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p107 (RBL1) (NM_183404) Human Tagged Lenti ORF Clone

  • LentiORF®
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SKU
RC207017L2
Lenti ORF clone of Human retinoblastoma-like 1 (p107) (RBL1), transcript variant 2, mGFP tagged
ORF Plasmid:
mGFP DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $1,183.00
3 Weeks*
Size
Bulk Requests & Clone Modifications

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Other products for "p107 or NM_183404"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol p107
Synonyms CP107; p107; PRB1
Vector pLenti-C-mGFP
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection None
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC207017).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_183404
ORF Size 3042 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_183404.1
RefSeq Size 3305 bp
RefSeq ORF 3045 bp
Locus ID 5933
UniProt ID P28749
Cytogenetics 20q11.23
Protein Families Druggable Genome, Transcription Factors
Protein Pathways Cell cycle, TGF-beta signaling pathway
MW 114.7 kDa
Summary The protein encoded by this gene is similar in sequence and possibly function to the product of the retinoblastoma 1 (RB1) gene. The RB1 gene product is a tumor suppressor protein that appears to be involved in cell cycle regulation, as it is phosphorylated in the S to M phase transition and is dephosphorylated in the G1 phase of the cell cycle. Both the RB1 protein and the product of this gene can form a complex with adenovirus E1A protein and SV40 large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. In addition, both proteins can inhibit the transcription of cell cycle genes containing E2F binding sites in their promoters. Due to the sequence and biochemical similarities with the RB1 protein, it is thought that the protein encoded by this gene may also be a tumor suppressor. Two transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_183404" in other vectors (6)
SKU Description Size Price
RC207017 RBL1 (Myc-DDK-tagged)-Human retinoblastoma-like 1 (p107) (RBL1), transcript variant 2 10 ug
$883.00
RC207017L1 Lenti ORF clone of Human retinoblastoma-like 1 (p107) (RBL1), transcript variant 2, Myc-DDK-tagged 10 ug
$1,183.00
RC207017L3 Lenti ORF clone of Human retinoblastoma-like 1 (p107) (RBL1), transcript variant 2, Myc-DDK-tagged 10 ug
$1,183.00
RC207017L4 Lenti ORF clone of Human retinoblastoma-like 1 (p107) (RBL1), transcript variant 2, mGFP tagged 10 ug
$1,183.00
RG207017 RBL1 (tGFP-tagged) - Human retinoblastoma-like 1 (p107) (RBL1), transcript variant 2 10 ug
$789.00 $1,083.00
SC109715 RBL1 (untagged)-Human retinoblastoma-like 1 (p107) (RBL1), transcript variant 2 10 ug
$884.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.