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TORC1 (CRTC1) (NM_015321) Human Tagged ORF Clone

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SKU
RC206974
CRTC1 (Myc-DDK-tagged)-Human CREB regulated transcription coactivator 1 (CRTC1), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $590.00
In Stock*
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Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TORC1
Synonyms Mam-2; MAML2; MECT1; TORC-1; TORC1; WAMTP1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206974 representing NM_015321
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGACTTCGAACAATCCGCGGAAATTCAGCGAGAAGATCGCGCTGCACAATCAGAAGCAGGCGGAGG
AGACGGCGGCCTTCGAGGAGGTCATGAAGGACCTGAGCCTGACGCGGGCCGCGCGGCTCCAGCTCCAGAA
ATCCCAGTACCTGCAACTGGGCCCCAGCCGAGGCCAGTACTATGGCGGGTCCCTGCCCAACGTGAACCAG
ATCGGGAGTGGCACCATGGACCTGCCCTTCCAGACCCCCTTCCAATCCTCGGGCCTGGACACCAGCCGGA
CCACCCGGCACCATGGGCTGGTGGACAGGGTGTACCGGGAGCGTGGCCGGCTCGGCTCCCCACACCGCCG
GCCCCTGTCAGTGGACAAACACGGACGGCAGGCCGACAGCTGCCCCTATGGCACCATGTACCTCTCACCA
CCCGCGGACACCAGCTGGAGAAGGACCAATTCTGACTCCGCCCTGCACCAGAGCACAATGACGCCCACGC
AGCCAGAATCCTTTAGCAGTGGGTCCCAGGACGTGCACCAGAAAAGAGTCTTACTGTTAACAGTCCCAGG
AATGGAAGAGACCACATCAGAGGCAGACAAAAACCTTTCCAAGCAAGCATGGGACACCAAGAAGACGGGG
TCCAGGCCCAAGTCCTGTGAGGTCCCCGGAATCAACATCTTCCCGTCTGCCGACCAGGAAAACACTACAG
CCCTGATCCCCGCCACCCACAACACAGGGGGGTCCCTGCCCGACCTGACCAACATCCACTTCCCCTCCCC
GCTCCCGACCCCGCTGGACCCCGAGGAGCCCACCTTCCCTGCACTGAGCAGCTCCAGCAGCACCGGCAAC
CTCGCGGCCAACCTGACGCACCTGGGCATCGGTGGCGCCGGCCAGGGAATGAGCACACCTGGCTCCTCTC
CACAGCACCGCCCAGCTGGCGTCAGCCCCCTGTCCCTGAGCACAGAGGCAAGGCGTCAGCAGGCATCGCC
CGCCCTGTCCCCGCTGTCACCCATCACTCAGGCTGTAGCCATGGACGCCCTGTCTCTGGAGCAGCAGCTG
CCCTACGCCTTCTTCACCCAGGCGGGCTCCCAGCAGCCACCGCCGCAGCCCCAGCCCCCGCCGCCTCCTC
CACCCGCGTCCCAGCAGCCACCACCCCCGCCACCCCCACAGGCGCCCGTCCGCCTGCCCCCTGGTGGCCC
CCTGTTGCCCAGCGCCAGCCTGACTCGTGGGCCACAGCCGCCCCCGCTTGCAGTCACGGTACCGTCCTCT
CTCCCCCAGTCCCCCCCAGAGAACCCTGGCCAGCCATCGATGGGGATCGACATCGCCTCGGCGCCGGCTC
TGCAGCAGTACCGCACTAGCGCCGGCTCCCCGGCCAACCAGTCTCCCACCTCGCCAGTCTCCAATCAAGG
CTTCTCCCCAGGGAGCTCCCCGCAACACACTTCCACCCTGGGCAGCGTGTTTGGGGACGCGTACTATGAG
CAGCAGATGGCGGCCAGGCAGGCCAATGCTCTGTCCCACCAGCTGGAGCAGTTCAACATGATGGAGAACG
CCATCAGCTCCAGCAGCCTGTACAGCCCGGGCTCCACACTCAACTACTCGCAGGCGGCCATGATGGGCCT
CACGGGCAGCCACGGGAGCCTGCCGGACTCGCAGCAACTGGGATACGCCAGCCACAGTGGCATCCCCAAC
ATCATCCTCACAGTGACAGGAGAGTCCCCCCCCAGCCTCTCTAAAGAACTGACCAGCTCTCTGGCCGGGG
TCGGCGACGTCAGCTTCGACTCCGACAGCCAGTTTCCCCTGGACGAACTCAAGATCGACCCCCTGACCCT
CGACGGACTGCACATGCTCAACGACCCCGACATGGTTCTGGCCGACCCAGCCACCGAGGACACCTTCCGG
ATGGACCGCCTG

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC206974 representing NM_015321
Red=Cloning site Green=Tags(s)

MATSNNPRKFSEKIALHNQKQAEETAAFEEVMKDLSLTRAARLQLQKSQYLQLGPSRGQYYGGSLPNVNQ
IGSGTMDLPFQTPFQSSGLDTSRTTRHHGLVDRVYRERGRLGSPHRRPLSVDKHGRQADSCPYGTMYLSP
PADTSWRRTNSDSALHQSTMTPTQPESFSSGSQDVHQKRVLLLTVPGMEETTSEADKNLSKQAWDTKKTG
SRPKSCEVPGINIFPSADQENTTALIPATHNTGGSLPDLTNIHFPSPLPTPLDPEEPTFPALSSSSSTGN
LAANLTHLGIGGAGQGMSTPGSSPQHRPAGVSPLSLSTEARRQQASPALSPLSPITQAVAMDALSLEQQL
PYAFFTQAGSQQPPPQPQPPPPPPPASQQPPPPPPPQAPVRLPPGGPLLPSASLTRGPQPPPLAVTVPSS
LPQSPPENPGQPSMGIDIASAPALQQYRTSAGSPANQSPTSPVSNQGFSPGSSPQHTSTLGSVFGDAYYE
QQMAARQANALSHQLEQFNMMENAISSSSLYSPGSTLNYSQAAMMGLTGSHGSLPDSQQLGYASHSGIPN
IILTVTGESPPSLSKELTSSLAGVGDVSFDSDSQFPLDELKIDPLTLDGLHMLNDPDMVLADPATEDTFR
MDRL

SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_015321
ORF Size 1902 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015321.1, NP_056136.1
RefSeq Size 2505 bp
RefSeq ORF 1905 bp
Locus ID 23373
UniProt ID Q6UUV9
Cytogenetics 19p13.11
Protein Families Transcription Factors
MW 67.1 kDa
Summary Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells. In the hippocampus, involved in late-phase long-term potentiation (L-LTP) maintenance at the Schaffer collateral-CA1 synapses. May be required for dendritic growth of developing cortical neurons (By similarity). In concert with SIK1, regulates the light-induced entrainment of the circadian clock. In response to light stimulus, coactivates the CREB-mediated transcription of PER1 which plays an important role in the photic entrainment of the circadian clock.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_015321" in other vectors (6)
SKU Description Size Price
RC206974L1 Lenti ORF clone of Human CREB regulated transcription coactivator 1 (CRTC1), transcript variant 1, Myc-DDK-tagged 10 ug
$890.00
RC206974L2 Lenti ORF clone of Human CREB regulated transcription coactivator 1 (CRTC1), transcript variant 1, mGFP tagged 10 ug
$890.00
RC206974L3 Lenti ORF clone of Human CREB regulated transcription coactivator 1 (CRTC1), transcript variant 1, Myc-DDK-tagged 10 ug
$890.00
RC206974L4 Lenti ORF clone of Human CREB regulated transcription coactivator 1 (CRTC1), transcript variant 1, mGFP tagged 10 ug
$890.00
RG206974 CRTC1 (tGFP-tagged) - Human CREB regulated transcription coactivator 1 (CRTC1), transcript variant 1 10 ug
$790.00
SC322568 CRTC1 (untagged)-Human CREB regulated transcription coactivator 1 (CRTC1), transcript variant 1 10 ug
$591.00

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