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PLA2G3 (NM_015715) Human Tagged Lenti ORF Clone

  • LentiORF®
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SKU
RC206872L2
Lenti ORF clone of Human phospholipase A2, group III (PLA2G3), mGFP tagged
ORF Plasmid:
mGFP DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $774.00
3 Weeks*
Size
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PLA2G3
Synonyms GIII-SPLA2; sPLA2-III; SPLA2III
Vector pLenti-C-mGFP
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection None
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC206872).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_015715
ORF Size 1527 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015715.2
RefSeq Size 2717 bp
RefSeq ORF 1530 bp
Locus ID 50487
UniProt ID Q9NZ20
Cytogenetics 22q12.2
Protein Families Druggable Genome, Secreted Protein
Protein Pathways alpha-Linolenic acid metabolism, Arachidonic acid metabolism, Ether lipid metabolism, Fc epsilon RI signaling pathway, Glycerophospholipid metabolism, GnRH signaling pathway, Linoleic acid metabolism, Long-term depression, MAPK signaling pathway, Metabolic pathways, Vascular smooth muscle contraction, VEGF signaling pathway
MW 57.2 kDa
Summary This gene encodes a protein that belongs to the secreted phospholipase A2 family, whose members include the bee venom enzyme. The encoded enzyme functions in lipid metabolism and catalyzes the calcium-dependent hydrolysis of the sn-2 acyl bond of phospholipids to release arachidonic acid and lysophospholipids. This enzyme acts as a negative regulator of ciliogenesis, and may play a role in cancer development by stimulating tumor cell growth and angiogenesis. This gene is associated with oxidative stress, and polymorphisms in this gene are linked to risk for Alzheimer's disease. provided by RefSeq, Apr 2014
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Documents
Resources
"NM_015715" in other vectors (6)
SKU Description Size Price
RC206872 PLA2G3 (Myc-DDK-tagged)-Human phospholipase A2, group III (PLA2G3) 10 ug
$289.00 $474.00
RC206872L1 Lenti ORF clone of Human phospholipase A2, group III (PLA2G3), Myc-DDK-tagged 10 ug
$774.00
RC206872L3 Lenti ORF clone of Human phospholipase A2, group III (PLA2G3), Myc-DDK-tagged 10 ug
$774.00
RC206872L4 Lenti ORF clone of Human phospholipase A2, group III (PLA2G3), mGFP tagged 10 ug
$774.00
RG206872 PLA2G3 (tGFP-tagged) - Human phospholipase A2, group III (PLA2G3) 10 ug
$489.00 $674.00
SC122829 PLA2G3 (untagged)-Human phospholipase A2, group III (PLA2G3) 10 ug
$475.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.