KASH5 (NM_144688) Human Tagged ORF Clone

CAT#: RC206728

CCDC155 (Myc-DDK-tagged)-Human coiled-coil domain containing 155 (CCDC155)

Product Images

Circular map for RC206728

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  Western blot validation of overexpression lysate (Cat# LY408181) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206728 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CCDC155 protein (Cat# TP306728). The protein was produced from HEK293T cells transfected with CCDC155 cDNA clone (Cat# RC206728) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KASH5
• Synonyms: CCDC155
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206728 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCTGCCTGAGGGCCCGGTGGGTGGCCCCACTGCGGAAATGTACCTCCGGGAGCGGCCTGAGGAGG
  CAAGGCTGGGAATGCCGGTCAGCTTGGAGGAGCAAATACTCAACTCCACGTTCGAAGCTTGTGACCCTCA
  GAGGACAGGCACTGTGGCTGTGGCCCAGGTGCTGGCCTACCTGGAGGCTGTGACAGGCCAGGGCCCCCAG
  GATGCACGCCTCCAAACATTGGCCAACAGCCTGGACCCCAATGGGGAGGGCCCTAAGGCCACTGTGGACT
  TGGACACTTTCCTGGTTGTCATGCGTGACTGGATTGCTGCCTGTCAACTACATGGGGGATTAGAGCTGGA
  AGAGGAGACCGCCTTCCAGGGAGCCCTGACCTCCCAGCAACTGCCATCTGGATGCCCAGAAGCTGAGGAG
  CCAGCCAACCTGGAGAGCTTCGGAGGCGAAGACCCCAGACCCGAGCTACAAGCCACAGCTGACCTGCTGA
  GCAGCCTGGAGGACCTGGAGCTCAGCAACCGACGTCTGGTTGGGGAGAATGCCAAACTGCAGCGGAGCAT
  GGAGACAGCTGAGGAGGGGTCAGCACGCCTTGGGGAGGAGATCTTGGCTCTGCGTAAGCAGCTTCACAGC
  ACCCAGCAGGCCCTGCAGTTTGCCAAGGCCATGGATGAGGAGCTGGAGGACCTGAAGACTCTGGCCAGGA
  GCCTGGAGGAACAGAATCGCAGCCTTCTGGCCCAAGCCCGGCAGGCGGAAAAGGAGCAGCAGCATCTGGT
  GGCTGAGATGGAGACTCTGCAGGAGGAGAACGGGAAGCTGCTTGCCGAGCGGGATGGAGTGAAAAAGAGA
  AGTCAGGAGCTGGCCATGGAGAAGGACACTTTGAAGCGGCAGCTCTTTGAGTGTGAACACCTCATTTGCC
  AAAGAGACACCATCCTCTCTGAGCGCACTCGCGATGTGGAGAGCCTGGCCCAGACCCTGGAAGAATACAG
  AGTGACGACGCAGGAACTGAGGCTGGAGATTTCACGCCTGGAGGAGCAGCTGAGTCAGACCTATGAGGGG
  CCCGATGAGCTACCTGAAGGGGCCCAGCTGAGAAGAGTGGGCTGGACCGAGCTGCTACCCCCATCGCTGG
  GCTTGGAGATCGAGGCCATTCGACAGAAACAGGAAGTGGCAACTGCTGATCTCTCCAACCCTCTGTGTGG
  GGTGTGGCAGTGGGAGGAAGTCATCCATGAGACCAGTGAGGAAACTGAGTTTCCATCTGAAGCCCCAGCT
  GGGGGACAGAGAAACTTCCAGGGAGAGCCAGCGCACCCTGAAGAAGGAAGGAAGGAGCCATCCATGTGGT
  TGACCAGAAGAGAGGAAGAGGAGGATGCAGAGAGCCAGGTCACGGCTGATCTCCCTGTCCCTCTAGGAGC
  CCCTCGCCCTGGAGACATCCCAGAAAACCCTCCAGAGAGACCTGCGCGGCGGGAACTCCAGCAAGCCCTG
  GTGCCTGTGATGAAAAAGCTGGTCCCAGTCAGGAGGAGGGCCTGGGGCCAGCTCTGCCTGCCCCCACAGC
  GGCTCAGAGTCACTCGACATCCACTGATCCCAGCTCCTGTCCTGGGCCTGCTGCTGCTGCTGCTGCTCTC
  TGTCCTGCTGCTTGGCCCGTCCCCACCTCCCACCTGGCCCCACCTCCAGCTCTGCTACCTCCAGCCCCCT
  CCAGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206728 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDLPEGPVGGPTAEMYLRERPEEARLGMPVSLEEQILNSTFEACDPQRTGTVAVAQVLAYLEAVTGQGPQ
  DARLQTLANSLDPNGEGPKATVDLDTFLVVMRDWIAACQLHGGLELEEETAFQGALTSQQLPSGCPEAEE
  PANLESFGGEDPRPELQATADLLSSLEDLELSNRRLVGENAKLQRSMETAEEGSARLGEEILALRKQLHS
  TQQALQFAKAMDEELEDLKTLARSLEEQNRSLLAQARQAEKEQQHLVAEMETLQEENGKLLAERDGVKKR
  SQELAMEKDTLKRQLFECEHLICQRDTILSERTRDVESLAQTLEEYRVTTQELRLEISRLEEQLSQTYEG
  PDELPEGAQLRRVGWTELLPPSLGLEIEAIRQKQEVATADLSNPLCGVWQWEEVIHETSEETEFPSEAPA
  GGQRNFQGEPAHPEEGRKEPSMWLTRREEEEDAESQVTADLPVPLGAPRPGDIPENPPERPARRELQQAL
  VPVMKKLVPVRRRAWGQLCLPPQRLRVTRHPLIPAPVLGLLLLLLLSVLLLGPSPPPTWPHLQLCYLQPP
  PV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144688
• ORF Size: 1686 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144688.5
• RefSeq Size: 2383 bp
• RefSeq ORF: 1689 bp
• Locus ID: 147872
• UniProt ID: Q8N6L0
• Cytogenetics: 19q13.33
• MW: 62.8 kDa
• Gene Summary: As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex, involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Required for telomere attachment to nuclear envelope in the prophase of meiosis and for rapid telomere prophase movements implicating a SUN1/2:KASH5 LINC complex in which SUN1 and SUN2 seem to act at least partial redundantly. Required for homologue pairing during meiotic prophase in spermatocytes and probably oocytes. Essential for male and female gametogenesis. Recruits cytoplasmic dynein to telomere attachment sites at the nuclear envelope in spermatocytes. In oocytes is involved in meiotic resumption and spindle formation.[UniProtKB/Swiss-Prot Function]