BANK1 (NM_017935) Human Tagged ORF Clone

CAT#: RC206985

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  • TrueORF®

BANK1 (Myc-DDK-tagged)-Human B-cell scaffold protein with ankyrin repeats 1 (BANK1), transcript variant 1



  "NM_017935" in other vectors (6)

USD 420.00


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Size
    • 10 ug


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Specifications

Product Data
Product Name BANK1 (NM_017935) Human Tagged ORF Clone
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol BANK1
Synonyms BANK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>RC206985 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCCAGCAGCGCCAGGCAAGGGGCTTGGGAGCCCGGACCCCGCCCCCTGCGGCCCAGCGCCCCCAG
GAAATACAAAAGATATAATAATGATATATGAAGAAGATGCTGAGGAATGGGCTCTGTACTTGACAGAAGT
ATTTTTACATGTTGTGAAAAGGGAAGCCATCCTGTTATATCGCTTGGAGAATTTCTCTTTTCGGCATTTG
GAGTTGCTGAACTTAACGTCTTACAAATGTAAACTTTTGATATTATCAAATAGCCTGCTTAGAGACCTAA
CTCCAAAGAAATGTCAGTTTCTGGAAAAGATACTTCATTCACCAAAAAGTGTAGTTACTTTGCTTTGTGG
AGTGAAGAGTTCAGATCAGCTCTATGAATTACTAAATATCTCTCAAAGCAGATGGGAGATCTCAACTGAA
CAGGAACCTGAAGACTACATCTCTGTAATCCAGAGTATCATATTCAAAGATTCTGAAGACTACTTTGAGG
TCAACATTCCAACAGACCTACGAGCAAAACATTCTGGGGAAATAAGTGAGAGAAAGGAAATTGAAGAACT
ATCAGAAGCTTCAAGAAACACCATACCACTAGCAGTGGTGCTTCCCACTGAAATTCCATGTGAGAATCCT
GGTGAAATATTCATAATTTTGAGAGATGAAGTAATTGGTGATACTGTAGAGGTTGAATTTACATCAAGTA
ATAAGCGCATTAGAACACGGCCAGCCCTTTGGAATAAGAAAGTCTGGTGCATGAAAGCTTTAGAGTTTCC
TGCTGGTTCAGTCCATGTCAATGTCTACTGTGATGGAATCGTTAAAGCTACAACCAAAATTAAGTACTAC
CCAACAGCAAAGGCAAAGGAATGCCTATTCAGAATGGCAGATTCAGGAGAGAGTTTGTGCCAGAATAGCA
TTGAAGAACTTGATGGTGTCCTTACATCCATATTCAAACATGAGATACCATATTATGAGTTCCAGTCTCT
TCAAACTGAAATTTGTTCTCAAAACAAATATACTCATTTCAAAGAACTTCCAACTCTTCTCCACTGTGCA
GCAAAATTTGGCTTAAAGAACCTGGCTATTCATTTGCTTCAATGTTCAGGAGCAACCTGGGCATCTAAGA
TGAAAAATATGGAGGGTTCAGACCCCGCACATATTGCTGAAAGGCATGGTCACAAAGAACTCAAGAAAAT
CTTCGAAGACTTTTCAATCCAAGAAATTGACATAAATAATGAGCAAGAAAATGATTATGAAGAGGATATT
GCCTCATTTTCCACATATATTCCTTCCACACAGAACCCAGCATTTCATCATGAAAGCAGAAAGACATACG
GGCAGAGTGCAGATGGAGCTGAGGCAAATGAAATGGAAGGGGAAGGAAAACAGAATGGATCAGGCATGGA
GACCAAACACAGCCCACTAGAGGTTGGCAGTGAGAGTTCTGAAGACCAGTATGATGACTTGTATGTGTTC
ATTCCTGGTGCTGATCCAGAAAATAATTCACAAGAGCCACTCATGAGCAGCAGACCTCCTCTCCCCCCGC
CGCGACCTGTAGCTAATGCCTTCCAACTGGAAAGACCTCACTTCACCTTACCAGGGACAATGGTGGAAGG
CCAAATGGAAAGAAGTCAAAACTGGGGTCATCCTGGTGTTAGACAAGAAACAGGAGATGAACCCAAAGGA
GAAAAAGAGAAGAAAGAAGAGGAAAAAGAGCAGGAGGAGGAAGAAGACCCATATACTTTTGCTGAGATTG
ATGACAGTGAATATGACATGATATTGGCCAATCTGAGTATAAAGAAAAAAACTGGGAGTCGGTCTTTCAT
TATAAATAGACCTCCTGCCCCCACACCCCGACCCACAAGTATACCTCCAAAAGAGGAAACTACACCTTAC
ATAGCTCAAGTGTTTCAACAAAAGACAGCCAGAAGACAATCTGATGATGACAAGTTCCGTGGTCTTCCTA
AGAAACAAGACAGAGCTCGGATAGAGAGTCCAGCCTTTTCTACTCTCAGGGGCTGTCTAACTGATGGTCA
GGAAGAACTCATCCTCCTGCAGGAGAAAGTAAAGAATGGGAAAATGTCTATGGATGAAGCTCTGGAGAAA
TTTAAACACTGGCAGATGGGAAAAAGTGGCCTGGAAATGATTCAGCAGGAGAAATTACGACAACTACGAG
ACTGCATTATTGGGAAAAGGCCAGAAGAAGAAAATGTCTATAATAAACTCACCATTGTGCACCATCCAGG
TGGTAAGGAAACTGCCCACAATGAAAATAAGTTTTATAATGTACACTTCAGCAATAAGCTTCCTGCTCGA
CCCCAAGTTGAAAAGGAATTTGGTTTCTGTTGCAAGAAAGATCAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC206985 protein sequence
Red=Cloning site Green=Tags(s)

MLPAAPGKGLGSPDPAPCGPAPPGNTKDIIMIYEEDAEEWALYLTEVFLHVVKREAILLYRLENFSFRHL
ELLNLTSYKCKLLILSNSLLRDLTPKKCQFLEKILHSPKSVVTLLCGVKSSDQLYELLNISQSRWEISTE
QEPEDYISVIQSIIFKDSEDYFEVNIPTDLRAKHSGEISERKEIEELSEASRNTIPLAVVLPTEIPCENP
GEIFIILRDEVIGDTVEVEFTSSNKRIRTRPALWNKKVWCMKALEFPAGSVHVNVYCDGIVKATTKIKYY
PTAKAKECLFRMADSGESLCQNSIEELDGVLTSIFKHEIPYYEFQSLQTEICSQNKYTHFKELPTLLHCA
AKFGLKNLAIHLLQCSGATWASKMKNMEGSDPAHIAERHGHKELKKIFEDFSIQEIDINNEQENDYEEDI
ASFSTYIPSTQNPAFHHESRKTYGQSADGAEANEMEGEGKQNGSGMETKHSPLEVGSESSEDQYDDLYVF
IPGADPENNSQEPLMSSRPPLPPPRPVANAFQLERPHFTLPGTMVEGQMERSQNWGHPGVRQETGDEPKG
EKEKKEEEKEQEEEEDPYTFAEIDDSEYDMILANLSIKKKTGSRSFIINRPPAPTPRPTSIPPKEETTPY
IAQVFQQKTARRQSDDDKFRGLPKKQDRARIESPAFSTLRGCLTDGQEELILLQEKVKNGKMSMDEALEK
FKHWQMGKSGLEMIQQEKLRQLRDCIIGKRPEEENVYNKLTIVHHPGGKETAHNENKFYNVHFSNKLPAR
PQVEKEFGFCCKKDH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_017935
ORF Size 2355 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_017935.2, NM_017935.3, NM_017935.4, NP_060405.2
RefSeq Size 3455 bp
RefSeq ORF 2358 bp
Locus ID 55024
Cytogenetics 4q24
MW 89.3 kDa
Gene Summary The protein encoded by this gene is a B-cell-specific scaffold protein that functions in B-cell receptor-induced calcium mobilization from intracellular stores. This protein can also promote Lyn-mediated tyrosine phosphorylation of inositol 1,4,5-trisphosphate receptors. Polymorphisms in this gene are associated with susceptibility to systemic lupus erythematosus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

Citations (1)

Other Versions

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