NEI3 (NEIL3) (NM_018248) Human Tagged ORF Clone

CAT#: RC206838

NEIL3 (Myc-DDK-tagged)-Human nei endonuclease VIII-like 3 (E. coli) (NEIL3)

Product Images

Circular map for RC206838

• 

  Western blot validation of overexpression lysate (Cat# LY413189) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206838 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NEI3
• Synonyms: FGP2; FPG2; hFPG2; hNEI3; NEI3; ZGRF3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206838 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGAAGGACCAGGCTGTACTCTGAATGGAGAGAAGATTCGAGCGCGGGTGCTCCCGGGCCAGGCGG
  TGACCGGCGTGCGGGGAAGCGCTCTGCGGAGTCTGCAGGGCCGCGCCTTGCGGCTCGCAGCCTCCACGGT
  TGTGGTCTCCCCGCAGGCTGCTGCACTGAATAATGATTCCAGCCAGAATGTCTTGAGCCTGTTTAATGGA
  TATGTTTACAGTGGCGTGGAAACTTTGGGGAAGGAGCTCTTTATGTACTTTGGACCAAAAGCTTTACGGA
  TTCATTTCGGAATGAAAGGCTTCATCATGATTAATCCACTTGAGTATAAATATAAAAATGGAGCTTCTCG
  TGTTTTGGAAGTGCAGCTCACCAAAGATTTGATTTGTTTCTTTGACTCATCAGTAGAACTCAGAAACTCA
  ATGGAAAGCCAACAGAGAATAAGAATGATGAAAGAATTAGATGTATGTTCACCTGAATTTAGTTTCTTGA
  GAGCAGAAAGTGAAGTTAAAAAACAGAAAGGCCGGATGCTAGGTGATGTGCTAATGGATCAGAACGTATT
  GCCTGGAGTAGGGAACATCATCAAAAATGAAGCTCTCTTTGACAGTGGTCTCCACCCAGCTGTTAAAGTT
  TGTCAATTAACAGATGAACAGATCCATCACCTCATGAAAATGATACGTGATTTCAGCATTCTCTTTTACA
  GGTGCCGTAAAGCAGGACTTGCTCTCTCTAAACACTATAAGGTTTACAAGCGTCCCAATTGTGGTCAGTG
  CCACTGCAGAATAACTGTGTGCCGCTTTGGGGACAATAACAGAATGACATATTTCTGTCCTCACTGTCAA
  AAAGAAAATCCTCAACATGTTGACATATGCAAGCTACCGACTAGAAATACTATAATCAGTTGGACATCTA
  GCAGGGTGGATCATGTTATGGACTCCGTGGCTCGGAAGTCGGAAGAGCACTGGACCTGTGTGGTGTGTAC
  TTTAATCAATAAGCCCTCTTCTAAGGCATGTGATGCTTGCTTGACCTCAAGGCCTATTGATTCAGTGCTC
  AAGAGTGAAGAAAATTCTACTGTCTTTAGCCACTTAATGAAGTACCCGTGTAATACTTTTGGAAAACCTC
  ATACAGAAGTCAAGATCAACAGGAAAACTGCATTTGGAACTACAACTCTTGTCTTGACTGATTTTAGCAA
  TAAATCCAGTACTTTGGAAAGAAAAACAAAGCAAAACCAGATACTAGATGAGGAGTTTCAAAACTCTCCT
  CCTGCTAGTGTGTGTTTGAATGATATACAGCACCCCTCCAAGAAGACAACAAACGATATAACTCAACTAT
  CCAGCAAAGTAAACATATCACCTACAATCAGTTCAGAATCTAAATTATTTAGTCCAGCACATAAAAAACC
  GAAAACAGCCCACTACTCATCACCAGAGCTTAAAAGCTGCAACCCTGGATATTCTAACAGTGAACTTCAA
  ATTAATATGACAGATGGCCCTCGTACCTTAAATCCTGACAGCCCTCGCTGCAGTAAACACAACCGCCTCT
  GCATTCTCCGAGTTGTGAGGAAGGATGGGGAAAACAAGGGCAGGCAGTTTTATGCCTGTCCTCTACCTAG
  AGAAGCACAATGTGGATTTTTTGAATGGGCAGATTTGTCCTTCCCATTCTGCAACCATGGCAAGCGTTCC
  ACCATGAAAACAGTATTGAAGATTGGACCTAACAATGGAAAGAATTTTTTTGTGTGTCCTCTTGGGAAGG
  AAAAACAATGCAATTTTTTCCAGTGGGCAGAAAATGGGCCAGGAATAAAAATTATTCCTGGATGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206838 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVEGPGCTLNGEKIRARVLPGQAVTGVRGSALRSLQGRALRLAASTVVVSPQAAALNNDSSQNVLSLFNG
  YVYSGVETLGKELFMYFGPKALRIHFGMKGFIMINPLEYKYKNGASRVLEVQLTKDLICFFDSSVELRNS
  MESQQRIRMMKELDVCSPEFSFLRAESEVKKQKGRMLGDVLMDQNVLPGVGNIIKNEALFDSGLHPAVKV
  CQLTDEQIHHLMKMIRDFSILFYRCRKAGLALSKHYKVYKRPNCGQCHCRITVCRFGDNNRMTYFCPHCQ
  KENPQHVDICKLPTRNTIISWTSSRVDHVMDSVARKSEEHWTCVVCTLINKPSSKACDACLTSRPIDSVL
  KSEENSTVFSHLMKYPCNTFGKPHTEVKINRKTAFGTTTLVLTDFSNKSSTLERKTKQNQILDEEFQNSP
  PASVCLNDIQHPSKKTTNDITQLSSKVNISPTISSESKLFSPAHKKPKTAHYSSPELKSCNPGYSNSELQ
  INMTDGPRTLNPDSPRCSKHNRLCILRVVRKDGENKGRQFYACPLPREAQCGFFEWADLSFPFCNHGKRS
  TMKTVLKIGPNNGKNFFVCPLGKEKQCNFFQWAENGPGIKIIPGC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018248
• ORF Size: 1815 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018248.3
• RefSeq Size: 2402 bp
• RefSeq ORF: 1818 bp
• Locus ID: 55247
• UniProt ID: Q8TAT5
• Cytogenetics: 4q34.3
• Domains: Fapy_DNA_glyco, zf-RanBP
• Protein Families: Druggable Genome
• Protein Pathways: Base excision repair
• MW: 68 kDa
• Gene Summary: NEIL3 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al., 2002 [PubMed 12509226]).[supplied by OMIM, Mar 2008]