Mitochondrial Ferritin (FTMT) (NM_177478) Human Tagged ORF Clone

CAT#: RC206669

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FTMT (Myc-DDK-tagged)-Human ferritin mitochondrial (FTMT), nuclear gene encoding mitochondrial protein



  "NM_177478" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 118.00

USD 429.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol FTMT
Synonyms MTF
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC206669 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGTCCTGCTTCAGGCTCCTCTCCAGGCACATCAGCCCTTCGCTGGCGTCTCTGCGCCCGGTGCGCT
GCTGCTTCGCGCTCCCGCTGCGTTGGGCCCCGGGGCGCCCCTTGGACCCCAGGCAGATCGCCCCCCGCCG
CCCCCTGGCCGCAGCCGCCTCCTCCCGGGACCCTACCGGGCCCGCCGCCGGCCCCTCTCGGGTGCGCCAG
AACTTCCACCCCGACTCCGAGGCTGCCATCAACCGCCAGATCAACCTCGAGCTCTATGCGTCCTACGTGT
ACTTGTCCATGGCCTATTACTTCTCCCGGGATGACGTGGCCTTGAACAACTTCTCCAGGTATTTCCTTCA
CCAGTCCCGGGAGGAGACCGAGCACGCGGAGAAGCTGATGAGGCTGCAGAACCAGCGAGGAGGCCGGATC
CGCCTGCAGGACATCAAGAAGCCGGAACAGGACGACTGGGAAAGCGGGCTGCATGCCATGGAGTGTGCTC
TACTCTTGGAAAAGAACGTGAACCAGTCGTTGCTGGAATTGCACGCTCTAGCCTCAGATAAAGGTGACCC
CCATTTGTGCGATTTCCTGGAAACCTACTACCTGAATGAGCAGGTGAAGTCTATCAAAGAACTAGGTGAC
CACGTGCACAACTTAGTGAAGATGGGGGCCCCGGATGCTGGCCTGGCGGAGTACCTTTTTGACACACATA
CCCTTGGAAATGAAAACAAGCAGAAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC206669 protein sequence
Red=Cloning site Green=Tags(s)

MLSCFRLLSRHISPSLASLRPVRCCFALPLRWAPGRPLDPRQIAPRRPLAAAASSRDPTGPAAGPSRVRQ
NFHPDSEAAINRQINLELYASYVYLSMAYYFSRDDVALNNFSRYFLHQSREETEHAEKLMRLQNQRGGRI
RLQDIKKPEQDDWESGLHAMECALLLEKNVNQSLLELHALASDKGDPHLCDFLETYYLNEQVKSIKELGD
HVHNLVKMGAPDAGLAEYLFDTHTLGNENKQN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_177478
ORF Size 726 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_177478.2
RefSeq Size 894 bp
RefSeq ORF 729 bp
Locus ID 94033
UniProt ID Q8N4E7
Cytogenetics 5q23.1
Protein Pathways Porphyrin and chlorophyll metabolism
MW 27.5 kDa
Gene Summary Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.[UniProtKB/Swiss-Prot Function]

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