Alpha SNAP (NAPA) (NM_003827) Human Tagged ORF Clone

CAT#: RC207037

NAPA (Myc-DDK-tagged)-Human N-ethylmaleimide-sensitive factor attachment protein, alpha (NAPA)

Product Images

Circular map for RC207037

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  Western blot validation of overexpression lysate (Cat# LY401256) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207037 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified NAPA protein (Cat# TP307037). The protein was produced from HEK293T cells transfected with NAPA cDNA clone (Cat# RC207037) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Alpha SNAP
• Synonyms: SNAPA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207037 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAATTCCGGGAAGGAAGCGGAGGCGATGGCGCTGTTGGCCGAGGCGGAGCGCAAAGTGAAGAACT
  CGCAGTCCTTCTTCTCTGGCCTCTTTGGAGGCTCATCCAAAATAGAGGAAGCATGCGAAATCTACGCCAG
  AGCAGCAAACATGTTCAAAATGGCCAAAAACTGGAGTGCTGCTGGAAACGCGTTCTGCCAGGCTGCACAG
  CTGCACCTGCAGCTCCAGAGCAAGCACGACGCAGCCACCTGCTTTGTGGACGCTGGCAACGCATTCAAGA
  AAGCCGACCCCCAAGAGGCCATTAACTGTTTGATGCGAGCAATCGAGATCTACACAGACATGGGCCGATT
  CACGATTGCGGCCAAGCACCACATCTCCATTGCTGAGATCTATGAGACAGAGTTGGTGGACATCGAGAAG
  GCCATTGCCCACTACGAGCAGTCTGCAGACTACTACAAAGGCGAGGAGTCCAACAGCTCAGCCAACAAGT
  GTCTGCTGAAGGTGGCTGGTTACGCTGCGCTGCTGGAGCAGTATCAGAAGGCCATTGACATCTACGAACA
  GGTGGGGACCAATGCCATGGACAGCCCCCTCCTCAAGTACAGCGCCAAAGACTACTTCTTCAAGGCGGCC
  CTCTGCCACTTCTGCATCGACATGCTCAACGCCAAGCTGGCTGTCCAAAAGTATGAGGAGCTGTTCCCAG
  CTTTCTCTGATTCCCGGGAATGCAAGTTGATGAAAAAATTGCTAGAGGCCCACGAGGAGCAGAATGTGGA
  CAGCTACACCGAGTCGGTGAAGGAATACGACTCCATCTCCCGGCTGGACCAGTGGCTCACCACCATGCTG
  CTGCGCATCAAGAAGACCATCCAGGGCGATGAGGAGGACCTGCGC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207037 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDNSGKEAEAMALLAEAERKVKNSQSFFSGLFGGSSKIEEACEIYARAANMFKMAKNWSAAGNAFCQAAQ
  LHLQLQSKHDAATCFVDAGNAFKKADPQEAINCLMRAIEIYTDMGRFTIAAKHHISIAEIYETELVDIEK
  AIAHYEQSADYYKGEESNSSANKCLLKVAGYAALLEQYQKAIDIYEQVGTNAMDSPLLKYSAKDYFFKAA
  LCHFCIDMLNAKLAVQKYEELFPAFSDSRECKLMKKLLEAHEEQNVDSYTESVKEYDSISRLDQWLTTML
  LRIKKTIQGDEEDLR
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003827
• ORF Size: 885 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003827.4
• RefSeq Size: 1865 bp
• RefSeq ORF: 888 bp
• Locus ID: 8775
• UniProt ID: P54920
• Cytogenetics: 19q13.32-q13.33
• Domains: NSF
• MW: 33.2 kDa
• Gene Summary: This gene encodes a member of the soluble NSF attachment protein (SNAP) family. SNAP proteins play a critical role in the docking and fusion of vesicles to target membranes as part of the 20S NSF-SNAP-SNARE complex. The encoded protein plays a role in the completion of membrane fusion by mediating the interaction of N-ethylmaleimide-sensitive factor (NSF) with the vesicle-associated and membrane-associated SNAP receptor (SNARE) complex, and stimulating the ATPase activity of NSF. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jun 2011]