PDE1B (NM_000924) Human Tagged ORF Clone

CAT#: RC206588

PDE1B (Myc-DDK-tagged)-Human phosphodiesterase 1B, calmodulin-dependent (PDE1B), transcript variant 1

Product Images

Circular map for RC206588

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PDE1B (Cat# RC206588, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE1B(Cat# TA503319). Positive lysates LY400337 (100ug) and LC400337 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY400337) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206588 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PDE1B protein (Cat# TP306588). The protein was produced from HEK293T cells transfected with PDE1B cDNA clone (Cat# RC206588) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PDE1B
• Synonyms: HEL-S-79p; PDE1B1; PDES1B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206588 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGTCCCCCCGCAGTCCTCCGGAGATGCTGGAGGAGTCGGATTGCCCGTCACCCCTGGAGCTGA
  AGTCAGCCCCCAGCAAGAAGATGTGGATTAAGCTTCGGTCTCTGCTGCGCTACATGGTGAAGCAGTTGGA
  GAATGGGGAGATAAACATTGAGGAGCTGAAGAAAAATCTGGAGTACACAGCTTCTCTGCTGGAAGCCGTC
  TACATAGATGAGACACGGCAAATCTTGGACACGGAGGACGAGCTGCAGGAGCTGCGGTCAGATGCCGTGC
  CTTCGGAGGTGCGGGACTGGCTGGCCTCCACCTTCACCCAGCAGGCCCGGGCCAAAGGCCGCCGAGCAGA
  GGAGAAGCCCAAGTTCCGAAGCATTGTGCACGCTGTGCAGGCTGGGATCTTCGTGGAACGGATGTTCCGG
  AGAACATACACCTCTGTGGGCCCCACTTACTCTACTGCGGTTCTCAACTGTCTCAAGAACCTGGATCTCT
  GGTGCTTTGATGTCTTTTCCTTGAACCAGGCAGCAGATGACCATGCCCTGAGGACCATTGTTTTTGAGTT
  GCTGACTCGGCATAACCTCATCAGCCGCTTCAAGATTCCCACTGTGTTTTTGATGAGTTTCCTGGATGCC
  TTGGAGACAGGCTATGGGAAGTACAAGAATCCTTACCACAACCAGATCCACGCAGCCGATGTTACCCAGA
  CAGTCCATTGCTTCTTGCTCCGCACAGGGATGGTGCACTGCCTGTCGGAGATTGAGCTCCTGGCCATCAT
  CTTTGCTGCAGCTATCCATGATTATGAGCACACGGGCACTACCAACAGCTTCCACATCCAGACCAAGTCA
  GAATGTGCCATCGTGTACAATGATCGTTCAGTGCTGGAGAATCACCACATCAGCTCTGTTTTCCGATTGA
  TGCAGGATGATGAGATGAACATTTTCATCAACCTCACCAAGGATGAGTTTGTAGAACTCCGAGCCCTGGT
  CATTGAGATGGTGTTGGCCACAGACATGTCCTGCCATTTCCAGCAAGTGAAGACCATGAAGACAGCCTTG
  CAACAGCTGGAGAGGATTGACAAGCCCAAGGCCCTGTCTCTACTGCTCCATGCTGCTGACATCAGCCACC
  CAACCAAGCAGTGGTTGGTCCACAGCCGTTGGACCAAGGCCCTCATGGAGGAATTCTTCCGTCAGGGTGA
  CAAGGAGGCAGAGTTGGGCCTGCCCTTTTCTCCACTCTGTGACCGCACTTCCACTCTAGTGGCACAGTCT
  CAGATAGGGTTCATCGACTTCATTGTGGAGCCCACATTCTCTGTGCTGACTGACGTGGCAGAGAAGAGTG
  TTCAGCCCCTGGCGGATGAGGACTCCAAGTCTAAAAACCAGCCCAGCTTTCAGTGGCGCCAGCCCTCTCT
  GGATGTGGAAGTGGGAGACCCCAACCCTGATGTGGTCAGCTTTCGTTCCACCTGGGTCAAGCGCATTCAG
  GAGAATAAGCAGAAATGGAAGGAACGGGCAGCAAGTGGCATCACCAACCAGATGTCCATTGACGAGCTGT
  CCCCCTGTGAAGAAGAGGCCCCCCCATCCCCTGCCGAAGATGAACACAACCAGAATGGGAATCTGGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206588 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MELSPRSPPEMLEESDCPSPLELKSAPSKKMWIKLRSLLRYMVKQLENGEINIEELKKNLEYTASLLEAV
  YIDETRQILDTEDELQELRSDAVPSEVRDWLASTFTQQARAKGRRAEEKPKFRSIVHAVQAGIFVERMFR
  RTYTSVGPTYSTAVLNCLKNLDLWCFDVFSLNQAADDHALRTIVFELLTRHNLISRFKIPTVFLMSFLDA
  LETGYGKYKNPYHNQIHAADVTQTVHCFLLRTGMVHCLSEIELLAIIFAAAIHDYEHTGTTNSFHIQTKS
  ECAIVYNDRSVLENHHISSVFRLMQDDEMNIFINLTKDEFVELRALVIEMVLATDMSCHFQQVKTMKTAL
  QQLERIDKPKALSLLLHAADISHPTKQWLVHSRWTKALMEEFFRQGDKEAELGLPFSPLCDRTSTLVAQS
  QIGFIDFIVEPTFSVLTDVAEKSVQPLADEDSKSKNQPSFQWRQPSLDVEVGDPNPDVVSFRSTWVKRIQ
  ENKQKWKERAASGITNQMSIDELSPCEEEAPPSPAEDEHNQNGNLD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000924
• ORF Size: 1608 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000924.4
• RefSeq Size: 3463 bp
• RefSeq ORF: 1611 bp
• Locus ID: 5153
• UniProt ID: Q01064
• Cytogenetics: 12q13.2
• Domains: PDEase, HDc
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Progesterone-mediated oocyte maturation, Purine metabolism
• MW: 61.4 kDa
• Gene Summary: The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE1 subfamily. Members of the PDE1 family are calmodulin-dependent PDEs that are stimulated by a calcium-calmodulin complex. This PDE has dual-specificity for the second messengers, cAMP and cGMP, with a preference for cGMP as a substrate. cAMP and cGMP function as key regulators of many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]