGCAGTGTGG
  CCAGGCGGAGAAACTGAAGCACTTACTCGTTTGGAAAGGCATTTGGAAAGAAAAGCTTGGGTGGCAAATT
  TTGAAAGACCTCGAATGAATGCGAATTCTCTGCTTGCAAGCCCTACTGGACTTAGTCCTTATCTCCGATT
  TGGTTGTTTGTCATGTCGACTGTTTTACTTCAAACTAACAGATCTCTACAAAAAGGTAAAGAAGAACAGT
  TCCCCTCCCCTTTCCCTTTATGGGCAACTGTTATGGCGTGAATTTTTCTATACAGCAGCAACAAATAATC
  CACGCTTTGATAAAATGGAAGGAAACCCTATCTGTGTTCAGATTCCTTGGGATAAAAATCCTGAGGCTTT
  AGCCAAATGGGCGGAAGGCCGGACAGGCTTTCCATGGATTGATGCCATCATGACACAGCTTCGTCAGGAG
  GGTTGGATTCATCATCTAGCCAGGCATGCAGTTGCTTGCTTCCTGACACGAGGGGACCTGTGGATTAGTT
  GGGAAGAAGGAATGAAGGTATTTGAAGAATTATTGCTTGATGCAGATTGGAGCATAAATGCTGGAAGTTG
  GATGTGGCTGTCTTGTAGTTCCTTTTTTCAACAGTTTTTTCACTGCTATTGCCCTGTTGGTTTTGGTAGG
  AGAACAGATCCCAATGGAGACTATATCAGGCGTTATTTGCCTGTCCTAAGAGGCTTCCCTGCAAAATATA
  TCTATGATCCCTGGAATGCACCAGAAGGTATCCAAAAGGTAGCCAAATGTTTGATAGGAGTTAATTATCC
  TAAACCAATGGTGAACCATGCTGAGGCAAGCCGTTTGAATATCGAAAGGATGAAACAGATCTATCAGCAG
  CTTTCACGATATAGAGGACTAGGTCTTCTGGCATCAGTACCTTCTAATCCTAATGGGAATGGAGGCTTCA
  TGGGATATTCTGCAGAAAATATCCCAGGTTGTAGCAGCAGTGGAAGTTGCTCTCAAGGGAGTGGTATTTT
  ACACTATGCTCATGGCGACAGTCAGCAAACTCACCTGTTGAAGCAAGGAAGAAGCTCCATGGGCACTGGT
  CTCAGTGGTGGGAAACGTCCTAGTCAGGAAGAGGACACACAGAGTATTGGTCCTAAAGTCCAGAGACAGA
  GCACTAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207152 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGVNAVHWFRKGLRLHDNPALKECIQGADTIRCVYILDPWFAGSSNVGINRWRFLLQCLEDLDANLRKLN
  SRLFVIRGQPADVFPRLFKEWNITKLSIEYDSEPFGKERDAAIKKLATEAGVEVIVRISHTLYDLDKIIE
  LNGGQPPLTYKRFQTLISKMEPLEIPVETITSEVIEKCTTPLSDDHDEKYGVPSLEELGFDTDGLSSAVW
  PGGETEALTRLERHLERKAWVANFERPRMNANSLLASPTGLSPYLRFGCLSCRLFYFKLTDLYKKVKKNS
  SPPLSLYGQLLWREFFYTAATNNPRFDKMEGNPICVQIPWDKNPEALAKWAEGRTGFPWIDAIMTQLRQE
  GWIHHLARHAVACFLTRGDLWISWEEGMKVFEELLLDADWSINAGSWMWLSCSSFFQQFFHCYCPVGFGR
  RTDPNGDYIRRYLPVLRGFPAKYIYDPWNAPEGIQKVAKCLIGVNYPKPMVNHAEASRLNIERMKQIYQQ
  LSRYRGLGLLASVPSNPNGNGGFMGYSAENIPGCSSSGSCSQGSGILHYAHGDSQQTHLLKQGRSSMGTG
  LSGGKRPSQEEDTQSIGPKVQRQSTN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004075
• ORF Size: 1758 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004075.5
• RefSeq Size: 3310 bp
• RefSeq ORF: 1761 bp
• Locus ID: 1407
• UniProt ID: Q16526
• Cytogenetics: 12q23.3
• Domains: FAD_binding_7, DNA_photolyase
• Protein Families: Druggable Genome
• Protein Pathways: Circadian rhythm - mammal
• MW: 66.4 kDa
• Gene Summary: This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. Loss of the related gene in mouse results in a shortened circadian cycle in complete darkness. [provided by RefSeq, Jan 2014]