INPP5E (NM_019892) Human Tagged ORF Clone

CAT#: RC206984

INPP5E (Myc-DDK-tagged)-Human inositol polyphosphate-5-phosphatase, 72 kDa (INPP5E)

Product Images

Circular map for RC206984

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  Western blot validation of overexpression lysate (Cat# LY412676) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206984 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified INPP5E protein (Cat# TP306984). The protein was produced from HEK293T cells transfected with INPP5E cDNA clone (Cat# RC206984) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: INPP5E
• Synonyms: CORS1; CPD4; JBTS1; MORMS; pharbin; PPI5PIV
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206984 representing NM_019892
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGTCCAAGGCGGAGAATCTGCGGCCCTCCGAGCCGGCCCCGCAGCCGCCGGAAGGGAGGACGCTCC
  AAGGACAGCTTCCCGGCGCTCCGCCGGCCCAGCGCGCGGGGTCCCCACCCGATGCTCCGGGCTCCGAGAG
  CCCCGCGCTTGCCTGCAGCACTCCGGCCACGCCCAGCGGCGAGGACCCGCCAGCCCGAGCAGCACCCATC
  GCCCCGCGGCCCCCCGCCAGGCCTCGACTGGAGCGAGCCCTGTCCCTGGACGACAAGGGCTGGAGGAGGA
  GGCGTTTTCGAGGCAGCCAGGAGGACCTGGAAGCCCGGAATGGGACCAGTCCCTCCAGGGGCTCAGTGCA
  GAGCGAGGGCCCCGGGGCCCCCGCCCACAGCTGCTCCCCGCCCTGCCTGAGCACCTCCTTGCAGGAAATC
  CCCAAGTCCCGCGGGGTCCTGAGCAGTGAGAGAGGGAGCCCGTCCTCGGGGGGTAACCCTCTCTCTGGGG
  TGGCCAGCAGCTCCCCGAACCTCCCGCACAGAGACGCCGCCGTGGCGGGGAGCTCGCCCAGGCTGCCCAG
  CCTGCTGCCCCCGCGCCCACCGCCTGCCCTGAGCCTGGACATCGCCTCCGACTCCCTGAGGACAGCAAAC
  AAGGTCGACTCGGATCTTGCAGACTACAAGCTCCGCGCGCAGCCGCTCCTGGTGCGGGCCCACAGCAGCC
  TGGGCCCCGGCCGGCCGCGGAGCCCCCTGGCCTGCGACGACTGTTCCCTTCGCTCGGCCAAATCCTCCTT
  CAGCCTCCTGGCGCCCATCCGCAGCAAGGACGTCCGCAGCAGGAGTTACCTGGAGGGCAGCCTCCTGGCC
  AGCGGGGCCCTGTTGGGGGCGGATGAGCTGGCCCGCTACTTCCCAGACCGGAACGTGGCACTCTTCGTGG
  CCACCTGGAACATGCAGGGCCAGAAGGAGCTCCCGCCCAGCCTGGACGAGTTCCTGCTCCCAGCCGAGGC
  CGACTATGCCCAGGACCTGTATGTCATCGGGGTCCAGGAGGGCTGTTCTGACAGGCGGGAGTGGGAGACT
  CGTCTGCAGGAGACGCTGGGCCCGCACTATGTGCTGCTGTCCTCGGCGGCCCACGGCGTGCTCTACATGT
  CGCTCTTCATCCGCAGGGACCTCATCTGGTTCTGCTCAGAGGTGGAGTGCTCCACGGTGACCACACGCAT
  CGTGTCTCAGATCAAGACCAAGGGGGCCTTGGGCATCAGCTTCACCTTTTTTGGCACTTCCTTCCTCTTC
  ATCACGTCCCACTTCACCTCAGGTGACGGGAAGGTGGCGGAGCGGCTGCTGGACTACACCAGGACTGTAC
  AAGCCCTGGTCCTGCCCAGAAATGTGCCCGACACCAACCCCTATCGCTCCAGCGCAGCGGACGTCACCAC
  CCGCTTCGATGAGGTGTTCTGGTTTGGAGACTTCAACTTCCGCCTGAGTGGCGGGCGCACAGTCGTGGAC
  GCCCTCCTGTGCCAGGGCCTGGTGGTGGACGTGCCGGCGCTGCTGCAGCACGACCAGCTCATCCGGGAGA
  TGCGGAAAGGGTCCATCTTCAAGGGCTTCCAGGAGCCGGACATCCACTTCCTCCCATCATACAAGTTTGA
  CATCGGGAAGGACACGTACGACAGCACCTCCAAGCAGAGGACGCCCTCATACACGGACCGCGTCTTGTAC
  AGAAGCCGCCACAAGGGTGACATCTGTCCTGTGAGCTACTCTTCCTGCCCCGGGATCAAGACGTCCGACC
  ACCGCCCTGTGTATGGCCTCTTCCGGGTGAAAGTGAGGCCGGGGCGAGACAACATTCCGTTGGCAGCTGG
  CAAATTTGATAGAGAACTGTACTTACTAGGAATTAAAAGACGGATTTCGAAGGAGATTCAGAGGCAGCAA
  GCACTACAGAGTCAGAACTCCAGCACCATCTGCTCCGTTTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206984 representing NM_019892
  Red=Cloning site Green=Tags(s)
  
  MPSKAENLRPSEPAPQPPEGRTLQGQLPGAPPAQRAGSPPDAPGSESPALACSTPATPSGEDPPARAAPI
  APRPPARPRLERALSLDDKGWRRRRFRGSQEDLEARNGTSPSRGSVQSEGPGAPAHSCSPPCLSTSLQEI
  PKSRGVLSSERGSPSSGGNPLSGVASSSPNLPHRDAAVAGSSPRLPSLLPPRPPPALSLDIASDSLRTAN
  KVDSDLADYKLRAQPLLVRAHSSLGPGRPRSPLACDDCSLRSAKSSFSLLAPIRSKDVRSRSYLEGSLLA
  SGALLGADELARYFPDRNVALFVATWNMQGQKELPPSLDEFLLPAEADYAQDLYVIGVQEGCSDRREWET
  RLQETLGPHYVLLSSAAHGVLYMSLFIRRDLIWFCSEVECSTVTTRIVSQIKTKGALGISFTFFGTSFLF
  ITSHFTSGDGKVAERLLDYTRTVQALVLPRNVPDTNPYRSSAADVTTRFDEVFWFGDFNFRLSGGRTVVD
  ALLCQGLVVDVPALLQHDQLIREMRKGSIFKGFQEPDIHFLPSYKFDIGKDTYDSTSKQRTPSYTDRVLY
  RSRHKGDICPVSYSSCPGIKTSDHRPVYGLFRVKVRPGRDNIPLAAGKFDRELYLLGIKRRISKEIQRQQ
  ALQSQNSSTICSVS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019892
• ORF Size: 1932 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019892.6
• RefSeq Size: 3440 bp
• RefSeq ORF: 1935 bp
• Locus ID: 56623
• UniProt ID: Q9NRR6
• Cytogenetics: 9q34.3
• Protein Families: Druggable Genome
• Protein Pathways: Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• MW: 70 kDa
• Gene Summary: The protein encoded by this gene is an inositol 1,4,5-trisphosphate (InsP3) 5-phosphatase. InsP3 5-phosphatases hydrolyze Ins(1,4,5)P3, which mobilizes intracellular calcium and acts as a second messenger mediating cell responses to various stimulation. Studies of the mouse counterpart suggest that this protein may hydrolyze phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,5-bisphosphate on the cytoplasmic Golgi membrane and thereby regulate Golgi-vesicular trafficking. Mutations in this gene cause Joubert syndrome; a clinically and genetically heterogenous group of disorders characterized by midbrain-hindbrain malformation and various associated ciliopathies that include retinal dystrophy, nephronophthisis, liver fibrosis and polydactyly. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]