CARD12 (NLRC4) (NM_021209) Human Tagged ORF Clone

CAT#: RC206757

Reviews ()
Write a review

NLRC4 (Myc-DDK-tagged)-Human NLR family, CARD domain containing 4 (NLRC4), transcript variant 1



  "NM_021209" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 891.00


Availability*
In Stock

Size
    • 10 ug


Product images

Other products for "NLRC4"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol NLRC4
Synonyms AIFEC; CARD12; CLAN; CLAN1; CLANA; CLANB; CLANC; CLAND; CLR2.1; FCAS4; IPAF
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC206757 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATTTCATAAAGGACAATAGCCGAGCCCTTATTCAAAGAATGGGAATGACTGTTATAAAGCAAATCA
CAGATGACCTATTTGTATGGAATGTTCTGAATCGCGAAGAAGTAAACATCATTTGCTGCGAGAAGGTGGA
GCAGGATGCTGCTAGAGGGATCATTCACATGATTTTGAAAAAGGGTTCAGAGTCCTGTAACCTCTTTCTT
AAATCCCTTAAGGAGTGGAACTATCCTCTATTTCAGGACTTGAATGGACAAAGTCTTTTTCATCAGACAT
CAGAAGGAGACTTGGACGATTTGGCTCAGGATTTAAAGGACTTGTACCATACCCCATCTTTTCTGAACTT
TTATCCCCTTGGTGAAGATATTGACATTATTTTTAACTTGAAAAGCACCTTCACAGAACCTGTCCTGTGG
AGGAAGGACCAACACCATCACCGCGTGGAGCAGCTGACCCTGAATGGCCTCCTGCAGGCTCTTCAGAGCC
CCTGCATCATTGAAGGGGAATCTGGCAAAGGCAAGTCCACTCTGCTGCAGCGAATTGCCATGCTCTGGGG
CTCCGGAAAGTGCAAGGCTCTGACCAAGTTCAAATTCGTCTTCTTCCTCCGTCTCAGCAGGGCCCAGGGT
GGACTTTTTGAAACCCTCTGTGATCAACTCCTGGATATACCTGGCACAATCAGGAAGCAGACATTCATGG
CCATGCTGCTGAAGCTGCGGCAGAGGGTTCTTTTCCTTCTTGATGGCTACAATGAATTCAAGCCCCAGAA
CTGCCCAGAAATCGAAGCCCTGATAAAGGAAAACCACCGCTTCAAGAACATGGTCATCGTCACCACTACC
ACTGAGTGCCTGAGGCACATACGGCAGTTTGGTGCCCTGACTGCTGAGGTGGGGGATATGACAGAAGACA
GCGCCCAGGCTCTCATCCGAGAAGTGCTGATCAAGGAGCTTGCTGAAGGCTTGTTGCTCCAAATTCAGAA
ATCCAGGTGCTTGAGGAATCTCATGAAGACCCCTCTCTTTGTGGTCATCACTTGTGCAATCCAGATGGGT
GAAAGTGAGTTCCACTCTCACACACAAACAACGCTGTTCCATACCTTCTATGATCTGTTGATACAGAAAA
ACAAACACAAACATAAAGGTGTGGCTGCAAGTGACTTCATTCGGAGCCTGGACCACTGTGGAGACCTAGC
TCTGGAGGGTGTGTTCTCCCACAAGTTTGATTTCGAACTGCAGGATGTGTCCAGCGTGAATGAGGATGTC
CTGCTGACAACTGGGCTCCTCTGTAAATATACAGCTCAAAGGTTCAAGCCAAAGTATAAATTCTTTCACA
AGTCATTCCAGGAGTACACAGCAGGACGAAGACTCAGCAGTTTATTGACGTCTCATGAGCCAGAGGAGGT
GACCAAGGGGAATGGTTACTTGCAGAAAATGGTTTCCATTTCGGACATTACATCCACTTATAGCAGCCTG
CTCCGGTACACCTGTGGGTCATCTGTGGAAGCCACCAGGGCTGTTATGAAGCACCTCGCAGCAGTGTATC
AACACGGCTGCCTTCTCGGACTTTCCATCGCCAAGAGGCCTCTCTGGAGACAGGAATCTTTGCAAAGTGT
GAAAAACACCACTGAGCAAGAAATTCTGAAAGCCATAAACATCAATTCCTTTGTAGAGTGTGGCATCCAT
TTATATCAAGAGAGTACATCCAAATCAGCCCTGAGCCAAGAATTTGAAGCTTTCTTTCAAGGTAAAAGCT
TATATATCAACTCAGGGAACATCCCCGATTACTTATTTGACTTCTTTGAACATTTGCCCAATTGTGCAAG
TGCCCTGGACTTCATTAAACTGGACTTTTATGGGGGAGCTATGGCTTCATGGGAAAAGGCTGCAGAAGAC
ACAGGTGGAATCCACATGGAAGAGGCCCCAGAAACCTACATTCCCAGCAGGGCTGTATCTTTGTTCTTCA
ACTGGAAGCAGGAATTCAGGACTCTGGAGGTCACACTCCGGGATTTCAGCAAGTTGAATAAGCAAGATAT
CAGATATCTGGGGAAAATATTCAGCTCTGCCACAAGCCTCAGGCTGCAAATAAAGAGATGTGCTGGTGTG
GCTGGAAGCCTCAGTTTGGTCCTCAGCACCTGTAAGAACATTTATTCTCTCATGGTGGAAGCCAGTCCCC
TCACCATAGAAGATGAGAGGCACATCACATCTGTAACAAACCTGAAAACCTTGAGTATTCATGACCTACA
GAATCAACGGCTGCCGGGTGGTCTGACTGACAGCTTGGGTAACTTGAAGAACCTTACAAAGCTCATAATG
GATAACATAAAGATGAATGAAGAAGATGCTATAAAACTAGCTGAAGGCCTGAAAAACCTGAAGAAGATGT
GTTTATTTCATTTGACCCACTTGTCTGACATTGGAGAGGGAATGGATTACATAGTCAAGTCTCTGTCAAG
TGAACCCTGTGACCTTGAAGAAATTCAATTAGTCTCCTGCTGCTTGTCTGCAAATGCAGTGAAAATCCTA
GCTCAGAATCTTCACAATTTGGTCAAACTGAGCATTCTTGATTTATCAGAAAATTACCTGGAAAAAGATG
GAAATGAAGCTCTTCATGAACTGATCGACAGGATGAACGTGCTAGAACAGCTCACCGCACTGATGCTGCC
CTGGGGCTGTGACGTGCAAGGCAGCCTGAGCAGCCTGTTGAAACATTTGGAGGAGGTCCCACAACTCGTC
AAGCTTGGGTTGAAAAACTGGAGACTCACAGATACAGAGATTAGAATTTTAGGTGCATTTTTTGGAAAGA
ACCCTCTGAAAAACTTCCAGCAGTTGAATTTGGCGGGAAATCGTGTGAGCAGTGATGGATGGCTTGCCTT
CATGGGTGTATTTGAGAATCTTAAGCAATTAGTGTTTTTTGACTTTAGTACTAAAGAATTTCTACCTGAT
CCAGCATTAGTCAGAAAACTTAGCCAAGTGTTATCCAAGTTAACTTTTCTGCAAGAAGCTAGGCTTGTTG
GGTGGCAATTTGATGATGATGATCTCAGTGTTATTACAGGTGCTTTTAAACTAGTAACTGCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC206757 protein sequence
Red=Cloning site Green=Tags(s)

MNFIKDNSRALIQRMGMTVIKQITDDLFVWNVLNREEVNIICCEKVEQDAARGIIHMILKKGSESCNLFL
KSLKEWNYPLFQDLNGQSLFHQTSEGDLDDLAQDLKDLYHTPSFLNFYPLGEDIDIIFNLKSTFTEPVLW
RKDQHHHRVEQLTLNGLLQALQSPCIIEGESGKGKSTLLQRIAMLWGSGKCKALTKFKFVFFLRLSRAQG
GLFETLCDQLLDIPGTIRKQTFMAMLLKLRQRVLFLLDGYNEFKPQNCPEIEALIKENHRFKNMVIVTTT
TECLRHIRQFGALTAEVGDMTEDSAQALIREVLIKELAEGLLLQIQKSRCLRNLMKTPLFVVITCAIQMG
ESEFHSHTQTTLFHTFYDLLIQKNKHKHKGVAASDFIRSLDHCGDLALEGVFSHKFDFELQDVSSVNEDV
LLTTGLLCKYTAQRFKPKYKFFHKSFQEYTAGRRLSSLLTSHEPEEVTKGNGYLQKMVSISDITSTYSSL
LRYTCGSSVEATRAVMKHLAAVYQHGCLLGLSIAKRPLWRQESLQSVKNTTEQEILKAININSFVECGIH
LYQESTSKSALSQEFEAFFQGKSLYINSGNIPDYLFDFFEHLPNCASALDFIKLDFYGGAMASWEKAAED
TGGIHMEEAPETYIPSRAVSLFFNWKQEFRTLEVTLRDFSKLNKQDIRYLGKIFSSATSLRLQIKRCAGV
AGSLSLVLSTCKNIYSLMVEASPLTIEDERHITSVTNLKTLSIHDLQNQRLPGGLTDSLGNLKNLTKLIM
DNIKMNEEDAIKLAEGLKNLKKMCLFHLTHLSDIGEGMDYIVKSLSSEPCDLEEIQLVSCCLSANAVKIL
AQNLHNLVKLSILDLSENYLEKDGNEALHELIDRMNVLEQLTALMLPWGCDVQGSLSSLLKHLEEVPQLV
KLGLKNWRLTDTEIRILGAFFGKNPLKNFQQLNLAGNRVSSDGWLAFMGVFENLKQLVFFDFSTKEFLPD
PALVRKLSQVLSKLTFLQEARLVGWQFDDDDLSVITGAFKLVTA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_021209
ORF Size 3072 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_021209.4
RefSeq Size 3385 bp
RefSeq ORF 3075 bp
Locus ID 58484
UniProt ID Q9NPP4
Cytogenetics 2p22.3
Domains LRR
Protein Pathways NOD-like receptor signaling pathway
MW 116.2 kDa
Gene Summary This gene encodes a member of the caspase recruitment domain-containing NLR family. Family members play essential roles in innate immune response to a wide range of pathogenic organisms, tissue damage and other cellular stresses. Mutations in this gene result in autoinflammation with infantile enterocolitis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

Other Versions

Frequently bought together (4)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 30 ul

USD 177.00


pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

USD 650.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 160.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00

Customer Reviews 
Loading...
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.