SNRPA1 (NM_003090) Human Tagged ORF Clone

CAT#: RC206856

SNRPA1 (Myc-DDK-tagged)-Human small nuclear ribonucleoprotein polypeptide A' (SNRPA1)

Product Images

Circular map for RC206856

• 

  Western blot validation of overexpression lysate (Cat# LY418901) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206856 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SNRPA1
• Synonyms: Lea1; U2A'
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206856 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCAAGCTGACGGCGGAGCTGATCGAGCAGGCGGCGCAGTACACCAACGCGGTGCGCGACCGGGAGC
  TGGACCTCCGGGGGTATAAAATTCCCGTCATTGAAAATCTAGGTGCTACGTTAGACCAGTTTGATGCTAT
  TGATTTTTCTGACAATGAGATCAGGAAACTGGATGGTTTTCCTTTGTTGAGAAGACTGAAAACATTGTTA
  GTGAACAACAACAGAATATGCCGTATAGGTGAGGGACTTGATCAGGCTCTGCCCTGTCTGACAGAACTCA
  TTCTCACCAATAATAGTCTCGTGGAACTGGGTGATCTGGACCCTCTGGCATCTCTCAAATCGCTGACTTA
  CCTAAGTATCCTAAGAAATCCGGTAACCAATAAGAAGCATTACAGATTGTATGTGATTTATAAAGTTCCG
  CAAGTCAGAGTACTGGATTTCCAGAAAGTGAAACTAAAAGAGCGTCAGGAAGCAGAGAAAATGTTCAAGG
  GCAAACGGGGTGCACAGCTTGCAAAGGATATTGCCAGGAGAAGCAAAACTTTTAATCCAGGTGCTGGTTT
  GCCAACTGACAAAAAGAAAGGTGGGCCATCTCCAGGGGATGTAGAAGCAATCAAGAATGCCATAGCAAAT
  GCTTCAACTCTGGCTGAAGTGGAGAGGCTGAAGGGGTTGCTGCAGTCTGGTCAGATCCCTGGCAGAGAAC
  GCAGATCAGGGCCCACTGATGATGGTGAAGAAGAGATGGAAGAAGACACAGTCACAAACGGGTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206856 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVKLTAELIEQAAQYTNAVRDRELDLRGYKIPVIENLGATLDQFDAIDFSDNEIRKLDGFPLLRRLKTLL
  VNNNRICRIGEGLDQALPCLTELILTNNSLVELGDLDPLASLKSLTYLSILRNPVTNKKHYRLYVIYKVP
  QVRVLDFQKVKLKERQEAEKMFKGKRGAQLAKDIARRSKTFNPGAGLPTDKKKGGPSPGDVEAIKNAIAN
  ASTLAEVERLKGLLQSGQIPGRERRSGPTDDGEEEMEEDTVTNGS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003090
• ORF Size: 765 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003090.4
• RefSeq Size: 1068 bp
• RefSeq ORF: 768 bp
• Locus ID: 6627
• UniProt ID: P09661
• Cytogenetics: 15q26.3
• Domains: LRR, LRRcap
• Protein Families: Druggable Genome
• Protein Pathways: Spliceosome
• MW: 28.4 kDa
• Gene Summary: Involved in pre-mRNA splicing as component of the spliceosome (PubMed:11991638, PubMed:28502770, PubMed:28781166, PubMed:28076346, PubMed:27035939). Associated with sn-RNP U2, where it contributes to the binding of stem loop IV of U2 snRNA (PubMed:9716128, PubMed:27035939).[UniProtKB/Swiss-Prot Function]