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MINDY1 (NM_001040217) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC207090
FAM63A (Myc-DDK-tagged)-Human family with sequence similarity 63, member A (FAM63A), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $503.00
3 Weeks*
Size
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

TA335967 Rabbit Polyclonal Anti-FAM63A Antibody 100 ul
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TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags 30 ul
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CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA) 5 x 200 ul
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PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each 100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug 10 ug
$650.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL 5 ml
$720.00

Other products for "MINDY1 or NM_001040217"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MINDY1
Synonyms FAM63A; MINDY-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207090 representing NM_001040217
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAATACCATCAGCCTGAGGATCCAGCCCCTGGTAAGGCCGGGACTGCAGAAGCAGTCATCCCTGAAA
ACCATGAGGTTCTGGCAGGCCCAGATGAGCACCCTCAGGACACAGATGCAAGAGATGCTGATGGGGAGGC
TAGAGAACGGGAGCCAGCAGACCAAGCTTTGCTGCCTAGCCAGTGTGGGGACAACCTTGAGTCCCCTCTG
CCTGAAGCTAGCTCAGCTCCACCGGGGCCAACCCTTGGGACACTGCCTGAAGTAGAGACAATAAGGGCAT
GCTCCATGCCCCAGGAGCTTCCTCAGTCCCCCAGGACCCGACAGCCTGAGCCAGATTTCTACTGTGTCAA
GTGGATCCCTTGGAAAGGAGAACAGACACCCATCATCACCCAGAGCACTAACGGCCCTTGCCCTCTCCTT
GCCATCATGAACATCCTCTTTCTTCAGTGGAAGGTGAAGCTCCCCCCGCAGAAGGAAGTGATCACATCGG
ATGAGCTCATGGCCCATCTTGGAAACTGCCTCCTGTCCATCAAGCCCCAGGAGAAGTCAGAGGGACTTCA
GCTTAATTTTCAGCAGAATGTGGATGATGCAATGACAGTGCTGCCTAAACTGGCCACAGGTCTGGATGTC
AATGTGCGATTCACAGGCGTCTCTGATTTTGAGTATACACCCGAGTGCAGTGTCTTTGACCTGCTAGGCA
TACCTCTGTACCATGGCTGGCTTGTTGATCCACAGAGTCCTGAGGCTGTGCGTGCAGTTGGGAAACTGAG
TTACAACCAGCTGGTGGAGAGGATCATCACCTGCAAACACTCCAGTGACACCAACCTCGTGACAGAAGGC
CTGATTGCAGAGCAGTTCCTGGAGACCACCGCGGCCCAGCTGACCTACCACGGACTGTGTGAGCTGACAG
CAGCTGCTAAGGAGGGTGAACTTAGCGTCTTTTTCCGAAACAACCACTTTAGCACCATGACTAAGCATAA
GAGTCACTTATACCTACTGGTCACTGACCAGGGCTTTCTACAGGAGGAGCAAGTCGTATGGGAGAGCCTG
CACAATGTGGATGGAGACAGCTGCTTTTGTGACTCTGACTTTCACCTGAGTCATTCCCTGGGCAAGGGGC
CTGGAGCAGAAGGTGGGAGTGGCTCCCCAGAAAAGCAGCTGCAGGTAGACCAGGACTACCTGATTGCTCT
GTCCCTGCAGCAGCAACAGCCACGAGGCCCGCTGGGGCTTACCGACTTGGAGCTGGCCCAGCAGCTTCAG
CAAGAGGAGTATCAACAGCAGCAGGCAGCGCAGCCAGTGCGGATGCGGACGCGGGTCCTGTCACTGCAGG
GGAGAGGAGCCACATCTGGACGCCCAGCCGGGGAGCGTCGGCAGAGGCCGAAGCACGAGTCAGACTGCAT
TCTGCTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC207090 representing NM_001040217
Red=Cloning site Green=Tags(s)

MEYHQPEDPAPGKAGTAEAVIPENHEVLAGPDEHPQDTDARDADGEAREREPADQALLPSQCGDNLESPL
PEASSAPPGPTLGTLPEVETIRACSMPQELPQSPRTRQPEPDFYCVKWIPWKGEQTPIITQSTNGPCPLL
AIMNILFLQWKVKLPPQKEVITSDELMAHLGNCLLSIKPQEKSEGLQLNFQQNVDDAMTVLPKLATGLDV
NVRFTGVSDFEYTPECSVFDLLGIPLYHGWLVDPQSPEAVRAVGKLSYNQLVERIITCKHSSDTNLVTEG
LIAEQFLETTAAQLTYHGLCELTAAAKEGELSVFFRNNHFSTMTKHKSHLYLLVTDQGFLQEEQVVWESL
HNVDGDSCFCDSDFHLSHSLGKGPGAEGGSGSPEKQLQVDQDYLIALSLQQQQPRGPLGLTDLELAQQLQ
QEEYQQQQAAQPVRMRTRVLSLQGRGATSGRPAGERRQRPKHESDCILL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001040217
ORF Size 1407 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001040217.1, NM_001040217.2, NP_001035307.1
RefSeq Size 2187 bp
RefSeq ORF 984 bp
Locus ID 55793
UniProt ID Q8N5J2
Cytogenetics 1q21.3
MW 51.8 kDa
Summary Hydrolase that can specifically remove 'Lys-48'-linked conjugated ubiquitin from proteins. Has exodeubiquitinase activity and has a preference for long polyubiquitin chains. May play a regulatory role at the level of protein turnover.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001040217" in other vectors (4)
SKU Description Size Price
RC207090L3 Lenti-ORF clone of FAM63A (Myc-DDK-tagged)-Human family with sequence similarity 63, member A (FAM63A), transcript variant 2 10 ug
$803.00
RC207090L4 Lenti-ORF clone of FAM63A (mGFP-tagged)-Human family with sequence similarity 63, member A (FAM63A), transcript variant 2 10 ug
$803.00
RG207090 FAM63A (tGFP-tagged) - Human family with sequence similarity 63, member A (FAM63A), transcript variant 2 10 ug
$703.00
SC322576 FAM63A (untagged)-Human family with sequence similarity 63, member A (FAM63A), transcript variant 2 10 ug
$668.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.