TAFA1 (NM_213609) Human Tagged ORF Clone

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SKU
RC206799
FAM19A1 (Myc-DDK-tagged)-Human family with sequence similarity 19 (chemokine (C-C motif)-like), member A1 (FAM19A1)
  $150.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TAFA1
Synonyms FAM19A1; TAFA-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206799 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAATGGTCTCTGCGATGTCCTGGGTCCTGTATTTGTGGATAAGTGCTTGTGCAATGCTACTCTGCC
ATGGATCCCTTCAGCACACTTTCCAGCAGCATCACCTGCACAGACCAGAAGGAGGGACGTGTGAAGTGAT
AGCAGCACACCGATGTTGTAACAAGAATCGCATTGAGGAGCGGTCACAAACAGTAAAGTGTTCCTGTCTA
CCTGGAAAAGTGGCTGGAACAACAAGAAACCGGCCTTCTTGCGTCGATGCCTCCATAGTGATTGGGAAAT
GGTGGTGTGAGATGGAGCCTTGCCTAGAAGGAGAAGAATGTAAGACACTCCCTGACAATTCTGGATGGAT
GTGCGCAACAGGCAACAAAATTAAGACCACGAGAATTCACCCAAGAACC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC206799 protein sequence
Red=Cloning site Green=Tags(s)

MAMVSAMSWVLYLWISACAMLLCHGSLQHTFQQHHLHRPEGGTCEVIAAHRCCNKNRIEERSQTVKCSCL
PGKVAGTTRNRPSCVDASIVIGKWWCEMEPCLEGEECKTLPDNSGWMCATGNKIKTTRIHPRT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_213609
ORF Size 399 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_213609.4
RefSeq Size 2032 bp
RefSeq ORF 402 bp
Locus ID 407738
UniProt ID Q7Z5A9
Cytogenetics 3p14.1
Protein Families Secreted Protein, Transmembrane
MW 14.9 kDa
Summary This gene is a member of the TAFA family which is composed of five highly homologous genes that encode small secreted proteins. These proteins contain conserved cysteine residues at fixed positions, and are distantly related to MIP-1alpha, a member of the CC-chemokine family. The TAFA proteins are predominantly expressed in specific regions of the brain, and are postulated to function as brain-specific chemokines or neurokines that act as regulators of immune and nervous cells. [provided by RefSeq, Jul 2008]
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Documents
Resources
"NM_213609" in other vectors (4)
SKU Description Size Price
RC206799L3 Lenti ORF clone of Human family with sequence similarity 19 (chemokine (C-C motif)-like), member A1 (FAM19A1), Myc-DDK-tagged 10 ug
$450.00
RC206799L4 Lenti ORF clone of Human family with sequence similarity 19 (chemokine (C-C motif)-like), member A1 (FAM19A1), mGFP tagged 10 ug
$450.00
RG206799 FAM19A1 (tGFP-tagged) - Human family with sequence similarity 19 (chemokine (C-C motif)-like), member A1 (FAM19A1) 10 ug
$489.00
SC321974 FAM19A1 (untagged)-Human family with sequence similarity 19 (chemokine (C-C motif)-like), member A1 (FAM19A1) 10 ug
$150.00

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