AGBL2 (NM_024783) Human Tagged ORF Clone

CAT#: RC206949

AGBL2 (Myc-DDK-tagged)-Human ATP/GTP binding protein-like 2 (AGBL2)

Product Images

Circular map for RC206949

• 

  Western blot validation of overexpression lysate (Cat# LY411047) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206949 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AGBL2
• Synonyms: CCP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206949 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCCAGCTTTGGAAACGCACCTAAAGCAGACTATTCCTGATCCTTATGAAGACTTTATGTACCGTC
  ACCTCCAATATTATGGCTACTTTAAAGCTCAGAGAGGCAGTTTACCAAACTCTGCTACGCATCAGCATGT
  TCGGAAGAATAACCCTCAATGCCTGTTGAATGGCTCTCTTGGGGAAAAAGATGATTTGATACCAGACACC
  CTGCAAAAGGAGAAGCTTCTATGGCCTATCAGTTTATCTTCAGCTGTGCACAGACAGATAGAAGCCATCA
  ACAGAGACTTTCACTCTTGCCTGGGCTGGATGCAGTGGCGCGGCCTCAGCTCACTGCAACCTCCACCTCC
  CAGATTCAAGGATTCTCCTGCCTCAGCTTTCCGAGTAGCTGGGATTACAGATTCACATATGCTGAGTTTA
  CCACATCTTAGGAGCAGACAGCTTCTTTATGATGAGTTGGATGAAGTAAACCCACGTCTTCGAGAACCCC
  AAGAGCTCTTTTCCATTTTGTCTACCAAGAGGCCACTGCAGGCTCCAAGATGGCCAATTGAATGTGAGGT
  CATCAAGGAAAACATCCATCATATTGAGTGGGCTCCACCTCAACCAGAATATTTCTATCAGCCTAAAGGA
  AATGAAAAGGTACCAGAGATTGTAGGAGAGAAAAAAGGAACAGTTGTCTATCAATTAGATTCAGTGCCTA
  TAGAAGGTTCCTATTTTACCAGTTCCAGAGTGGGAGGCAAACGAGGAATTGTCAAGGAACTTGCTGTCAC
  GTTGCAAGGACCAGAAGATAATACTCTACTGTTTGAATCAAGGTTTGAGAGTGGGAATCTGCAAAAAGCT
  GTCAGAGTAGACACCTATGAGTATGAACTCACCTTGCGAACTGACCTCTACACTAACAAACACACTCAGT
  GGTTTTATTTTCGTGTTCAGAACACCAGAAAAGATGCTACCTATCGCTTCACCATTGTCAACTTGCTAAA
  ACCCAAGAGTCTTTATACTGTAGGGATGAAGCCACTCTTGTACTCCCAATTGGATGCCAACACCCGCAAT
  ATTGGCTGGAGGAGAGAAGGAAATGAAATCAAGTACTACAAGAACAACACGGATGATGGGCAGCAGCCCT
  TCTACTGTCTCACGTGGACCATTCAGTTTCCATATGACCAGGACACTTGCTTCTTTGCACACTTCTACCC
  ATATACATACACTGATTTGCAATGCTACCTCCTGTCAGTGGCAAACAACCCTATCCAGTCTCAGTTCTGC
  AAGCTCCAAACTTTATGCAGGAGCCTAGCAGGAAATACCGTTTACTTGCTCACCATCACCAACCCATCCC
  AGACCCCTCAAGAGGCAGCTGCAAAGAAAGCTGTGGTCTTGAGTGCCAGAGTTCACCCTGGAGAAAGTAA
  TGGCTCCTGGGTTATGAAAGGCTTTTTGGACTTCATCCTTAGCAACTCCCCAGATGCCCAGCTCCTCAGA
  GATATTTTTGTCTTCAAGGTGCTTCCCATGTTAAATCCAGATGGTGTGATTGTGGGGAATTATCGGTGTT
  CCTTGGCCGGAAGGGATTTGAACAGGCATTATAAAACCATTCTGAAGGAGTCTTTCCCTTGTATTTGGTA
  CACCAGGAACATGATCAAAAGACTTCTTGAAGAAAGAGAGGTTCTGTTGTATTGTGATTTCCATGGCCAC
  AGTCGTAAGAATAATATCTTCCTGTATGGCTGTAATAACAACAATCGCAAATACTGGCTTCATGAACGAG
  TCTTTCCTTTAATGTTATGCAAAAATGCACCAGATAAGTTCTCTTTTCACAGTTGTAATTTTAAGGTCCA
  AAAATGCAAAGAAGGAACAGGACGAGTTGTTATGTGGCGGATGGGAATCCTAAACAGCTACACCATGGAG
  TCTACCTTTGGCGGGTCCACCCTGGGTAATAAAAGAGACACCCACTTTACCATCGAAGATCTGAAGTCCT
  TAGGTTATCATGTCTGTGACACCCTTCTGGACTTTTGTGATCCTGACCAAATTAAGTTCACTCAGTGTCT
  AGCAGAGCTTAAGGAGCTTTTACGACAGGAAATCCACAAGAAATTCCATGAACTTGGACAAGATGTAGAT
  TTAGAAGGAAGTTGGAGTGACATCTCTTTGTCTGACATTGAATCCAGCACCAGTGGCTCTGACAGTTCTC
  TCTCAGATGGTCTTCCTGTTCACCTAGCAAACATAGCAGATGAGCTGACTCAGAAAAAGAAGATGTTTAA
  GAAGAAAAAAAAGAAGTCACTTCAGACTAGGAAACAGCGAAATGAGCAGTATCAGAAAAAAAATTTGATG
  CAGAAGTTAAAGTTAACAGAAGATACCTCAGAAAAGGCAGGATTTGCTTCTACTCTGCAAAAGCAGCCAA
  CCTTTTTCAAAAACTCAGAGAATTCCAGTTTTTTACCAATGAAAAATGAAAACCCAAGGTTAAATGAGAC
  AAATTTAAATAGAAGAGACAAAGACACCCCCCTGGACCCATCAATGGCCACCCTGATTCTGCCTAAGAAT
  AAAGGGAGAATGCAGAATAAGAAGCCAGGCTTTACAGTATCATGCTCTCCAAAGAGAACCATAAACTCCA
  GCCAAGAGCCAGCTCCAGGTATGAAGCCAAACTGGCCTAGGAGCAGATATCCTGCCACAAAGAGAGGCTG
  TGCTGCCATGGCGGCATACCCATCCTTGCACATATACACATACCCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206949 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFPALETHLKQTIPDPYEDFMYRHLQYYGYFKAQRGSLPNSATHQHVRKNNPQCLLNGSLGEKDDLIPDT
  LQKEKLLWPISLSSAVHRQIEAINRDFHSCLGWMQWRGLSSLQPPPPRFKDSPASAFRVAGITDSHMLSL
  PHLRSRQLLYDELDEVNPRLREPQELFSILSTKRPLQAPRWPIECEVIKENIHHIEWAPPQPEYFYQPKG
  NEKVPEIVGEKKGTVVYQLDSVPIEGSYFTSSRVGGKRGIVKELAVTLQGPEDNTLLFESRFESGNLQKA
  VRVDTYEYELTLRTDLYTNKHTQWFYFRVQNTRKDATYRFTIVNLLKPKSLYTVGMKPLLYSQLDANTRN
  IGWRREGNEIKYYKNNTDDGQQPFYCLTWTIQFPYDQDTCFFAHFYPYTYTDLQCYLLSVANNPIQSQFC
  KLQTLCRSLAGNTVYLLTITNPSQTPQEAAAKKAVVLSARVHPGESNGSWVMKGFLDFILSNSPDAQLLR
  DIFVFKVLPMLNPDGVIVGNYRCSLAGRDLNRHYKTILKESFPCIWYTRNMIKRLLEEREVLLYCDFHGH
  SRKNNIFLYGCNNNNRKYWLHERVFPLMLCKNAPDKFSFHSCNFKVQKCKEGTGRVVMWRMGILNSYTME
  STFGGSTLGNKRDTHFTIEDLKSLGYHVCDTLLDFCDPDQIKFTQCLAELKELLRQEIHKKFHELGQDVD
  LEGSWSDISLSDIESSTSGSDSSLSDGLPVHLANIADELTQKKKMFKKKKKKSLQTRKQRNEQYQKKNLM
  QKLKLTEDTSEKAGFASTLQKQPTFFKNSENSSFLPMKNENPRLNETNLNRRDKDTPLDPSMATLILPKN
  KGRMQNKKPGFTVSCSPKRTINSSQEPAPGMKPNWPRSRYPATKRGCAAMAAYPSLHIYTYP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024783
• ORF Size: 2706 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024783.4
• RefSeq Size: 3613 bp
• RefSeq ORF: 2709 bp
• Locus ID: 79841
• UniProt ID: Q5U5Z8
• Cytogenetics: 11p11.2
• Protein Families: Druggable Genome
• MW: 104.2 kDa
• Gene Summary: Metallocarboxypeptidase that mediates deglutamylation of target proteins. Catalyzes the deglutamylation of polyglutamate side chains generated by post-translational polyglutamylation in proteins such as tubulins. Also removes gene-encoded polyglutamates from the carboxy-terminus of target proteins such as MYLK. Does not show detyrosinase or deglycylase activities from the carboxy-terminus of tubulin.[UniProtKB/Swiss-Prot Function]