ARG2 (NM_001172) Human Tagged ORF Clone
ARG2 (Myc-DDK-tagged)-Human arginase, type II (ARG2), nuclear gene encoding mitochondrial protein
|Product Name||ARG2 (NM_001172) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||arginase, type II; arginase 2; kidney arginase; L-arginine amidinohydrolase; L-arginine ureahydrolase; nonhepatic arginase|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC206756 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC206756 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||1062 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at firstname.lastname@example.org or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_001172.1, NM_001172.2, NM_001172.3, NP_001163.1|
|RefSeq Size||1981 bp|
|RefSeq ORF||1065 bp|
|Protein Pathways||Arginine and proline metabolism, Metabolic pathways|
|Gene Summary||Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exists (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type II isoform encoded by this gene, is located in the mitochondria and expressed in extra-hepatic tissues, especially kidney. The physiologic role of this isoform is poorly understood; it is thought to play a role in nitric oxide and polyamine metabolism. Transcript variants of the type II gene resulting from the use of alternative polyadenylation sites have been described. [provided by RefSeq, Jul 2008]|
|The use of this cDNA Clones has been cited in the following citations:|
Homoarginine and inhibition of human arginase activity: kinetic characterization and biological relevance
,Tommasi, S;Elliot, DJ;Da Boit, M;Gray, SR;Lewis, BC;Mangoni, AA;,
Sci Rep 2018
,PubMed ID 29487337
|cDNA Clone Resources|
|SC125302||ARG2 (untagged)-Human arginase, type II (ARG2), nuclear gene encoding mitochondrial protein||
|SC323830||ARG2 (untagged)-Human arginase, type II (ARG2), nuclear gene encoding mitochondrial protein||
|RG206756||ARG2 (GFP-tagged) - Human arginase, type II (ARG2), nuclear gene encoding mitochondrial protein||
|RC206756L1||Lenti ORF clone of Human arginase, type II (ARG2), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged||
|RC206756L2||Lenti ORF clone of Human arginase, type II (ARG2), nuclear gene encoding mitochondrial protein, mGFP tagged||
|RC206756L3||Lenti ORF clone of Human arginase, type II (ARG2), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged||
|RC206756L4||Lenti ORF clone of Human arginase, type II (ARG2), nuclear gene encoding mitochondrial protein, mGFP tagged||