ARMC3 (NM_173081) Human Tagged ORF Clone

CAT#: RC206951

ARMC3 (Myc-DDK-tagged)-Human armadillo repeat containing 3 (ARMC3)

Product Images

Circular map for RC206951

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  Western blot validation of overexpression lysate (Cat# LY406678) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206951 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ARMC3 protein (Cat# TP306951). The protein was produced from HEK293T cells transfected with ARMC3 cDNA clone (Cat# RC206951) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ARMC3
• Synonyms: CT81; KU-CT-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206951 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTAAAAAGATAAAGAAGGAAGTAGAGCCTCCTCCTAAGGATGTGTTTGACCCATTAATGATTGAAA
  GCAAAAAAGCAGCAACTGTGGTGTTAATGCTTAATTCTCCAGAAGAGGAAATTTTGGCTAAAGCATGTGA
  AGCCATTTATAAATTTGCTTTAAAAGGTGAGGAAAATAAAACAACCCTCCTTGAACTTGGAGCTGTGGAA
  CCTTTAACTAAGCTACTCACCCATGAAGACAAAATTGTAAGAAGAAATGCTACTATGATATTTGGAATCC
  TGGCTTCTAATAATGATGTTAAAAAATTGTTAAGGGAGTTAGATGTCATGAATTCTGTCATTGCCCAGCT
  CGCTCCAGAAGAAGAAGTAGTTATCCATGAGTTTGCTAGTCTTTGTCTAGCAAACATGTCTGCAGAGTAC
  ACCAGTAAAGTGCAAATATTTGAACATGGGGGATTAGAGCCACTCATCAGACTACTGAGTAGCCCTGACC
  CGGATGTAAAGAAGAACTCTATGGAATGCATTTACAACTTGGTGCAGGATTTTCAGTGTCGAGCTAAACT
  TCAAGAACTAAATGCAATACCTCCTATCTTAGATCTCTTGAAGTCAGAATATCCAGTGATTCAGTTGTTG
  GCTCTCAAAACCTTAGGTGTTATTGCAAATGATAAGGAGTCTCGAACAATGCTAAGAGACAATCAAGGAT
  TGGACCATCTTATTAAGATCCTAGAAACTAAGGAATTGAATGACCTTCATATAGAAGCACTTGCAGTGAT
  AGCCAATTGCCTTGAAGACATGGATACTATGGTGCAGATTCAGCAGACAGGGGGTCTTAAAAAGCTCCTG
  TCATTTGCAGAAAACTCTACAATTCCTGATATTCAGAAGAATGCAGCAAAAGCCATTACTAAAGCAGCTT
  ATGATCCTGAAAATAGAAAACTTTTTCATGAACAAGAGGTTGAAAAGTGCCTTGTAGCCCTTTTGGGTTC
  TGAAAATGATGGAACTAAAATTGCTGCTTCCCAAGCTATTTCAGCAATGTGTGAGAATTCAGGCAGCAAA
  GATTTTTTCAATAATCAGGGGATTCCACAGTTAATTCAGTTGCTAAAAAGTGACAATGAAGAGGTACGGG
  AAGCAGCAGCTCTAGCCCTGGCAAACCTAACCACTTGCAACCCTGCTAATGCAAACGCTGCTGCTGAAGC
  TGATGGTATTGATCCATTAATAAACCTCCTGTCTAGTAAACGAGATGGAGCCATTGCCAACGCTGCTACA
  GTATTAACAAACATGGCCATGCAGGAGCCCCTGCGCCTGAACATACAGAATCACGACATCATGCATGCCA
  TCATCAGCCCACTGCGTTCTGCAAACACAGTCGTGCAGAGCAAAGCTGCTCTCGCTGTCACCGCAACTGC
  GTGTGACGTTGAAGCCCGGACTGAGTTAAGAAATTCTGGTGGATTGGAGCCCCTGGTAGAGCTGCTACGC
  TCCAAGAATGATGAAGTGAGGAAGCACGCCAGTTGGGCAGTGATGGTCTGTGCTGGTGACGAGCTGACGG
  CCAATGAATTATGCAGGCTCGGGGCTTTAGATATCCTTGAAGAAGTTAACGTATCAGGAACTCGGAAAAA
  TAAATTCAGTGAGGCAGCTTATAATAAGTTGCTCAATAACAATCTTTCCCTGAAATACAGCCAGACTGGC
  TATTTGTCATCAAGTAACATAATTAACGATGGATTCTATGATTATGGTCGGATAAATCCCGGCACCAAAC
  TGTTGCCTTTGAAGGAGCTCTGCTTACAAGAACCAAGTGACCTACGGGCTGTACTCTTAATCAACAGTAA
  ATCTTACGTTTCTCCACCTTCATCTATGGAAGATAAATCAGATGTTGGTTATGGACAAAGTATTTCTTCT
  TCATCTTCCTTAAGAAGATCAAGTAAAGAAAAGAACAAAAAAAATAGTTATCATTTTAGTGCTGGATTTG
  GATCTCCCATAGAAGACAAATCAGAGCCAGCTTCTGGACGAAATACTGTTCTCAGCAAAAGCGCCACCAA
  AGAAAAAGGATGGAGGAAAAGCAAAGGAAAAAAAGAAGAGGAAAAAGTGAAAGAGGAGGAAGAGGTTATG
  GTGGTACCAAAATTTGTTGGTGAAGGAAGCTCTGACAAAGAATGGTGTCCTCCCTCTGACCCTGATTTCT
  CTATGTATGTGTATGAGGTGACCAAATCAATACTGCCAATAACCAATATTAAGGAACAGATTGAGGATCT
  GGCAAAGTATGTAGCAGAAAAAATGGGTGGTAAGATTCCAAAAGAGAAACTACCTGATTTCAGCTGGGAA
  CTTCACATAAGTGAACTGAAATTTCAACTTAAATCCAATGTTATACCGATTGGACATGTCAAAAAAGGAA
  TCTTCTACCATCGAGCTTTGCTTTTCAAGGCTCTGGCTGATAGAATTGGCATTGGTTGCTCCCTAGTTCG
  CGGAGAGTACGGTAGAGCGTGGAATGAAGTCATGCTGCAGAATGACTCTCGGAAGGGAGTGATTGGGGGC
  CTCCCCGCTCCTGAGATGTACGTGATTGACCTCATGTTCCATCCAGGTGGACTGATGAAGTTGAGAAGTC
  GAGAGGCTGATCTTTACAGATTCATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206951 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGKKIKKEVEPPPKDVFDPLMIESKKAATVVLMLNSPEEEILAKACEAIYKFALKGEENKTTLLELGAVE
  PLTKLLTHEDKIVRRNATMIFGILASNNDVKKLLRELDVMNSVIAQLAPEEEVVIHEFASLCLANMSAEY
  TSKVQIFEHGGLEPLIRLLSSPDPDVKKNSMECIYNLVQDFQCRAKLQELNAIPPILDLLKSEYPVIQLL
  ALKTLGVIANDKESRTMLRDNQGLDHLIKILETKELNDLHIEALAVIANCLEDMDTMVQIQQTGGLKKLL
  SFAENSTIPDIQKNAAKAITKAAYDPENRKLFHEQEVEKCLVALLGSENDGTKIAASQAISAMCENSGSK
  DFFNNQGIPQLIQLLKSDNEEVREAAALALANLTTCNPANANAAAEADGIDPLINLLSSKRDGAIANAAT
  VLTNMAMQEPLRLNIQNHDIMHAIISPLRSANTVVQSKAALAVTATACDVEARTELRNSGGLEPLVELLR
  SKNDEVRKHASWAVMVCAGDELTANELCRLGALDILEEVNVSGTRKNKFSEAAYNKLLNNNLSLKYSQTG
  YLSSSNIINDGFYDYGRINPGTKLLPLKELCLQEPSDLRAVLLINSKSYVSPPSSMEDKSDVGYGQSISS
  SSSLRRSSKEKNKKNSYHFSAGFGSPIEDKSEPASGRNTVLSKSATKEKGWRKSKGKKEEEKVKEEEEVM
  VVPKFVGEGSSDKEWCPPSDPDFSMYVYEVTKSILPITNIKEQIEDLAKYVAEKMGGKIPKEKLPDFSWE
  LHISELKFQLKSNVIPIGHVKKGIFYHRALLFKALADRIGIGCSLVRGEYGRAWNEVMLQNDSRKGVIGG
  LPAPEMYVIDLMFHPGGLMKLRSREADLYRFI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173081
• ORF Size: 2616 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173081.2
• RefSeq Size: 3765 bp
• RefSeq ORF: 2619 bp
• Locus ID: 219681
• UniProt ID: Q5W041
• Cytogenetics: 10p12.2
• MW: 96.4 kDa
• Gene Summary: Armadillo/beta-catenin (CTNNB1; MIM 116806)-like (ARM) domains are imperfect 45-amino acid repeats involved in protein-protein interactions. ARM domain-containing proteins, such as ARMC3, function in signal transduction, development, cell adhesion and mobility, and tumor initiation and metastasis (Li et al., 2006 [PubMed 16915934]).[supplied by OMIM, Mar 2008]