DLX1 (NM_178120) Human Tagged ORF Clone

CAT#: RC206895

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DLX1 (Myc-DDK-tagged)-Human distal-less homeobox 1 (DLX1), transcript variant 1



  "NM_178120" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 300.00

USD 450.00


Availability*
In Stock

Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol DLX1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC206895 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCATGACCACCATGCCAGAAAGTCTCAACAGCCCCGTGTCGGGCAAGGCGGTGTTTATGGAGTTTG
GGCCGCCCAACCAGCAAATGTCTCCTTCTCCCATGTCCCACGGGCACTACTCCATGCACTGTTTACACTC
GGCGGGCCATTCGCAGCCCGACGGCGCCTACAGCTCAGCCTCGTCCTTCTCCCGACCGCTGGGCTACCCC
TACGTCAACTCGGTCAGCAGCCACGCATCCAGCCCCTACATCAGTTCGGTGCAGTCCTACCCGGGCAGCG
CCAGCCTCGCCCAGAGCCGCCTGGAGGACCCAGGGGCGGACTCGGAGAAGAGCACGGTGGTGGAAGGCGG
TGAAGTGCGCTTCAATGGCAAGGGAAAAAAGATCCGTAAACCCAGGACGATTTATTGCAGTTTGCAGTTG
CAGGCTTTGAACCGGAGGTTCCAGCAAACTCAGTACCTAGCTCTGCCGGAGAGGGCGGAGCTCGCGGCCT
CTTTGGGACTCACACAGACTCAGGTCAAGATCTGGTTCCAAAACAAGCGATCCAAGTTCAAGAAGCTGAT
GAAGCAGGGTGGGGCGGCTCTGGAGGGTAGTGCGTTGGCCAACGGTCGGGCCCTGTCTGCTGGCTCCCCA
CCCGTGCCGCCCGGCTGGAACCCTAACTCTTCATCCGGGAAGGGCTCAGGAGGAAACGCGGGCTCCTATA
TCCCCAGCTACACATCGTGGTACCCTTCAGCGCACCAAGAAGCTATGCAGCAACCCCAACTTATG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC206895 protein sequence
Red=Cloning site Green=Tags(s)

MTMTTMPESLNSPVSGKAVFMEFGPPNQQMSPSPMSHGHYSMHCLHSAGHSQPDGAYSSASSFSRPLGYP
YVNSVSSHASSPYISSVQSYPGSASLAQSRLEDPGADSEKSTVVEGGEVRFNGKGKKIRKPRTIYCSLQL
QALNRRFQQTQYLALPERAELAASLGLTQTQVKIWFQNKRSKFKKLMKQGGAALEGSALANGRALSAGSP
PVPPGWNPNSSSGKGSGGNAGSYIPSYTSWYPSAHQEAMQQPQLM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_178120
ORF Size 765 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_178120.5
RefSeq Size 2403 bp
RefSeq ORF 768 bp
Locus ID 1745
UniProt ID P56177
Cytogenetics 2q31.1
Protein Families ES Cell Differentiation/IPS, Transcription Factors
MW 27.3 kDa
Gene Summary This gene encodes a member of a homeobox transcription factor gene family similiar to the Drosophila distal-less gene. The encoded protein is localized to the nucleus where it may function as a transcriptional regulator of signals from multiple TGF-{beta} superfamily members. The encoded protein may play a role in the control of craniofacial patterning and the differentiation and survival of inhibitory neurons in the forebrain. This gene is located in a tail-to-tail configuration with another member of the family on the long arm of chromosome 2. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

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