IL10RA (NM_001558) Human Tagged ORF Clone

CAT#: RC207020

IL10RA (Myc-DDK-tagged)-Human interleukin 10 receptor, alpha (IL10RA), transcript variant 1

Product Images

Circular map for RC207020

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY IL10RA (Cat# RC207020, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL10RA(Cat# TA506929). Positive lysates LY400596 (100ug) and LC400596 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY400596) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207020 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified IL10RA protein (Cat# TP307020). The protein was produced from HEK293T cells transfected with IL10RA cDNA clone (Cat# RC207020) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IL10RA
• Synonyms: CD210; CD210a; CDW210A; HIL-10R; IL-10R1; IL10R
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207020 representing NM_001558
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCCGTGCCTCGTAGTGCTGCTGGCGGCGCTCCTCAGCCTCCGTCTTGGCTCAGACGCTCATGGGA
  CAGAGCTGCCCAGCCCTCCGTCTGTGTGGTTTGAAGCAGAATTTTTCCACCACATCCTCCACTGGACACC
  CATCCCAAATCAGTCTGAAAGTACCTGCTATGAAGTGGCACTCCTGAGGTATGGAATAGAGTCCTGGAAC
  TCCATCTCCAACTGTAGCCAGACCCTGTCCTATGACCTTACCGCAGTGACCTTGGACCTGTACCACAGCA
  ATGGCTACCGGGCCAGAGTGCGGGCTGTGGACGGCAGCCGGCACTCCAACTGGACCGTCACCAACACCCG
  CTTCTCTGTGGATGAAGTGACTCTGACAGTTGGCAGTGTGAACCTAGAGATCCACAATGGCTTCATCCTC
  GGGAAGATTCAGCTACCCAGGCCCAAGATGGCCCCCGCAAATGACACATATGAAAGCATCTTCAGTCACT
  TCCGAGAGTATGAGATTGCCATTCGCAAGGTGCCGGGAAACTTCACGTTCACACACAAGAAAGTAAAACA
  TGAAAACTTCAGCCTCCTAACCTCTGGAGAAGTGGGAGAGTTCTGTGTCCAGGTGAAACCATCTGTCGCT
  TCCCGAAGTAACAAGGGGATGTGGTCTAAAGAGGAGTGCATCTCCCTCACCAGGCAGTATTTCACCGTGA
  CCAACGTCATCATCTTCTTTGCCTTTGTCCTGCTGCTCTCCGGAGCCCTCGCCTACTGCCTGGCCCTCCA
  GCTGTATGTGCGGCGCCGAAAGAAGCTACCCAGTGTCCTGCTCTTCAAGAAGCCCAGCCCCTTCATCTTC
  ATCAGCCAGCGTCCCTCCCCAGAGACCCAAGACACCATCCACCCGCTTGATGAGGAGGCCTTTTTGAAGG
  TGTCCCCAGAGCTGAAGAACTTGGACCTGCACGGCAGCACAGACAGTGGCTTTGGCAGCACCAAGCCATC
  CCTGCAGACTGAAGAGCCCCAGTTCCTCCTCCCTGACCCTCACCCCCAGGCTGACAGAACGCTGGGAAAC
  GGGGAGCCCCCTGTGCTGGGGGACAGCTGCAGTAGTGGCAGCAGCAATAGCACAGACAGCGGGATCTGCC
  TGCAGGAGCCCAGCCTGAGCCCCAGCACAGGGCCCACCTGGGAGCAACAGGTGGGGAGCAACAGCAGGGG
  CCAGGATGACAGTGGCATTGACTTAGTTCAAAACTCTGAGGGCCGGGCTGGGGACACACAGGGTGGCTCG
  GCCTTGGGCCACCACAGTCCCCCGGAGCCTGAGGTGCCTGGGGAAGAAGACCCAGCTGCTGTGGCATTCC
  AGGGTTACCTGAGGCAGACCAGATGTGCTGAAGAGAAGGCAACCAAGACAGGCTGCCTGGAGGAAGAATC
  GCCCTTGACAGATGGCCTTGGCCCCAAATTCGGGAGATGCCTGGTTGATGAGGCAGGCTTGCATCCACCA
  GCCCTGGCCAAGGGCTATTTGAAACAGGATCCTCTAGAAATGACTCTGGCTTCCTCAGGGGCCCCAACGG
  GACAGTGGAACCAGCCCACTGAGGAATGGTCACTCCTGGCCTTGAGCAGCTGCAGTGACCTGGGAATATC
  TGACTGGAGCTTTGCCCATGACCTTGCCCCTCTAGGCTGTGTGGCAGCCCCAGGTGGTCTCCTGGGCAGC
  TTTAACTCAGACCTGGTCACCCTGCCCCTCATCTCTAGCCTGCAGTCAAGTGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207020 representing NM_001558
  Red=Cloning site Green=Tags(s)
  
  MLPCLVVLLAALLSLRLGSDAHGTELPSPPSVWFEAEFFHHILHWTPIPNQSESTCYEVALLRYGIESWN
  SISNCSQTLSYDLTAVTLDLYHSNGYRARVRAVDGSRHSNWTVTNTRFSVDEVTLTVGSVNLEIHNGFIL
  GKIQLPRPKMAPANDTYESIFSHFREYEIAIRKVPGNFTFTHKKVKHENFSLLTSGEVGEFCVQVKPSVA
  SRSNKGMWSKEECISLTRQYFTVTNVIIFFAFVLLLSGALAYCLALQLYVRRRKKLPSVLLFKKPSPFIF
  ISQRPSPETQDTIHPLDEEAFLKVSPELKNLDLHGSTDSGFGSTKPSLQTEEPQFLLPDPHPQADRTLGN
  GEPPVLGDSCSSGSSNSTDSGICLQEPSLSPSTGPTWEQQVGSNSRGQDDSGIDLVQNSEGRAGDTQGGS
  ALGHHSPPEPEVPGEEDPAAVAFQGYLRQTRCAEEKATKTGCLEEESPLTDGLGPKFGRCLVDEAGLHPP
  ALAKGYLKQDPLEMTLASSGAPTGQWNQPTEEWSLLALSSCSDLGISDWSFAHDLAPLGCVAAPGGLLGS
  FNSDLVTLPLISSLQSSE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001558
• ORF Size: 1734 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001558.4
• RefSeq Size: 3649 bp
• RefSeq ORF: 1737 bp
• Locus ID: 3587
• UniProt ID: Q13651
• Cytogenetics: 11q23.3
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway
• MW: 62.9 kDa
• Gene Summary: The protein encoded by this gene is a receptor for interleukin 10. This protein is structurally related to interferon receptors. It has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene. [provided by RefSeq, Jan 2009]