SIGLEC5 (NM_003830) Human Tagged ORF Clone

CAT#: RC206610

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SIGLEC5 (Myc-DDK-tagged)-Human sialic acid binding Ig-like lectin 5 (SIGLEC5)



  "NM_003830" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 769.00


Availability*
In Stock

Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol SIGLEC5
Synonyms CD33L2; CD170; OB-BP2; OBBP2; SIGLEC-5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC206610 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCCCCTGCTGCTGCTGCCCCTGCTGTGGGGGGGGTCCCTGCAGGAGAAGCCAGTGTACGAGCTGC
AAGTGCAGAAGTCGGTGACGGTGCAGGAGGGCCTGTGCGTCCTTGTGCCCTGCTCCTTCTCTTACCCCTG
GAGATCCTGGTATTCCTCTCCCCCACTCTACGTCTACTGGTTCCGGGACGGGGAGATCCCATACTACGCT
GAGGTTGTGGCCACAAACAACCCAGACAGAAGAGTGAAGCCAGAGACCCAGGGCCGATTCCGCCTCCTTG
GGGATGTCCAGAAGAAGAACTGCTCCCTGAGCATCGGAGATGCCAGAATGGAGGACACGGGAAGCTATTT
CTTCCGCGTGGAGAGAGGAAGGGATGTAAAATATAGCTACCAACAGAATAAGCTGAACTTGGAGGTGACA
GCCCTGATAGAGAAACCCGACATCCACTTTCTGGAGCCTCTGGAGTCCGGCCGCCCCACAAGGCTGAGCT
GCAGCCTTCCAGGATCCTGTGAAGCGGGACCACCTCTCACATTCTCCTGGACGGGGAATGCCCTCAGCCC
CCTGGACCCCGAGACCACCCGCTCCTCGGAGCTCACCCTCACCCCCAGGCCCGAGGACCATGGCACCAAC
CTCACCTGTCAGATGAAACGCCAAGGAGCTCAGGTGACCACGGAGAGAACTGTCCAGCTCAATGTCTCCT
ATGCACCACAGACCATCACCATCTTCAGGAACGGCATAGCCCTAGAGATCCTGCAAAACACCTCATACCT
TCCGGTCCTGGAGGGCCAGGCTCTGCGGCTGCTCTGTGATGCTCCCAGCAACCCCCCTGCACACCTGAGC
TGGTTCCAGGGCTCCCCTGCCCTGAACGCCACCCCCATCTCCAATACCGGGATCTTGGAGCTTCGTCGAG
TAAGGTCTGCAGAAGAAGGAGGCTTCACCTGCCGCGCTCAGCACCCGCTGGGCTTCCTGCAAATTTTTCT
GAATCTCTCAGTTTACTCCCTCCCACAGTTGCTGGGCCCCTCCTGCTCCTGGGAGGCTGAGGGTCTGCAC
TGCAGATGCTCCTTTCGAGCCCGGCCGGCCCCCTCCCTGTGCTGGCGGCTTGAGGAGAAGCCGCTGGAGG
GGAACAGCAGCCAGGGCTCATTCAAGGTCAACTCCAGCTCAGCTGGGCCCTGGGCCAACAGCTCCCTGAT
CCTCCACGGGGGGCTCAGCTCCGACCTCAAAGTCAGCTGCAAGGCCTGGAACATCTATGGGTCCCAGAGC
GGCTCTGTCCTGCTGCTGCAAGGGAGATCGAACCTTGGGACAGGAGTGGTTCCTGCAGCCCTTGGTGGTG
CTGGTGTCATGGCCCTGCTCTGTATCTGTCTGTGCCTCATCTTCTTTTTAATAGTGAAAGCCCGCAGGAA
GCAAGCAGCTGGGAGACCAGAGAAAATGGATGATGAAGACCCCATTATGGGTACCATCACCTCGGGTTCC
AGGAAGAAGCCCTGGCCAGACAGCGCCGGAGATCAAGCATCTCCTCCTGGGGATGCCCCTCCCTTGGAAG
AACAAAAGGAGCTCCATTATGCCTCCCTTAGTTTTTCTGAGATGAAGTCGAGGGAGCCTAAGGACCAGGA
GGCCCCAAGCACCACGGAGTACTCGGAGATCAAGACAAGCAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC206610 protein sequence
Red=Cloning site Green=Tags(s)

MLPLLLLPLLWGGSLQEKPVYELQVQKSVTVQEGLCVLVPCSFSYPWRSWYSSPPLYVYWFRDGEIPYYA
EVVATNNPDRRVKPETQGRFRLLGDVQKKNCSLSIGDARMEDTGSYFFRVERGRDVKYSYQQNKLNLEVT
ALIEKPDIHFLEPLESGRPTRLSCSLPGSCEAGPPLTFSWTGNALSPLDPETTRSSELTLTPRPEDHGTN
LTCQMKRQGAQVTTERTVQLNVSYAPQTITIFRNGIALEILQNTSYLPVLEGQALRLLCDAPSNPPAHLS
WFQGSPALNATPISNTGILELRRVRSAEEGGFTCRAQHPLGFLQIFLNLSVYSLPQLLGPSCSWEAEGLH
CRCSFRARPAPSLCWRLEEKPLEGNSSQGSFKVNSSSAGPWANSSLILHGGLSSDLKVSCKAWNIYGSQS
GSVLLLQGRSNLGTGVVPAALGGAGVMALLCICLCLIFFLIVKARRKQAAGRPEKMDDEDPIMGTITSGS
RKKPWPDSAGDQASPPGDAPPLEEQKELHYASLSFSEMKSREPKDQEAPSTTEYSEIKTSK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003830
ORF Size 1653 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003830.3
RefSeq Size 2523 bp
RefSeq ORF 1656 bp
Locus ID 8778
UniProt ID O15389
Cytogenetics 19q13.41
Domains ig
Protein Families Druggable Genome, Transmembrane
MW 60.7 kDa
Gene Summary This gene encodes a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. These cell surface lectins are characterized by structural motifs in the immunoglobulin (Ig)-like domains and sialic acid recognition sites in the first Ig V set domain. The encoded protein is a member of the CD33-related subset of Siglecs and inhibits the activation of several cell types including monocytes, macrophages and neutrophils. Binding of group B Streptococcus (GBS) to the encoded protein plays a role in GBS immune evasion. [provided by RefSeq, Feb 2012]

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