TMEM63A (NM_014698) Human Tagged ORF Clone

CAT#: RC206992

TMEM63A (Myc-DDK-tagged)-Human transmembrane protein 63A (TMEM63A)

Product Images

Circular map for RC206992

• 

  Western blot validation of overexpression lysate (Cat# LY415076) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206992 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TMEM63A
• Synonyms: HLD19; KIAA0792
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206992 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGACTCCCCGTTCCTGGAGCTGTGGCAGTCCAAGGCAGTGTCCATCAGGGAGCAGCTGGGACTCG
  GGGACCGGCCCAACGACTCCTATTGCTACAACTCGGCCAAAAACAGCACCGTGCTCCAGGGGGTCACCTT
  TGGTGGCATCCCCACTGTCCTGCTCATAGACGTCAGCTGCTTCCTGTTCTTAATCTTGGTGTTTTCTATT
  ATAAGAAGAAGATTCTGGGACTATGGCCGCATTGCCCTGGTGTCAGAAGCAGACAGCGAGTCCAGATTTC
  AGAGATTGTCATCGACTTCCTCCTCAGGTCAACAAGACTTTGAAAATGAGCTGGGATGCTGTCCCTGGCT
  GACTGCCATCTTCCGTCTGCATGATGACCAGATCCTGGAATGGTGTGGGGAGGACGCCATCCACTACCTG
  TCCTTCCAGAGGCACATCATCTTCCTGTTGGTGGTGGTCAGCTTTTTGTCCCTGTGTGTCATCCTGCCTG
  TCAACCTCTCAGGGGACTTGCTGGACAAAGACCCGTATAGTTTTGGGAGGACAACAATAGCAAACCTACA
  GACTGACAATGACCTCCTTTGGCTGCACACCATCTTTGCTGTCATTTACCTCTTCCTCACTGTGGGTTTC
  ATGCGGCACCACACTCAGTCCATTAAGTACAAAGAGGAGAACCTGGTGAGGCGGACCCTGTTCATCACAG
  GACTCCCCAGAGATGCCAGGAAGGAGACTGTGGAGAGCCACTTCCGGGACGCGTATCCCACGTGTGAGGT
  GGTTGATGTGCAGCTGTGCTACAACGTGGCCAAACTGATCTACCTGTGCAAGGAGAAAAAGAAGACTGAG
  AAGAGCCTGACCTATTACACAAACCTGCAGGTGAAGACAGGCCAGCGGACCCTCATCAACCCCAAGCCCT
  GTGGCCAGTTTTGCTGCTGTGAAGTGCTGGGCTGTGAGTGGGAAGACGCCATCTCTTACTACACACGGAT
  GAAGGACAGGCTGCTGGAGAGGATCACAGAGGAAGAACGCCACGTCCAGGACCAGCCCCTGGGAATGGCC
  TTCGTCACCTTCCAGGAGAAGTCCATGGCCACCTACATCCTGAAAGATTTCAATGCCTGCAAGTGTCAGA
  GCCTTCAGTGCAAAGGTGAGCCCCAGCCGTCCTCCCATAGCAGGGAGCTCTATACCTCCAAGTGGACAGT
  CACCTTTGCTGCTGACCCTGAGGACATCTGCTGGAAGAACCTCTCTATCCAGGGCCTCCGCTGGTGGCTA
  CAGTGGCTGGGCATCAACTTCACCCTCTTCCTGGGGCTATTTTTCCTGACCACACCCTCCATCATCCTGT
  CCACCATGGACAAGTTTAATGTCACCAAACCCATCCATGCGCTGAATAACCCGATCATCAGCCAGTTCTT
  CCCCACCCTCCTGCTCTGGTCCTTCTCGGCCCTGCTCCCCTCCATTGTCTACTACTCTACACTGCTGGAG
  TCTCACTGGACCAAGTCGGGGGAAAACCAGATCATGATGACCAAAGTCTACATATTCTTGATCTTCATGG
  TGCTGATCCTGCCCTCCCTGGGTCTCACCAGTCTAGATTTTTTCTTCCGGTGGCTCTTTGACAAAACTTC
  CTCGGAGGCCTCCATCAGGTTGGAGTGCGTCTTCCTGCCTGACCAGGGTGCCTTCTTTGTGAACTATGTC
  ATCGCCTCGGCCTTCATCGGCAATGGCATGGAGCTGCTGCGGCTGCCAGGTCTCATCCTCTATACCTTCC
  GCATGATCATGGCCAAGACGGCTGCTGACCGCAGGAATGTCAAGCAGAACCAGGCCTTCCAGTACGAGTT
  TGGAGCCATGTATGCATGGATGCTGTGTGTCTTCACTGTCATCGTGGCCTACAGCATCACTTGTCCCATC
  ATCGCGCCATTTGGCCTCATCTACATCCTGCTCAAGCACATGGTGGACCGGCACAACCTCTACTTCGTCT
  ACCTCCCAGCCAAGCTGGAGAAGGGGATCCACTTTGCCGCTGTGAACCAGGCCTTGGCAGCCCCCATCCT
  GTGCCTCTTCTGGCTCTACTTCTTTTCCTTCCTGCGCCTGGGTATGAAGGCCCCCGCCACTCTGTTCACC
  TTCCTGGTGCTGCTGCTCACCATCCTGGTCTGCCTGGCTCACACCTGCTTTGGATGCTTCAAGCACCTCA
  GCCCTCTGAACTACAAAACAGAAGAGCCTGCAAGTGACAAAGGAAGTGAGGCAGAGGCCCACATGCCCCC
  ACCGTTCACACCCTACGTGCCTCGGATTCTGAACGGCTTGGCCTCGGAGAGGACAGCACTGTCTCCGCAG
  CAGCAGCAGCAGCAGACCTATGGTGCCATCCACAACATCAGCGGGACTATCCCTGGACAGTGCTTGGCGC
  AGAGCGCCACGGGCAGTGTGGCTGCTGCCCCCCAGGAGGCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206992 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MMDSPFLELWQSKAVSIREQLGLGDRPNDSYCYNSAKNSTVLQGVTFGGIPTVLLIDVSCFLFLILVFSI
  IRRRFWDYGRIALVSEADSESRFQRLSSTSSSGQQDFENELGCCPWLTAIFRLHDDQILEWCGEDAIHYL
  SFQRHIIFLLVVVSFLSLCVILPVNLSGDLLDKDPYSFGRTTIANLQTDNDLLWLHTIFAVIYLFLTVGF
  MRHHTQSIKYKEENLVRRTLFITGLPRDARKETVESHFRDAYPTCEVVDVQLCYNVAKLIYLCKEKKKTE
  KSLTYYTNLQVKTGQRTLINPKPCGQFCCCEVLGCEWEDAISYYTRMKDRLLERITEEERHVQDQPLGMA
  FVTFQEKSMATYILKDFNACKCQSLQCKGEPQPSSHSRELYTSKWTVTFAADPEDICWKNLSIQGLRWWL
  QWLGINFTLFLGLFFLTTPSIILSTMDKFNVTKPIHALNNPIISQFFPTLLLWSFSALLPSIVYYSTLLE
  SHWTKSGENQIMMTKVYIFLIFMVLILPSLGLTSLDFFFRWLFDKTSSEASIRLECVFLPDQGAFFVNYV
  IASAFIGNGMELLRLPGLILYTFRMIMAKTAADRRNVKQNQAFQYEFGAMYAWMLCVFTVIVAYSITCPI
  IAPFGLIYILLKHMVDRHNLYFVYLPAKLEKGIHFAAVNQALAAPILCLFWLYFFSFLRLGMKAPATLFT
  FLVLLLTILVCLAHTCFGCFKHLSPLNYKTEEPASDKGSEAEAHMPPPFTPYVPRILNGLASERTALSPQ
  QQQQQTYGAIHNISGTIPGQCLAQSATGSVAAAPQEA
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014698
• ORF Size: 2421 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014698.3
• RefSeq Size: 4469 bp
• RefSeq ORF: 2424 bp
• Locus ID: 9725
• UniProt ID: O94886
• Cytogenetics: 1q42.12
• Domains: DUF221
• Protein Families: Transmembrane
• MW: 92.1 kDa
• Gene Summary: Acts as an osmosensitive calcium-permeable cation channel.[UniProtKB/Swiss-Prot Function]