Fbx15 (FBXO15) (NM_152676) Human Tagged ORF Clone

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SKU
RC206934
FBXO15 (Myc-DDK-tagged)-Human F-box protein 15 (FBXO15), transcript variant 1
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Fbx15
Synonyms FBX15
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206934 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTTCAGAAATCTTGCTGAAGATATTTTCCTACTTGGATGCTGTGAGCCTTCTGTGTACTGGATGTG
TGAGCAGGCGCTTTTATCATCTAGCCAATGACAATTTTATTTGGATCGGAATCTACTCAACTGCTTTTTC
ACCTGCAAGATCAAATTGGAAATTTAATTCAGTAGAGAAGATAGCTATGTCTATGAGCTTTCTGTCAGTT
CAGGATAAAGAAGCTGGTTATTGGAAGAAAGAATATATCACAAAACAAATAGCATCTGTAAAAGCCGCAC
TAGCTGACATTCTCAAACCTGTCAACCCTTACACAGGCCTTCCAGTTAAGACCAAAGAGGCCCTCAGAAT
ATTTGGTTTAGGTTGGGCAATTATACTGAAAGAAAAAGGTGGAAAAGAATATATCATGGAGCATGTTGAT
CTTTCCATAAATGACACATCAGTTACTGTTATATGGTATGGCAAAAAATGGCCATGCCTAGCATCATTGT
CAACCTTAGATTTATGTGGCATGACACCAGTTTTTACCGACTGGTATAAAACTCCCACCAAACATAGACT
CCGATGGCATTCTTTAATTGCAAAGTACAATCTGAGTCATTTGACCATATCTACCATGATTGGCTGTGAC
AGACTCATTCGGATCTTCTGCCTGCACCCTGGCCTCCTGGTGGGAGTGTGGAAGAAGGAGGAAGAACTGG
CTTTTGTTATGGCAAATCTTCATTTTCATCACCTTGTGGAGAGGAGCACATTAGGCTCGGCTACTATCCC
CTATGAACTGCCTCCACATAGCCCCTTTTTGGATGATAGCCCCGAGTATGGACTGCACGGCTACCAACTC
CATGTTGATCTGCACAGCGGTGGGGTTTTCTACCTATGTGGTACATTTCGCAATCTCTTCACCAAGAGAG
GAAATATTGAAAATGGACATGTGAAGCTCATTGTTATACATTTAAAAAATAACAGAGAACACCTACCTCT
TATTGGAAAAGTTGGCCTCTCGTGGAAAACTGATATTTTTGATGGCTGTATAAAGAGTTGTTCCATGATG
GACGTAACTCTTTTGGATGAACATGGGAAACCCTTTTGGTGTTTCAGTTCCCCGGTGTGCCTGAGATCGC
CTGCCACACCCTCTGACAGCTCTAGCTTCTTGGGACAGACATACAACGTGGACTACGTTGATGCGGAAGG
AAGAGTGCACGTGGAGCTGGTGTGGATCAGAGAGACCGAAGAATACCTTATTGTCAACCTGGTCCTTTAT
CTTAGTATCGCAAAAATCAACCATTGGTTTGGGACTGAATAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC206934 protein sequence
Red=Cloning site Green=Tags(s)

MPSEILLKIFSYLDAVSLLCTGCVSRRFYHLANDNFIWIGIYSTAFSPARSNWKFNSVEKIAMSMSFLSV
QDKEAGYWKKEYITKQIASVKAALADILKPVNPYTGLPVKTKEALRIFGLGWAIILKEKGGKEYIMEHVD
LSINDTSVTVIWYGKKWPCLASLSTLDLCGMTPVFTDWYKTPTKHRLRWHSLIAKYNLSHLTISTMIGCD
RLIRIFCLHPGLLVGVWKKEEELAFVMANLHFHHLVERSTLGSATIPYELPPHSPFLDDSPEYGLHGYQL
HVDLHSGGVFYLCGTFRNLFTKRGNIENGHVKLIVIHLKNNREHLPLIGKVGLSWKTDIFDGCIKSCSMM
DVTLLDEHGKPFWCFSSPVCLRSPATPSDSSSFLGQTYNVDYVDAEGRVHVELVWIRETEEYLIVNLVLY
LSIAKINHWFGTEY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_152676
ORF Size 1302 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_152676.3
RefSeq Size 1767 bp
RefSeq ORF 1305 bp
Locus ID 201456
UniProt ID Q8NCQ5
Cytogenetics 18q22.3
Domains F-box
Protein Families Druggable Genome
MW 49.5 kDa
Summary Members of the F-box protein family, such as FBXO15, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 PubMed 15520277).supplied by OMIM, Mar 2008
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Documents
Resources
"NM_152676" in other vectors (6)
SKU Description Size Price
RC206934L1 Lenti ORF clone of Human F-box protein 15 (FBXO15), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC206934L2 Lenti ORF clone of Human F-box protein 15 (FBXO15), transcript variant 1, mGFP tagged 10 ug
$757.00
RC206934L3 Lenti ORF clone of Human F-box protein 15 (FBXO15), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC206934L4 Lenti ORF clone of Human F-box protein 15 (FBXO15), transcript variant 1, mGFP tagged 10 ug
$757.00
RG206934 FBXO15 (tGFP-tagged) - Human F-box protein 15 (FBXO15), transcript variant 1 10 ug
$657.00
SC100626 FBXO15 (untagged)-Human F-box protein 15 (FBXO15), transcript variant 1 10 ug
$457.00

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