OGFOD1 (NM_018233) Human Tagged ORF Clone

CAT#: RC206839

OGFOD1 (Myc-DDK-tagged)-Human 2-oxoglutarate and iron-dependent oxygenase domain containing 1 (OGFOD1)

Product Images

Circular map for RC206839

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY OGFOD1 (Cat# RC206839, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OGFOD1(Cat# TA502364). Positive lysates LY413213 (100ug) and LC413213 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY413213) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206839 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified OGFOD1 protein (Cat# TP306839). The protein was produced from HEK293T cells transfected with OGFOD1 cDNA clone (Cat# RC206839) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: OGFOD1
• Synonyms: TPA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206839 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGGGAAGCGGCCAGCGGAGCCCGGCCCAGCCCGGGTGGGAAAAAAGGGAAAGAAGGAGGTGATGG
  CGGAGTTTTCGGACGCTGTTACGGAAGAAACCTTGAAAAAGCAGGTGGCTGAGGCCTGGAGCCGCAGGAC
  GCCGTTCAGTCACGAAGTCATTGTCATGGACATGGACCCTTTTCTTCACTGTGTGATCCCAAACTTCATC
  CAAAGCCAAGACTTCTTAGAAGGGCTTCAGAAGGAACTGATGAACTTGGACTTCCATGAGAAGTATAATG
  ATTTATATAAGTTCCAGCAGTCTGATGATTTGAAGAAGAGAAGAGAGCCTCACATCTCCACTTTAAGGAA
  AATTCTGTTTGAAGATTTCCGGTCCTGGCTTTCTGATATTTCTAAAATTGACCTGGAATCAACCATTGAC
  ATGTCCTGTGCTAAATATGAATTCACTGATGCCCTGCTGTGCCATGATGATGAGCTGGAAGGGCGCCGGA
  TTGCCTTCATCCTGTACCTGGTTCCTCCCTGGGACAGGAGCATGGGTGGTACCCTGGACCTGTACAGCAT
  TGATGAACACTTTCAGCCGAAGCAGATTGTCAAGTCTCTTATCCCTTCGTGGAACAAACTGGTTTTCTTT
  GAAGTATCTCCTGTGTCCTTTCACCAGGTGTCTGAAGTGCTGTCTGAAGAAAAGTCACGTTTGTCTATAA
  GTGGCTGGTTTCATGGTCCATCATTGACTCGGCCTCCCAACTACTTTGAACCCCCCATACCTCGGAGCCC
  TCACATCCCACAAGATCATGAGATTTTGTATGATTGGATCAACCCTACTTATCTGGACATGGATTACCAA
  GTTCAAATTCAAGAAGAGTTTGAAGAAAGTTCTGAAATTCTCCTGAAGGAGTTTCTTAAGCCTGAGAAAT
  TCACGAAAGTCTGTGAGGCCTTGGAGCATGGACATGTGGAATGGAGCAGCCGAGGTCCCCCTAACAAAAG
  GTTTTATGAGAAAGCTGAGGAGAGTAAGCTTCCTGAGATATTGAAGGAGTGCATGAAGTTATTTCGCTCT
  GAGGCACTATTCTTGCTGCTCTCCAACTTCACAGGCCTGAAGCTTCATTTCTTGGCCCCTTCGGAAGAAG
  ATGAGATGAATGATAAAAAAGAGGCAGAAACCACTGATATCACTGAAGAAGGGACTAGCCATAGTCCTCC
  TGAGCCAGAGAATAATCAGATGGCCATCAGCAACAACAGCCAACAGAGCAATGAGCAGACAGACCCAGAG
  CCAGAGGAAAATGAAACAAAGAAAGAATCAAGTGTTCCCATGTGCCAAGGGGAACTGAGGCATTGGAAGA
  CCGGTCACTACACTTTAATTCATGACCATAGCAAGGCTGAATTTGCCCTAGACTTAATTCTGTACTGTGG
  CTGTGAAGGCTGGGAGCCAGAATATGGCGGTTTTACTTCTTACATTGCCAAAGGTGAAGATGAAGAGCTG
  CTAACAGTGAATCCAGAAAGCAATTCTTTGGCATTGGTCTACAGAGACAGAGAGACTCTGAAATTTGTCA
  AGCATATTAACCACCGAAGCCTGGAACAAAAGAAAACCTTCCCAAACAGAACAGGTTTCTGGGACTTTTC
  ATTCATCTATTATGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206839 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNGKRPAEPGPARVGKKGKKEVMAEFSDAVTEETLKKQVAEAWSRRTPFSHEVIVMDMDPFLHCVIPNFI
  QSQDFLEGLQKELMNLDFHEKYNDLYKFQQSDDLKKRREPHISTLRKILFEDFRSWLSDISKIDLESTID
  MSCAKYEFTDALLCHDDELEGRRIAFILYLVPPWDRSMGGTLDLYSIDEHFQPKQIVKSLIPSWNKLVFF
  EVSPVSFHQVSEVLSEEKSRLSISGWFHGPSLTRPPNYFEPPIPRSPHIPQDHEILYDWINPTYLDMDYQ
  VQIQEEFEESSEILLKEFLKPEKFTKVCEALEHGHVEWSSRGPPNKRFYEKAEESKLPEILKECMKLFRS
  EALFLLLSNFTGLKLHFLAPSEEDEMNDKKEAETTDITEEGTSHSPPEPENNQMAISNNSQQSNEQTDPE
  PEENETKKESSVPMCQGELRHWKTGHYTLIHDHSKAEFALDLILYCGCEGWEPEYGGFTSYIAKGEDEEL
  LTVNPESNSLALVYRDRETLKFVKHINHRSLEQKKTFPNRTGFWDFSFIYYE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018233
• ORF Size: 1626 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018233.4
• RefSeq Size: 3020 bp
• RefSeq ORF: 1629 bp
• Locus ID: 55239
• UniProt ID: Q8N543
• Cytogenetics: 16q13
• MW: 63.2 kDa
• Gene Summary: Prolyl 3-hydroxylase that catalyzes 3-hydroxylation of 'Pro-62' of small ribosomal subunit uS12 (RPS23), thereby regulating protein translation termination efficiency. Involved in stress granule formation.[UniProtKB/Swiss-Prot Function]