Islet 1 (ISL1) (NM_002202) Human Tagged ORF Clone

CAT#: RC206893

ISL1 (Myc-DDK-tagged)-Human ISL LIM homeobox 1 (ISL1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ISL1 (Cat# RC206893, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ISL1 (1:500) (Cat# TA807585). Positive lysates LY400801 (100ug) and LC400801 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY400801) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206893 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Islet 1
• Synonyms: Isl-1; ISLET1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206893 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGACATGGGAGATCCACCAAAAAAAAAACGTCTGATTTCCCTATGTGTTGGTTGCGGCAATCAGA
  TTCACGATCAGTATATTCTGAGGGTTTCTCCGGATTTGGAATGGCATGCGGCATGTTTGAAATGTGCGGA
  GTGTAATCAGTATTTGGACGAGAGCTGTACATGCTTTGTTAGGGATGGGAAAACCTACTGTAAAAGAGAT
  TATATCAGGTTGTACGGGATCAAATGCGCCAAGTGCAGCATCGGCTTCAGCAAGAACGACTTCGTGATGC
  GTGCCCGCTCCAAGGTGTATCACATCGAGTGTTTCCGCTGTGTGGCCTGCAGCCGCCAGCTCATCCCTGG
  GGACGAATTTGCGCTTCGGGAGGACGGTCTCTTCTGCCGAGCAGACCACGATGTGGTGGAGAGGGCCAGT
  CTAGGCGCTGGCGACCCGCTCAGTCCCCTGCATCCAGCGCGGCCACTGCAAATGGCAGCGGAGCCCATCT
  CCGCCAGGCAGCCGGCCCTGCGGCCCCACGTCCACAAGCAGCCGGAGAAGACCACCCGCGTGCGGACTGT
  GCTGAACGAGAAGCAGCTGCACACCTTGCGGACCTGCTACGCCGCAAACCCGCGGCCAGATGCGCTCATG
  AAGGAGCAACTGGTAGAGATGACGGGCCTCAGTCCCCGTGTGATCCGGGTCTGGTTTCAAAACAAGCGGT
  GCAAGGACAAGAAGCGAAGCATCATGATGAAGCAACTCCAGCAGCAGCAGCCCAATGACAAAACTAATAT
  CCAGGGGATGACAGGAACTCCCATGGTGGCTGCCAGTCCAGAGAGACACGACGGTGGCTTACAGGCTAAC
  CCAGTGGAAGTACAAAGTTACCAGCCACCTTGGAAAGTACTGAGCGACTTCGCCTTGCAGAGTGACATAG
  ATCAGCCTGCTTTTCAGCAACTGGTCAATTTTTCAGAAGGAGGACCGGGCTCTAATTCCACTGGCAGTGA
  AGTAGCATCAATGTCCTCTCAACTTCCAGATACACCTAACAGCATGGTAGCCAGTCCTATTGAGGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206893 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGDMGDPPKKKRLISLCVGCGNQIHDQYILRVSPDLEWHAACLKCAECNQYLDESCTCFVRDGKTYCKRD
  YIRLYGIKCAKCSIGFSKNDFVMRARSKVYHIECFRCVACSRQLIPGDEFALREDGLFCRADHDVVERAS
  LGAGDPLSPLHPARPLQMAAEPISARQPALRPHVHKQPEKTTRVRTVLNEKQLHTLRTCYAANPRPDALM
  KEQLVEMTGLSPRVIRVWFQNKRCKDKKRSIMMKQLQQQQPNDKTNIQGMTGTPMVAASPERHDGGLQAN
  PVEVQSYQPPWKVLSDFALQSDIDQPAFQQLVNFSEGGPGSNSTGSEVASMSSQLPDTPNSMVASPIEA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002202
• ORF Size: 1047 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002202.2, NP_002193.2
• RefSeq Size: 2729 bp
• RefSeq ORF: 1050 bp
• Locus ID: 3670
• UniProt ID: P61371
• Cytogenetics: 5q11.1
• Protein Families: ES Cell Differentiation/IPS, Transcription Factors
• MW: 39 kDa
• Gene Summary: This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young. [provided by RefSeq, Jul 2008]