ZBTB3 (NM_024784) Human Tagged ORF Clone

CAT#: RC206823

ZBTB3 (Myc-DDK-tagged)-Human zinc finger and BTB domain containing 3 (ZBTB3)

Product Images

Circular map for RC206823

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  Western blot validation of overexpression lysate (Cat# LY403029) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206823 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ZBTB3 protein (Cat# TP306823). The protein was produced from HEK293T cells transfected with ZBTB3 cDNA clone (Cat# RC206823) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZBTB3
• Synonyms: FLJ23392
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206823 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTAGGGAGTTCTCGAAGTGGGGCGTAGAGGCATCGCCTGGCAAGGCCTGGGAGCGAAAAAGATCTC
  TACTTCGTGGAGCCGTTGGACGCTACCGGGGAGCCACTGGTGGGGACCTTTTTTGGGCCCCCTTTCCTTC
  GTGGGGTACTATGGAGTTCCCAGAACACAGTCAGCAGCTGCTGCAGAGCCTCCGGGAGCAGCGGTCCCAG
  GGTTTCCTTTGTGACTGCACCGTGATGGTGGGTAGTACCCAGTTCTTGGCCCATCGGGCTGTGCTGGCCT
  CCTGCAGCCCATTCTTCCAGCTTTTCTACAAGGAGCGGGAATTGGACAAGAGGGATCTGGTGTGTATTCA
  CAATGAAATTGTCACAGCCCCAGCCTTTGGGCTGCTTCTGGACTTTATGTATGCTGGCCAGCTGACCCTG
  AGAGGGGATACCCCTGTGGAGGATGTGCTGGCAGCTGCCAGCTACTTGCACATGAATGACATCGTCAAGG
  TGTGTAAGCGGCGGCTTCAAGCCCGGGCCCTGGCAGAGGCAGACAGTACCAAGAAGGAGGAGGAAACCAA
  CTCACAGCTTCCTAGTTTGGAGTTTTTGTCTAGTACTTCCCGTGGCACCCAACCTTCGTTGGCATCTGCT
  GAGACATCAGGCCACTGGGGCAAAGGGGAATGGAAAGGCTCTGCTGCTCCCTCACCTACTGTCCGTCCTC
  CAGATGAGCCACCAATGTCTAGTGGGGCTGACACTACACAGCCTGGCATGGAGGTTGACGCACCACATCT
  GCGGGCACCTCATCCACCAGTGGCTGATGTCTCTCTTGCCAGCCCTAGTAGCTCCACTGAGACCATTCCT
  ACAAACTACTTCTCTTCTGGCATCTCAGCAGTTTCATTGGAGCCACTGCCATCTCTTGATGTGGGTCCTG
  AGAGTCTGAGGGTGGTGGAACCAAAGGATCCTGGAGGACCACTGCAAGGCTTCTATCCCCCAGCCTCAGC
  CCCAACGTCAGCCCCAGCCCCTGTCTCAGCTCCAGTTCCATCCCAGGCTCCAGCCCCAGCTGAAGCTGAG
  CTGGTCCAGGTGAAAGTTGAAGCTATTGTGATCTCTGATGAAGAGACTGATGTGTCAGATGAACAGCCTC
  AGGGTCCTGAGAGAGCTTTCCCATCTGGAGGAGCAGTGTATGGGGCACAGCCCTCCCAGCCAGAGGCTTT
  TGAAGACCCAGGGGCAGCAGGACTGGAGGAGGTGGGGCCAAGTGACCACTTCCTGCCAACAGACCCTCAT
  CTACCCTACCATCTGCTGCCAGGTGCAGGGCAGTATCATCGAGGACTGGTGACCTCACCTCTGCCTGCAC
  CCGCATCCCTGCATGAGCCACTTTACCTGTCTTCTGAGTACGAAGCAGCTCCAGGAAGCTTTGGGGTTTT
  TACTGAGGATGTTCCCACCTGCAAGACATGTGGGAAGACCTTCTCATGCTCTTACACACTACGGCGACAT
  GCCACGGTGCACACACGTGAGCGACCCTATGAGTGCCGCTACTGCCTGCGGAGCTACACGCAGTCAGGGG
  ACCTCTACCGCCACATCCGCAAGGCTCACAATGAGGACCTGGCCAAACGCAGCAAACCAGATCCAGAAGT
  GGGACCCCTTCTAGGGGTGCAGCCTCTCCCTGGCTCCCCAACAGCAGACAGACAGAGCAGCAGTGGTGGA
  GGGCCACCTAAAGATTTTGTATTGGCCCCAAAAACTAACATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206823 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLREFSKWGVEASPGKAWERKRSLLRGAVGRYRGATGGDLFWAPFPSWGTMEFPEHSQQLLQSLREQRSQ
  GFLCDCTVMVGSTQFLAHRAVLASCSPFFQLFYKERELDKRDLVCIHNEIVTAPAFGLLLDFMYAGQLTL
  RGDTPVEDVLAAASYLHMNDIVKVCKRRLQARALAEADSTKKEEETNSQLPSLEFLSSTSRGTQPSLASA
  ETSGHWGKGEWKGSAAPSPTVRPPDEPPMSSGADTTQPGMEVDAPHLRAPHPPVADVSLASPSSSTETIP
  TNYFSSGISAVSLEPLPSLDVGPESLRVVEPKDPGGPLQGFYPPASAPTSAPAPVSAPVPSQAPAPAEAE
  LVQVKVEAIVISDEETDVSDEQPQGPERAFPSGGAVYGAQPSQPEAFEDPGAAGLEEVGPSDHFLPTDPH
  LPYHLLPGAGQYHRGLVTSPLPAPASLHEPLYLSSEYEAAPGSFGVFTEDVPTCKTCGKTFSCSYTLRRH
  ATVHTRERPYECRYCLRSYTQSGDLYRHIRKAHNEDLAKRSKPDPEVGPLLGVQPLPGSPTADRQSSSGG
  GPPKDFVLAPKTNI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024784
• ORF Size: 1722 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024784.4
• RefSeq Size: 2990 bp
• RefSeq ORF: 1725 bp
• Locus ID: 79842
• UniProt ID: Q9H5J0
• Cytogenetics: 11q12.3
• Domains: BTB, zf-C2H2
• Protein Families: Transcription Factors
• MW: 61.8 kDa
• Gene Summary: May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]