CDC6 (NM_001254) Human Tagged ORF Clone
CDC6 (Myc-DDK-tagged)-Human cell division cycle 6 homolog (S. cerevisiae) (CDC6)
|Type||Human Tagged ORF Clone|
|Synonyms||CDC18L; HsCDC6; HsCDC18; MGORS5|
|E. coli Selection||Kanamycin (25 ug/mL)|
|Mammalian Cell Selection||Neomycin|
>RC206814 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC206814 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||1680 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at email@example.com or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||3053 bp|
|RefSeq ORF||1683 bp|
|Protein Pathways||Cell cycle|
|Gene Summary||The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. [provided by RefSeq, Jul 2008]|
|cDNA Clone Resources|
|RC206814L1||Lenti ORF clone of Human cell division cycle 6 homolog (S. cerevisiae) (CDC6), Myc-DDK-tagged||
|RC206814L2||Lenti ORF clone of Human cell division cycle 6 homolog (S. cerevisiae) (CDC6), mGFP tagged||
|RC206814L3||Lenti ORF clone of Human cell division cycle 6 homolog (S. cerevisiae) (CDC6), Myc-DDK-tagged||
|RC206814L4||Lenti ORF clone of Human cell division cycle 6 homolog (S. cerevisiae) (CDC6), mGFP tagged||
|RG206814||CDC6 (GFP-tagged) - Human cell division cycle 6 homolog (S. cerevisiae) (CDC6)||
|SC119336||CDC6 (untagged)-Human cell division cycle 6 homolog (S. cerevisiae) (CDC6)||