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SPO11 (NM_198265) Human Tagged ORF Clone

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SKU
RC206998
SPO11 (Myc-DDK-tagged)-Human SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae) (SPO11), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Size
Bulk Requests & Clone Modifications

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Other products for "SPO11 or NM_198265"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SPO11
Synonyms CT35; SPATA43; TOPOVIA; TOPVIA
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206998 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTTTGCACCTATGGGGCCCGAGGCCTCGTTCTTCGACGTTTTGGACCGACACAGGGAGTCCCTGC
TGGCTGCCCTGAGGAGAGGTGGCAGGGAGCCCCCAACTGGGGGAAGCCGCCTGGCCTCCAGGTTTGAAGA
TTCTGTGGGTCTTCAGATGGTATCCCATTGCACCACCAGAAAGATCAAAAGTGATTCACCAAAATCAGCT
CAAAAATTTTCTCTAATCCTTAAAATATTGTCCATGATTTATAAATTAGTACAGAGCAACACTTATGCAA
CCAAAAGGGACATATATTACACTGACAGTCAACTCTTTGGTAACCAGACTGTCGTCGACAATATTATCAA
TGACATTTCTTGCATGTTAAAAGTGTCAAGGAGGAGTCTACATATATTATCTACATCAAAAGGTTTAATT
GCTGGCAACTTAAGATACATCGAGGAAGATGGCACCAAAGTGAATTGTACCTGTGGTGCAACGGCTGTTG
CTGTGCCATCGAATATTCAAGGAATTCGGAATTTAGTTACAGATGCAAAGTTTGTATTAATTGTAGAAAA
AGATGCAACATTTCAGCGGCTCCTAGATGACAACTTTTGCAACAAATTGTCTCCTTGCATCATGATTACG
GGAAAGGGAGTTCCTGATCTAAACACAAGACTTTTAGTCAAGAAACTGTGGGATACATTTCATGTTCCTG
TTTTCACTCTTGTAGATGCTGATCCACATGGCATAGAAATAATGTGCATCTATAAGTATGGATCTATGTC
TATGTCTTTTGAAGCTCATCATCTCACAGTTCCAGCTATTAGATGGCTTGGTCTTCTCCCTTCTGATCTT
AAAAGATTAAATGTACCTAAAGATAGTTTGATTCCACTGACAAAAAGGGACCAAATGAAACTTGACAGTA
TCCTGAGGAGACCTTATGTTACCTGCCAACCATTTTGGAGAAAAGAAATGGAAATAATGGCAGACTCTAA
AATGAAGGCAGAAATTCAAGCTTTGACTTTCCTATCATCAGATTATCTTTCCAGAGTGTACTTACCTAAC
AAATTAAAATTTGGAGGATGGATA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC206998 protein sequence
Red=Cloning site Green=Tags(s)

MAFAPMGPEASFFDVLDRHRESLLAALRRGGREPPTGGSRLASRFEDSVGLQMVSHCTTRKIKSDSPKSA
QKFSLILKILSMIYKLVQSNTYATKRDIYYTDSQLFGNQTVVDNIINDISCMLKVSRRSLHILSTSKGLI
AGNLRYIEEDGTKVNCTCGATAVAVPSNIQGIRNLVTDAKFVLIVEKDATFQRLLDDNFCNKLSPCIMIT
GKGVPDLNTRLLVKKLWDTFHVPVFTLVDADPHGIEIMCIYKYGSMSMSFEAHHLTVPAIRWLGLLPSDL
KRLNVPKDSLIPLTKRDQMKLDSILRRPYVTCQPFWRKEMEIMADSKMKAEIQALTFLSSDYLSRVYLPN
KLKFGGWI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_198265
ORF Size 1074 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_198265.2
RefSeq Size 1712 bp
RefSeq ORF 1077 bp
Locus ID 23626
UniProt ID Q9Y5K1
Cytogenetics 20q13.31
Protein Families Druggable Genome, Transcription Factors
MW 40.4 kDa
Summary Meiotic recombination and chromosome segregation require the formation of double-strand breaks (DSBs) in paired chromosome homologs. During meiosis in yeast, a meiotic recombination protein is covalently-linked to the 5' end of DSBs and is essential for the formation of DSBs. The protein encoded by this gene is similar in sequence and conserved features to the yeast meiotic recombination protein. The encoded protein belongs to the TOP6A protein family. Several transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two of them have been described. [provided by RefSeq, Jul 2008]
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Documents
Resources
"NM_198265" in other vectors (4)
SKU Description Size Price
RC206998L3 Lenti ORF clone of Human SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae) (SPO11), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC206998L4 Lenti ORF clone of Human SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae) (SPO11), transcript variant 2, mGFP tagged 10 ug
$757.00
RG206998 SPO11 (tGFP-tagged) - Human SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae) (SPO11), transcript variant 2 10 ug
$657.00
SC125697 SPO11 (untagged)-Human SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae) (SPO11), transcript variant 2 10 ug
$457.00

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