OriGene Logo

DYRK4 (NM_003845) Human Tagged ORF Clone

  • Product Brand Image
Be the first to review this product
SKU
RC206929
DYRK4 (Myc-DDK-tagged)-Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4 (DYRK4)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $484.00
In Stock*
Size
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

TA375678 DYRK4 Rabbit polyclonal Antibody 100 ul
$410.00
TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags 30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA) 5 x 200 ul
$160.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each 100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug 10 ug
$650.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL 5 ml
$720.00

Other products for "DYRK4 or NM_003845"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DYRK4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206929 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGGCCTCAGAGCTCAAGGCTTCAGAAATACCTTTCCACCCTAGCATTAAAACCCAGGATCCCAAGG
CAGAGGAGAAGTCACCAAAGAAGCAAAAGGTGACTCTGACAGCGGCAGAGGCCCTAAAGCTTTTTAAGAA
CCAGCTGTCTCCATATGAACAAAGTGAAATCCTGGGCTACGCGGAGCTGTGGTTCCTGGGTCTTGAAGCC
AAGAAGCTCGACACGGCTCCTGAGAAATTTAGCAAGACGAGTTTTGATGATGAGCATGGCTTCTATCTGA
AGGTTCTGCATGATCACATTGCCTACCGCTATGAAGTTCTGGAGACAATCGGGAAGGGGTCCTTTGGACA
GGTGGCCAAGTGCTTGGATCACAAAAACAATGAGCTGGTGGCCCTGAAAATCATCAGGAACAAGAAGAGG
TTTCACCAGCAGGCCCTGATGGAGCTGAAGATCCTGGAAGCTCTCAGAAAGAAGGACAAAGACAACACCT
ACAATGTGGTGCATATGAAGGACTTTTTCTACTTTCGCAATCACTTCTGCATCACCTTTGAGCTCCTGGG
AATCAACTTGTATGAGTTGATGAAGAATAACAACTTTCAAGGCTTCAGTCTGTCCATAGTTCGGCGCTTC
ACTCTCTCTGTTTTGAAGTGCTTGCAGATGCTTTCGGTAGAGAAAATCATTCACTGTGATCTCAAGCCCG
AAAATATAGTGCTATACCAAAAGGGCCAAGCCTCTGTTAAAGTCATTGACTTTGGATCAAGCTGTTATGA
ACACCAGAAAGTATACACGTACATCCAAAGCCGGTTCTACCGATCCCCAGAAGTGATCCTGGGCCACCCC
TACGACGTGGCCATTGACATGTGGAGCCTGGGCTGCATCACGGCGGAGTTGTACACGGGCTACCCCCTGT
TCCCCGGGGAGAATGAGGTGGAGCAGCTGGCCTGCATCATGGAGGTGCTGGGTCTGCCGCCAGCCGGCTT
CATTCAGACAGCCTCCAGGAGACAGACATTCTTTGATTCCAAAGGTTTTCCTAAAAATATAACCAACAAC
AGGGGGAAAAAAAGATACCCAGATTCCAAGGACCTCACGATGGTGCTGAAAACCTATGACACCAGCTTCC
TGGACTTTCTCAGAAGGTGTTTGGTATGGGAACCTTCTCTTCGCATGACCCCGGACCAGGCCCTCAAGCA
TGCTTGGATTCATCAGTCTCGGAACCTCAAGCCACAGCCCAGGCCCCAGACCCTGAGGAAATCCAATTCC
TTTTTCCCCTCTGAGACAAGGAAGGACAAGGTTCAAGGCTGTCATCACTCGAGCAGAAAAGCAGATGAGA
TCACCAAAGAGACTACAGAGAAAACAAAAGATAGCCCCACGAAGCATGTTCAGCATTCAGGTGATCAGCA
GGACTGTCTCCAGCACGGAGCTGACACTGTTCAGCTGCCTCAACTGGTAGACGCTCCCAAGAAGTCAGAG
GCAGCTGTCGGGGCGGAGGTGTCCATGACCTCCCCAGGACAGAGCAAAAACTTCTCCCTCAAGAACACAA
ACGTTTTACCCCCTATTGTA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC206929 protein sequence
Red=Cloning site Green=Tags(s)

MPASELKASEIPFHPSIKTQDPKAEEKSPKKQKVTLTAAEALKLFKNQLSPYEQSEILGYAELWFLGLEA
KKLDTAPEKFSKTSFDDEHGFYLKVLHDHIAYRYEVLETIGKGSFGQVAKCLDHKNNELVALKIIRNKKR
FHQQALMELKILEALRKKDKDNTYNVVHMKDFFYFRNHFCITFELLGINLYELMKNNNFQGFSLSIVRRF
TLSVLKCLQMLSVEKIIHCDLKPENIVLYQKGQASVKVIDFGSSCYEHQKVYTYIQSRFYRSPEVILGHP
YDVAIDMWSLGCITAELYTGYPLFPGENEVEQLACIMEVLGLPPAGFIQTASRRQTFFDSKGFPKNITNN
RGKKRYPDSKDLTMVLKTYDTSFLDFLRRCLVWEPSLRMTPDQALKHAWIHQSRNLKPQPRPQTLRKSNS
FFPSETRKDKVQGCHHSSRKADEITKETTEKTKDSPTKHVQHSGDQQDCLQHGADTVQLPQLVDAPKKSE
AAVGAEVSMTSPGQSKNFSLKNTNVLPPIV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_003845
ORF Size 1560 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003845.1, NP_003836.1
RefSeq Size 1864 bp
RefSeq ORF 1563 bp
Locus ID 8798
UniProt ID Q9NR20
Cytogenetics 12p13.32
Protein Families Druggable Genome, Protein Kinase
MW 59.6 kDa
Summary This gene encodes an enzyme that belongs to a conserved family of serine/threonine protein kinases. Members of this dual specificity kinase family are thought to function in the regulation of cell differentiation and proliferation, survival, and in development. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Aug 2013]
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Documents
Resources
"NM_003845" in other vectors (7)
SKU Description Size Price
RC206929L1 Lenti ORF clone of Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4 (DYRK4), Myc-DDK-tagged 10 ug
$784.00
RC206929L2 Lenti ORF clone of Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4 (DYRK4), mGFP tagged 10 ug
$784.00
RC206929L3 Lenti ORF clone of Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4 (DYRK4), Myc-DDK-tagged 10 ug
$784.00
RC206929L4 Lenti ORF clone of Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4 (DYRK4), mGFP tagged 10 ug
$784.00
RG206929 DYRK4 (tGFP-tagged) - Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4 (DYRK4) 10 ug
$489.00 $684.00
SC106952 DYRK4 (untagged)-Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4 (DYRK4) 10 ug
$485.00
SC323557 DYRK4 (untagged)-Kinase deficient mutant (K133M) of Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4 (DYRK4) 10 ug
$485.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.