C12orf26 (METTL25) (NM_032230) Human Tagged ORF Clone

CAT#: RC206658

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METTL25 (Myc-DDK-tagged)-Human chromosome 12 open reading frame 26 (C12orf26)



Reconstitution Protocol

USD 561.00

In Stock*

Size
    • 10 ug

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Frequently bought together (4)
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol METTL25
Synonyms C12orf26
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC206658 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCTTCTTGCCCTCTCCCGGTGACCCCGGACCTGCCCACGCTGCGTGCCAAGTTGCAGGGACTGC
TGCAGTTCCTGAGGGATGCCCTGTCCATTTCCAATGCACATACCGTGGATTTCTACACAGAATCCGTGTG
GGAGGAGCTGGTCGACTTGCCACCGGAGACAGTGCTGGCTGCGCTGAGGAAGTCAGCGTCGGAGACGGAG
GCCCTGCCCTCAGAGACGCGCCCCCTAGTGGAAGCAGAGTGGGAAGCAGGTATGACTGATTTTCCCAAAA
TATTTTGTGAAACTTCTCAGAAGTTGGTGAGTGTGGAAGCCTTTGCTCTGGCTGCGAAATACTATTCTGT
ACAAAACTTGGGAATATGTACTCCTTTTGAACAGTTGCTTGTAGCCCTTCGAGGAAATCAAAACCAGAGA
ATTGGTGAAAATCAGAAGGCAGTTGAGTTTATGAATATGAAGAAATCTCATGAAGTTCAGGCAATGTCAG
AGCTGATTAGCAGTATTGCTGACTACTATGGAATAAAGCAGGTGATTGACTTGGGTTCCGGTAAAGGCTA
CCTAAGCTCTTTTTTGTCCTTGAAGTATGGCTTAAAAGTTTATGGAATTGATTCTTCAAATACCAATACT
CATGGAGCTGAGGAGAGAAACAGAAAATTGAAGAAACATTGGAAACTCTGTCATGCTCAGTCAAGATTAG
ATGTCAATGGACTAGCATTAAAAATGGCAAAAGAAAGGAAAGTGAAAAATAAAGTTAAAAATAAAGCTGA
TACTGAGGAAGTGTTTAACAACAGTCCTACAAATCAAGAAAAGATGCCTACCTCAGCTATTTTGCCTGAT
TTTTCTGGCTCTGTAATTTCTAATATCAGAAACCAAATGGAAACCCTTCATTCTCAGCCACATCAAGAAG
AAAATTTGTGTTTTGAAAATTCCTTTTCTCTTATAAACCTTTTGCCTATTAATGCTGTAGAGCCTACTTC
TTCACAGCAAATACCCAACAGAGAAACATCTGAAGCCAATAAAGAGAGAAGAAAAATGACATCAAAGTCA
AGTGAATCAAATATATATTCACCTTTAACCTCTTTTATCACTGCTGATTCAGAACTCCATGACATTATTA
AAGATTTGGAGGATTGTTTGATGGTGGGTCTCCACACTTGTGGTGATCTGGCTCCAAATACTTTGCGAAT
ATTTACCTCCAACTCTGAAATCAAGGGAGTTTGCAGTGTGGGTTGTTGCTACCACCTCTTATCTGAAGAA
TTTGAAAACCAGCATAAAGAACGTACTCAGGAAAAGTGGGGATTTCCAATGTGCCACTATTTAAAGGAAG
AGAGATGGTGCTGTGGTCGTAATGCCAGAATGTCAGCATGTTTGGCATTGGAGCGGGTTGCAGCTGGCCA
AGGGCTGCCTACTGAATCACTCTTCTATCGTGCTGTTCTTCAGGATATTATTAAAGATTGTTATGGCATC
ACCAAATGTGATCGGCATGTTGGTAAAATTTATTCCAAATGTTCTTCTTTTCTGGATTATGTCAGACGGT
CTCTAAAGAAGCTTGGATTAGATGAGTCCAAGCTGCCAGAAAAAATTATAATGAACTACTACGAGAAGTA
TAAGCCTCGAATGAATGAGCTGGAAGCTTTTAATATGTTGAAAGTTGTACTGGCTCCCTGTATAGAGACT
TTGATTCTTCTGGATCGACTTTGTTACCTGAAAGAGCAGGAAGATATTGCATGGTCTGCTCTTGTGAAGT
TGTTTGATCCCGTGAAATCTCCCAGATGTTATGCTGTTATTGCCCTGAAGAAGCAGCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC206658 protein sequence
Red=Cloning site Green=Tags(s)

MAASCPLPVTPDLPTLRAKLQGLLQFLRDALSISNAHTVDFYTESVWEELVDLPPETVLAALRKSASETE
ALPSETRPLVEAEWEAGMTDFPKIFCETSQKLVSVEAFALAAKYYSVQNLGICTPFEQLLVALRGNQNQR
IGENQKAVEFMNMKKSHEVQAMSELISSIADYYGIKQVIDLGSGKGYLSSFLSLKYGLKVYGIDSSNTNT
HGAEERNRKLKKHWKLCHAQSRLDVNGLALKMAKERKVKNKVKNKADTEEVFNNSPTNQEKMPTSAILPD
FSGSVISNIRNQMETLHSQPHQEENLCFENSFSLINLLPINAVEPTSSQQIPNRETSEANKERRKMTSKS
SESNIYSPLTSFITADSELHDIIKDLEDCLMVGLHTCGDLAPNTLRIFTSNSEIKGVCSVGCCYHLLSEE
FENQHKERTQEKWGFPMCHYLKEERWCCGRNARMSACLALERVAAGQGLPTESLFYRAVLQDIIKDCYGI
TKCDRHVGKIYSKCSSFLDYVRRSLKKLGLDESKLPEKIIMNYYEKYKPRMNELEAFNMLKVVLAPCIET
LILLDRLCYLKEQEDIAWSALVKLFDPVKSPRCYAVIALKKQQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_032230
ORF Size 1809 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_032230.1, NP_115606.1
RefSeq Size 2105 bp
RefSeq ORF 1812 bp
Locus ID 84190
UniProt ID Q8N6Q8
Cytogenetics 12q21.31
MW 68.2 kDa
Gene Summary Putative methyltransferase.[UniProtKB/Swiss-Prot Function]
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