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POLDIP1 (KCTD13) (NM_178863) Human Tagged ORF Clone

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SKU
RC206944
KCTD13 (Myc-DDK-tagged)-Human potassium channel tetramerisation domain containing 13 (KCTD13)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $450.00
In Stock*
Size
Bulk Requests & Clone Modifications

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Other products for "POLDIP1 or NM_178863"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol POLDIP1
Synonyms BACURD1; FKSG86; hBACURD1; PDIP1; POLDIP1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206944 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGGCGGAGGCCTCGGGCCCGGCTGCCGCCGCGGCCCCGTCCCTGGAAGCCCCCAAGCCCTCGGGTC
TCGAGCCTGGCCCCGCCGCCTACGGTCTCAAGCCGCTGACCCCGAACAGCAAATACGTGAAGCTGAACGT
GGGCGGCTCGTTGCACTACACCACGCTGCGCACCCTCACGGGACAGGACACCATGCTCAAAGCCATGTTC
AGCGGCCGCGTGGAGGTGCTGACCGATGCCGGAGGTTGGGTGCTGATTGACCGGAGCGGCCGTCACTTTG
GTACAATCCTCAATTACCTGCGGGATGGGTCTGTGCCACTGCCGGAGAGTACGAGAGAACTGGGGGAGCT
GCTGGGCGAAGCACGCTACTACCTGGTGCAGGGCCTGATTGAGGACTGCCAGCTGGCGCTGCAGCAAAAA
AGGGAGACGCTGTCCCCGCTGTGCCTCATCCCCATGGTGACATCTCCCCGGGAGGAGCAGCAGCTCCTGG
CCAGCACCTCCAAGCCCGTGGTGAAGCTCCTGCACAACCGCAGTAACAACAAGTACTCCTACACCAGCAC
TTCAGATGACAACCTACTTAAGAACATCGAGCTGTTCGACAAGCTGGCCCTGCGCTTCCACGGGCGGCTA
CTCTTCCTCAAGGATGTCCTGGGGGACGAGATCTGTTGCTGGTCTTTCTACGGGCAGGGCCGCAAAATCG
CCGAGGTGTGCTGCACCTCCATTGTCTATGCTACGGAGAAGAAGCAGACCAAGGTGGAATTTCCAGAGGC
CCGGATCTTCGAGGAGACCCTGAACATCCTCATCTACGAGACTCCCCGGGGCCCAGACCCAGCCCTCCTG
GAGGCCACAGGGGGAGCAGCTGGAGCTGGTGGGGCTGGCCGCGGGGAGGATGAAGAGAACCGAGAGCACC
GTGTCCGCAGGATCCATGTCCGGCGCCATATCACCCACGACGAGCGTCCTCATGGCCAACAAATTGTCTT
CAAGGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC206944 protein sequence
Red=Cloning site Green=Tags(s)

MSAEASGPAAAAAPSLEAPKPSGLEPGPAAYGLKPLTPNSKYVKLNVGGSLHYTTLRTLTGQDTMLKAMF
SGRVEVLTDAGGWVLIDRSGRHFGTILNYLRDGSVPLPESTRELGELLGEARYYLVQGLIEDCQLALQQK
RETLSPLCLIPMVTSPREEQQLLASTSKPVVKLLHNRSNNKYSYTSTSDDNLLKNIELFDKLALRFHGRL
LFLKDVLGDEICCWSFYGQGRKIAEVCCTSIVYATEKKQTKVEFPEARIFEETLNILIYETPRGPDPALL
EATGGAAGAGGAGRGEDEENREHRVRRIHVRRHITHDERPHGQQIVFKD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178863
ORF Size 987 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178863.2, NP_849194.1
RefSeq Size 1745 bp
RefSeq ORF 990 bp
Locus ID 253980
UniProt ID Q8WZ19
Cytogenetics 16p11.2
Protein Families Ion Channels: Other, Transcription Factors
MW 36.4 kDa
Summary Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for synaptic transmission (PubMed:19782033). The BCR(KCTD13) E3 ubiquitin ligase complex mediates the ubiquitination of RHOA, leading to its degradation by the proteasome (PubMed:19782033) Degradation of RHOA regulates the actin cytoskeleton and promotes synaptic transmission (By similarity).[UniProtKB/Swiss-Prot Function]
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Documents
Resources
"NM_178863" in other vectors (6)
SKU Description Size Price
RC206944L1 Lenti ORF clone of Human potassium channel tetramerisation domain containing 13 (KCTD13), Myc-DDK-tagged 10 ug
$750.00
RC206944L2 Lenti ORF clone of Human potassium channel tetramerisation domain containing 13 (KCTD13), mGFP tagged 10 ug
$750.00
RC206944L3 Lenti ORF clone of Human potassium channel tetramerisation domain containing 13 (KCTD13), Myc-DDK-tagged 10 ug
$750.00
RC206944L4 Lenti ORF clone of Human potassium channel tetramerisation domain containing 13 (KCTD13), mGFP tagged 10 ug
$750.00
RG206944 KCTD13 (tGFP-tagged) - Human potassium channel tetramerisation domain containing 13 (KCTD13) 10 ug
$650.00
SC107237 KCTD13 (untagged)-Human potassium channel tetramerisation domain containing 13 (KCTD13) 10 ug
$450.00

file-alt Citations

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