TYSND1 (NM_001040273) Human Tagged ORF Clone

CAT#: RC207043

TYSND1 (Myc-DDK-tagged)-Human trypsin domain containing 1 (TYSND1), transcript variant 2

Product Images

Circular map for RC207043

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TYSND1
• Synonyms: NET41
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207043 representing NM_001040273
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAAGGCAGTGGGGGTCTGCCATGAGGGCGGCCGAGCAGGCGGGCTGCATGGTGAGCGCCTCCCGGG
  CCGGACAGCCCGAGGCGGGCCCGTGGAGCTGCAGCGGGGTAATCCTGAGCCGTAGCCCGGGCCTGGTGCT
  TTGCCACGGGGGCATCTTCGTCCCCTTCCTGCGAGCTGGCAGCGAAGTCCTGACCGCGGCCGGCGCCGTC
  TTCCTGCCTGGCGACAGTTGCAGGGACGACCTGCGCCTGCACGTGCAGTGGGCCCCAACGGCCGCGGGTC
  CCGGGGGCGGCGCGGAGCGGGGCCGCCCAGGGCTGTGCACGCCCCAGTGCGCCAGCCTCGAGCCCGGCCC
  ACCTGCCCCGTCCCGCGGGCGTCCCCTGCAGCCCCGGCTTCCTGCTGAGCTGCTGCTGCTGCTGAGCTGC
  CCGGCCTTCTGGGCCCACTTCGCGCGCCTCTTCGGGGACGAGGCAGCGGAACAGTGGCGCTTCTCGAGCG
  CGGCGCGGGATGACGAAGTGTCGGAGGACGAGGAGGCGGATCAACTGAGAGCGCTGGGCTGGTTTGCGCT
  GCTGGGCGTGCGGCTAGGCCAGGAGGAGGTGGAGGAGGAGCGCGGGCTAGCCATGGCGGTGTCGCCTCTC
  GGGGCCGTGCCCAAGGGCGCGCCATTGCTGGTCTGCGGCTCCCCTTTCGGCGCCTTCTGCCCCGACATCT
  TTCTCAACACGCTGAGCTGCGGGGTGCTCAGCAACGTGGCCGGCCCACTGCTGCTTACCGACGCACGCTG
  CCTGCCCGGCACCGAGGGCGGCGGCGTGTTCACCGCGCGGCCCGCGGGGGCGCTGGTGGCGCTGGTGGTG
  GCGCCGCTCTGTTGGAAGGCCGGCGAATGGGTGGGCTTCACGCTGCTCTGCGCCGCCGCCCCCCTTTTCC
  GCGCCGCCCGCGACGCGCTTCACCGCCTGCCGCACAGCACCGCTGCCCTGGCCGCCCTTCTGCCGCCAGA
  GGTGGGCGTCCCGTGGGGTCTGCCCCTCCGAGACTCCGGGCCCCTGTGGGCAGCCGCGGCAGTGTTGGTG
  GAGTGCGGCACCGTATGGGGCTCCGGAGTGGCTGTGGCACCCCGCCTTGTAGTGACCTGTCGGCACGTGT
  CCCCTCGGGAAGCAGCCAGGGTCCTGGTGCGCTCCACCACCCCCAAGCATAATCACCAGCAACACCCGGG
  ACAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207043 representing NM_001040273
  Red=Cloning site Green=Tags(s)
  
  MRRQWGSAMRAAEQAGCMVSASRAGQPEAGPWSCSGVILSRSPGLVLCHGGIFVPFLRAGSEVLTAAGAV
  FLPGDSCRDDLRLHVQWAPTAAGPGGGAERGRPGLCTPQCASLEPGPPAPSRGRPLQPRLPAELLLLLSC
  PAFWAHFARLFGDEAAEQWRFSSAARDDEVSEDEEADQLRALGWFALLGVRLGQEEVEEERGLAMAVSPL
  GAVPKGAPLLVCGSPFGAFCPDIFLNTLSCGVLSNVAGPLLLTDARCLPGTEGGGVFTARPAGALVALVV
  APLCWKAGEWVGFTLLCAAAPLFRAARDALHRLPHSTAALAALLPPEVGVPWGLPLRDSGPLWAAAAVLV
  ECGTVWGSGVAVAPRLVVTCRHVSPREAARVLVRSTTPKHNHQQHPGQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001040273
• ORF Size: 1194 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001040273.3
• RefSeq Size: 3485 bp
• RefSeq ORF: 1197 bp
• Locus ID: 219743
• UniProt ID: Q2T9J0
• Cytogenetics: 10q22.1
• Protein Families: Druggable Genome
• MW: 41.4 kDa
• Gene Summary: This gene encodes a protease that removes the N-terminal peroxisomal targeting signal (PTS2) from proteins produced in the cytosol, thereby facilitating their import into the peroxisome. The encoded protein is also capable of removing the C-terminal peroxisomal targeting signal (PTS1) from proteins in the peroxisomal matrix. The full-length protein undergoes self-cleavage to produce shorter, potentially inactive, peptides. Alternative splicing results in multiple transcript variants for this gene. [provided by RefSeq, Jan 2013]