MYO1F (NM_012335) Human Tagged Lenti ORF Clone

  • LentiORF®
SKU
RC207069L1
Lenti ORF clone of Human myosin IF (MYO1F), Myc-DDK-tagged
  $1,733.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MYO1F
Vector pLenti-C-Myc-DDK
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection None
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC207069).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_012335
ORF Size 3294 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012335.2
RefSeq Size 3860 bp
RefSeq ORF 3297 bp
Locus ID 4542
UniProt ID O00160
Cytogenetics 19p13.2
Domains IQ, myosin_head, SH3
MW 124.7 kDa
Summary Myosins are molecular motors that use the energy from ATP hydrolysis to generate force on actin filaments. The protein encoded by this gene is an unconventional myosin that may be involved in the intracellular movement of membrane-enclosed compartments. There is evidence to suggest that mutations in this gene can result in hearing loss. provided by RefSeq, Jan 2017
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Documents
Resources
"NM_012335" in other vectors (6)
SKU Description Size Price
RC207069 MYO1F (Myc-DDK-tagged)-Human myosin IF (MYO1F) 10 ug
$1,433.00
RC207069L2 Lenti ORF clone of Human myosin IF (MYO1F), mGFP tagged 10 ug
$1,733.00
RC207069L3 Lenti ORF clone of Human myosin IF (MYO1F), Myc-DDK-tagged 10 ug
$1,733.00
RC207069L4 Lenti ORF clone of Human myosin IF (MYO1F), mGFP tagged 10 ug
$1,733.00
RG207069 MYO1F (tGFP-tagged) - Human myosin IF (MYO1F) 10 ug
$1,633.00
SC101088 MYO1F (untagged)-Human myosin IF (MYO1F) 10 ug
$1,435.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.