CARD12 (NLRC4) (NM_021209) Human Tagged ORF Clone

CAT#: RC206757

NLRC4 (Myc-DDK-tagged)-Human NLR family, CARD domain containing 4 (NLRC4), transcript variant 1

Product Images

Circular map for RC206757

• 

  Western blot validation of overexpression lysate (Cat# LY412012) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206757 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CARD12
• Synonyms: AIFEC; CARD12; CLAN; CLAN1; CLANA; CLANB; CLANC; CLAND; CLR2.1; FCAS4; IPAF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206757 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATTTCATAAAGGACAATAGCCGAGCCCTTATTCAAAGAATGGGAATGACTGTTATAAAGCAAATCA
  CAGATGACCTATTTGTATGGAATGTTCTGAATCGCGAAGAAGTAAACATCATTTGCTGCGAGAAGGTGGA
  GCAGGATGCTGCTAGAGGGATCATTCACATGATTTTGAAAAAGGGTTCAGAGTCCTGTAACCTCTTTCTT
  AAATCCCTTAAGGAGTGGAACTATCCTCTATTTCAGGACTTGAATGGACAAAGTCTTTTTCATCAGACAT
  CAGAAGGAGACTTGGACGATTTGGCTCAGGATTTAAAGGACTTGTACCATACCCCATCTTTTCTGAACTT
  TTATCCCCTTGGTGAAGATATTGACATTATTTTTAACTTGAAAAGCACCTTCACAGAACCTGTCCTGTGG
  AGGAAGGACCAACACCATCACCGCGTGGAGCAGCTGACCCTGAATGGCCTCCTGCAGGCTCTTCAGAGCC
  CCTGCATCATTGAAGGGGAATCTGGCAAAGGCAAGTCCACTCTGCTGCAGCGAATTGCCATGCTCTGGGG
  CTCCGGAAAGTGCAAGGCTCTGACCAAGTTCAAATTCGTCTTCTTCCTCCGTCTCAGCAGGGCCCAGGGT
  GGACTTTTTGAAACCCTCTGTGATCAACTCCTGGATATACCTGGCACAATCAGGAAGCAGACATTCATGG
  CCATGCTGCTGAAGCTGCGGCAGAGGGTTCTTTTCCTTCTTGATGGCTACAATGAATTCAAGCCCCAGAA
  CTGCCCAGAAATCGAAGCCCTGATAAAGGAAAACCACCGCTTCAAGAACATGGTCATCGTCACCACTACC
  ACTGAGTGCCTGAGGCACATACGGCAGTTTGGTGCCCTGACTGCTGAGGTGGGGGATATGACAGAAGACA
  GCGCCCAGGCTCTCATCCGAGAAGTGCTGATCAAGGAGCTTGCTGAAGGCTTGTTGCTCCAAATTCAGAA
  ATCCAGGTGCTTGAGGAATCTCATGAAGACCCCTCTCTTTGTGGTCATCACTTGTGCAATCCAGATGGGT
  GAAAGTGAGTTCCACTCTCACACACAAACAACGCTGTTCCATACCTTCTATGATCTGTTGATACAGAAAA
  ACAAACACAAACATAAAGGTGTGGCTGCAAGTGACTTCATTCGGAGCCTGGACCACTGTGGAGACCTAGC
  TCTGGAGGGTGTGTTCTCCCACAAGTTTGATTTCGAACTGCAGGATGTGTCCAGCGTGAATGAGGATGTC
  CTGCTGACAACTGGGCTCCTCTGTAAATATACAGCTCAAAGGTTCAAGCCAAAGTATAAATTCTTTCACA
  AGTCATTCCAGGAGTACACAGCAGGACGAAGACTCAGCAGTTTATTGACGTCTCATGAGCCAGAGGAGGT
  GACCAAGGGGAATGGTTACTTGCAGAAAATGGTTTCCATTTCGGACATTACATCCACTTATAGCAGCCTG
  CTCCGGTACACCTGTGGGTCATCTGTGGAAGCCACCAGGGCTGTTATGAAGCACCTCGCAGCAGTGTATC
  AACACGGCTGCCTTCTCGGACTTTCCATCGCCAAGAGGCCTCTCTGGAGACAGGAATCTTTGCAAAGTGT
  GAAAAACACCACTGAGCAAGAAATTCTGAAAGCCATAAACATCAATTCCTTTGTAGAGTGTGGCATCCAT
  TTATATCAAGAGAGTACATCCAAATCAGCCCTGAGCCAAGAATTTGAAGCTTTCTTTCAAGGTAAAAGCT
  TATATATCAACTCAGGGAACATCCCCGATTACTTATTTGACTTCTTTGAACATTTGCCCAATTGTGCAAG
  TGCCCTGGACTTCATTAAACTGGACTTTTATGGGGGAGCTATGGCTTCATGGGAAAAGGCTGCAGAAGAC
  ACAGGTGGAATCCACATGGAAGAGGCCCCAGAAACCTACATTCCCAGCAGGGCTGTATCTTTGTTCTTCA
  ACTGGAAGCAGGAATTCAGGACTCTGGAGGTCACACTCCGGGATTTCAGCAAGTTGAATAAGCAAGATAT
  CAGATATCTGGGGAAAATATTCAGCTCTGCCACAAGCCTCAGGCTGCAAATAAAGAGATGTGCTGGTGTG
  GCTGGAAGCCTCAGTTTGGTCCTCAGCACCTGTAAGAACATTTATTCTCTCATGGTGGAAGCCAGTCCCC
  TCACCATAGAAGATGAGAGGCACATCACATCTGTAACAAACCTGAAAACCTTGAGTATTCATGACCTACA
  GAATCAACGGCTGCCGGGTGGTCTGACTGACAGCTTGGGTAACTTGAAGAACCTTACAAAGCTCATAATG
  GATAACATAAAGATGAATGAAGAAGATGCTATAAAACTAGCTGAAGGCCTGAAAAACCTGAAGAAGATGT
  GTTTATTTCATTTGACCCACTTGTCTGACATTGGAGAGGGAATGGATTACATAGTCAAGTCTCTGTCAAG
  TGAACCCTGTGACCTTGAAGAAATTCAATTAGTCTCCTGCTGCTTGTCTGCAAATGCAGTGAAAATCCTA
  GCTCAGAATCTTCACAATTTGGTCAAACTGAGCATTCTTGATTTATCAGAAAATTACCTGGAAAAAGATG
  GAAATGAAGCTCTTCATGAACTGATCGACAGGATGAACGTGCTAGAACAGCTCACCGCACTGATGCTGCC
  CTGGGGCTGTGACGTGCAAGGCAGCCTGAGCAGCCTGTTGAAACATTTGGAGGAGGTCCCACAACTCGTC
  AAGCTTGGGTTGAAAAACTGGAGACTCACAGATACAGAGATTAGAATTTTAGGTGCATTTTTTGGAAAGA
  ACCCTCTGAAAAACTTCCAGCAGTTGAATTTGGCGGGAAATCGTGTGAGCAGTGATGGATGGCTTGCCTT
  CATGGGTGTATTTGAGAATCTTAAGCAATTAGTGTTTTTTGACTTTAGTACTAAAGAATTTCTACCTGAT
  CCAGCATTAGTCAGAAAACTTAGCCAAGTGTTATCCAAGTTAACTTTTCTGCAAGAAGCTAGGCTTGTTG
  GGTGGCAATTTGATGATGATGATCTCAGTGTTATTACAGGTGCTTTTAAACTAGTAACTGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206757 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNFIKDNSRALIQRMGMTVIKQITDDLFVWNVLNREEVNIICCEKVEQDAARGIIHMILKKGSESCNLFL
  KSLKEWNYPLFQDLNGQSLFHQTSEGDLDDLAQDLKDLYHTPSFLNFYPLGEDIDIIFNLKSTFTEPVLW
  RKDQHHHRVEQLTLNGLLQALQSPCIIEGESGKGKSTLLQRIAMLWGSGKCKALTKFKFVFFLRLSRAQG
  GLFETLCDQLLDIPGTIRKQTFMAMLLKLRQRVLFLLDGYNEFKPQNCPEIEALIKENHRFKNMVIVTTT
  TECLRHIRQFGALTAEVGDMTEDSAQALIREVLIKELAEGLLLQIQKSRCLRNLMKTPLFVVITCAIQMG
  ESEFHSHTQTTLFHTFYDLLIQKNKHKHKGVAASDFIRSLDHCGDLALEGVFSHKFDFELQDVSSVNEDV
  LLTTGLLCKYTAQRFKPKYKFFHKSFQEYTAGRRLSSLLTSHEPEEVTKGNGYLQKMVSISDITSTYSSL
  LRYTCGSSVEATRAVMKHLAAVYQHGCLLGLSIAKRPLWRQESLQSVKNTTEQEILKAININSFVECGIH
  LYQESTSKSALSQEFEAFFQGKSLYINSGNIPDYLFDFFEHLPNCASALDFIKLDFYGGAMASWEKAAED
  TGGIHMEEAPETYIPSRAVSLFFNWKQEFRTLEVTLRDFSKLNKQDIRYLGKIFSSATSLRLQIKRCAGV
  AGSLSLVLSTCKNIYSLMVEASPLTIEDERHITSVTNLKTLSIHDLQNQRLPGGLTDSLGNLKNLTKLIM
  DNIKMNEEDAIKLAEGLKNLKKMCLFHLTHLSDIGEGMDYIVKSLSSEPCDLEEIQLVSCCLSANAVKIL
  AQNLHNLVKLSILDLSENYLEKDGNEALHELIDRMNVLEQLTALMLPWGCDVQGSLSSLLKHLEEVPQLV
  KLGLKNWRLTDTEIRILGAFFGKNPLKNFQQLNLAGNRVSSDGWLAFMGVFENLKQLVFFDFSTKEFLPD
  PALVRKLSQVLSKLTFLQEARLVGWQFDDDDLSVITGAFKLVTA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021209
• ORF Size: 3072 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021209.4
• RefSeq Size: 3385 bp
• RefSeq ORF: 3075 bp
• Locus ID: 58484
• UniProt ID: Q9NPP4
• Cytogenetics: 2p22.3
• Domains: LRR
• Protein Pathways: NOD-like receptor signaling pathway
• MW: 116.2 kDa
• Gene Summary: This gene encodes a member of the caspase recruitment domain-containing NLR family. Family members play essential roles in innate immune response to a wide range of pathogenic organisms, tissue damage and other cellular stresses. Mutations in this gene result in autoinflammation with infantile enterocolitis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]