CNPase (CNP) (NM_033133) Human Tagged ORF Clone

CAT#: RC207038

CNP (Myc-DDK-tagged)-Human 2',3'-cyclic nucleotide 3' phosphodiesterase (CNP)

Product Images

Circular map for RC207038

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CNP (Cat# RC207038, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CNP (1:2000) (Cat# TA811100). Positive lysates LY409699 (100ug) and LC409699 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY409699) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207038 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CNP protein (Cat# TP307038). The protein was produced from HEK293T cells transfected with CNP cDNA clone (Cat# RC207038) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CNPase
• Synonyms: CNP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207038 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAGAGGCTTCTCCCGAAAAAGCCACACATTCCTGCCCAAGATCTTCTTCCGCAAGATGTCATCCT
  CAGGGGCCAAGGACAAGCCTGAGCTGCAGTTTCCCTTCCTTCAGGATGAGGACACAGTGGCCACGCTGCT
  AGAGTGCAAGACGCTCTTCATCTTGCGCGGCCTGCCAGGAAGCGGCAAGTCCACGCTGGCACGGGTCATC
  GTGGACAAGTACCGTGATGGCACCAAGATGGTGTCGGCTGACGCTTACAAGATCACCCCCGGCGCTCGAG
  GAGCCTTCTCCGAGGAGTACAAGCGGCTCGATGAGGACCTGGCTGCCTACTGCCGCCGCCGGGACATCAG
  AATTCTTGTGCTTGATGACACCAACCACGAACGGGAACGGCTGGAGCAGCTCTTTGAAATGGCCGACCAG
  TACCAGTACCAGGTGGTGCTGGTGGAGCCCAAGACGGCGTGGCGGCTGGACTGTGCCCAGCTCAAGGAGA
  AGAACCAGTGGCAGCTGTCGGCTGATGACCTGAAGAAGCTGAAGCCTGGGCTGGAGAAGGACTTCCTGCC
  GCTCTACTTCGGCTGGTTCCTGACCAAGAAGAGCTCTGAGACCCTCCGCAAAGCCGGCCAGGTCTTCCTG
  GAAGAGCTGGGGAACCACAAGGCCTTCAAGAAGGAGCTGCGACAATTCGTCCCTGGGGATGAGCCCAGGG
  AGAAGATGGACTTGGTCACCTACTTTGGAAAGAGACCCCCAGGCGTGCTGCATTGCACAACCAAGTTTTG
  TGACTACGGGAAGGCTCCCGGGGCAGAGGAGTACGCTCAACAAGATGTGTTAAAGAAATCTTACTCCAAG
  GCCTTCACGCTGACCATCTCTGCCCTCTTTGTGACACCCAAGACGACTGGGGCCCGGGTGGAGTTAAGCG
  AGCAGCAACTGCAGTTGTGGCCGAGTGATGTGGACAAGCTGTCACCCACTGACAACCTGCCGCGGGGGAG
  CCGCGCCCACATCACCCTCGGCTGTGCAGCTGACGTAGAGGCCGTGCAGACGGGCCTTGACCTCTTAGAG
  ATTCTGCGGCAGGAGAAGGGGGGCAGCCGAGGCGAGGAGGTGGGCGAGCTAAGCCGGGGCAAGCTCTATT
  CCTTGGGCAATGGGCGCTGGATGCTGACCCTGGCCAAGAACATGGAGGTCAGGGCCATCTTCACGGGGTA
  CTACGGGAAAGGCAAACCTGTGCCCACGCAAGGTAGCCGGAAGGGGGGCGCCTTGCAGTCCTGCACCATC
  ATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207038 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNRGFSRKSHTFLPKIFFRKMSSSGAKDKPELQFPFLQDEDTVATLLECKTLFILRGLPGSGKSTLARVI
  VDKYRDGTKMVSADAYKITPGARGAFSEEYKRLDEDLAAYCRRRDIRILVLDDTNHERERLEQLFEMADQ
  YQYQVVLVEPKTAWRLDCAQLKEKNQWQLSADDLKKLKPGLEKDFLPLYFGWFLTKKSSETLRKAGQVFL
  EELGNHKAFKKELRQFVPGDEPREKMDLVTYFGKRPPGVLHCTTKFCDYGKAPGAEEYAQQDVLKKSYSK
  AFTLTISALFVTPKTTGARVELSEQQLQLWPSDVDKLSPTDNLPRGSRAHITLGCAADVEAVQTGLDLLE
  ILRQEKGGSRGEEVGELSRGKLYSLGNGRWMLTLAKNMEVRAIFTGYYGKGKPVPTQGSRKGGALQSCTI
  I
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033133
• ORF Size: 1263 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033133.5
• RefSeq Size: 5222 bp
• RefSeq ORF: 1266 bp
• Locus ID: 1267
• UniProt ID: P09543
• Cytogenetics: 17q21.2
• MW: 47.6 kDa
• Gene Summary: May participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin.[UniProtKB/Swiss-Prot Function]