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CYB5D2 (NM_144611) Human Tagged ORF Clone

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SKU
RC206788
CYB5D2 (Myc-DDK-tagged)-Human cytochrome b5 domain containing 2 (CYB5D2), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Size
Bulk Requests & Clone Modifications

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Other products for "CYB5D2 or NM_144611"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CYB5D2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206788 representing NM_144611
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGAGGTGCGGAGGCCGTGGGCTTTTGTTGGGCCTGGCTGTAGCCGCAGCAGCGGTAATGGCAGCAC
GGCTTATGGGCTGGTGGGGTCCCCGCGCTGGCTTTCGCCTTTTCATACCGGAGGAGCTGTCTCGCTACCG
CGGCGGCCCAGGGGACCCGGGCCTGTACTTGGCGTTGCTCGGCCGTGTCTACGATGTGTCCTCCGGCCGG
AGGCACTACGAGCCTGGGTCCCACTATAGCGGCTTCGCAGGCCGAGACGCATCCAGAGCTTTCGTGACCG
GGGACTGTTCTGAAGCAGGCCTCGTGGATGACGTATCCGACCTGTCAGCCGCTGAGATGCTGACACTTCA
CAATTGGCTTTCATTCTATGAGAAGAATTATGTGTGTGTTGGGAGGGTGACAGGACGGTTCTACGGAGAG
GATGGGCTGCCCACCCCGGCACTGACCCAGGTAGAAGCTGCGATCACCAGAGGCTTGGAGGCCAACAAAC
TACAGCTGCAAGAGAAGCAGACATTCCCGCCGTGCAACGCGGAGTGGAGCTCAGCCAGGGGCAGCCGGCT
CTGGTGCTCCCAGAAGAGTGGAGGTGTGAGCAGAGACTGGATTGGCGTCCCCAGGAAGCTGTATAAGCCA
GGTGCTAAGGAGCCCCGCTGCGTGTGTGTGAGAACCACCGGCCCCCCTAGTGGCCAGATGCCGGACAACC
CTCCACACAGAAATCGTGGGGACCTGGACCACCCAAACTTGGCAGAGTACACAGGCTGCCCACCGCTAGC
CATCACATGCTCCTTTCCACTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC206788 representing NM_144611
Red=Cloning site Green=Tags(s)

MLRCGGRGLLLGLAVAAAAVMAARLMGWWGPRAGFRLFIPEELSRYRGGPGDPGLYLALLGRVYDVSSGR
RHYEPGSHYSGFAGRDASRAFVTGDCSEAGLVDDVSDLSAAEMLTLHNWLSFYEKNYVCVGRVTGRFYGE
DGLPTPALTQVEAAITRGLEANKLQLQEKQTFPPCNAEWSSARGSRLWCSQKSGGVSRDWIGVPRKLYKP
GAKEPRCVCVRTTGPPSGQMPDNPPHRNRGDLDHPNLAEYTGCPPLAITCSFPL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_144611
ORF Size 792 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_144611.4
RefSeq Size 1971 bp
RefSeq ORF 795 bp
Locus ID 124936
UniProt ID Q8WUJ1
Cytogenetics 17p13.2
Domains heme_1
Protein Families Druggable Genome, Transmembrane
MW 28.5 kDa
Summary Heme-binding protein which promotes neuronal but not astrocyte differentiation.[UniProtKB/Swiss-Prot Function]
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Documents
Resources
"NM_144611" in other vectors (5)
SKU Description Size Price
RC206788L3 Lenti ORF clone of Human cytochrome b5 domain containing 2 (CYB5D2), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC206788L4 Lenti ORF clone of Human cytochrome b5 domain containing 2 (CYB5D2), transcript variant 1, mGFP tagged 10 ug
$600.00
RG206788 CYB5D2 (tGFP-tagged) - Human cytochrome b5 domain containing 2 (CYB5D2), transcript variant 1 10 ug
$500.00
SC120753 CYB5D2 (untagged)-Human cytochrome b5 domain containing 2 (CYB5D2), transcript variant 1 10 ug
$300.00
SC324207 CYB5D2 (untagged)-Human cytochrome b5 domain containing 2 (CYB5D2), transcript variant 1 10 ug
$300.00

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