CARD12 (NLRC4) (NM_021209) Human Tagged Lenti ORF Clone

CAT#: RC206757L4

  • LentiORF®

Lenti ORF clone of Human NLR family, CARD domain containing 4 (NLRC4), transcript variant 1, mGFP tagged


  "NM_021209" in other vectors (6)

Reconstitution Protocol

USD 1,191.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (4)
Lenti-vpak packaging kit - packaging plasmids and transfection reagent
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Lenti ORF control particles of pLenti-C-mGFP-P2A-Puro, >1x10^7 TU/ml, 0.5 ml
    • 500 ul

USD 365.00


Forward primer to sequence targets cloned in expression vectors.
    • 1 nmol

USD 71.00


Reverse primer to sequence targets cloned in expression vectors.
    • 1 nmol

USD 71.00

Other products for "CARD12"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag mGFP
Symbol CARD12
Synonyms AIFEC; CARD12; CLAN; CLAN1; CLANA; CLANB; CLANC; CLAND; CLR2.1; FCAS4; IPAF
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
The ORF insert of this clone is exactly the same as(RC206757).
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_021209
ORF Size 3072 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_021209.3
RefSeq Size 3385 bp
RefSeq ORF 3075 bp
Locus ID 58484
UniProt ID Q9NPP4
Cytogenetics 2p22.3
Domains LRR
Protein Pathways NOD-like receptor signaling pathway
MW 116.2 kDa
Gene Summary This gene encodes a member of the caspase recruitment domain-containing NLR family. Family members play essential roles in innate immune response to a wide range of pathogenic organisms, tissue damage and other cellular stresses. Mutations in this gene result in autoinflammation with infantile enterocolitis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.