PDE1B (NM_000924) Human Tagged ORF Clone

CAT#: RC206588

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PDE1B (Myc-DDK-tagged)-Human phosphodiesterase 1B, calmodulin-dependent (PDE1B), transcript variant 1



  "NM_000924" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 499.00


Availability*
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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PDE1B
Synonyms HEL-S-79p; PDE1B1; PDES1B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC206588 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCTGTCCCCCCGCAGTCCTCCGGAGATGCTGGAGGAGTCGGATTGCCCGTCACCCCTGGAGCTGA
AGTCAGCCCCCAGCAAGAAGATGTGGATTAAGCTTCGGTCTCTGCTGCGCTACATGGTGAAGCAGTTGGA
GAATGGGGAGATAAACATTGAGGAGCTGAAGAAAAATCTGGAGTACACAGCTTCTCTGCTGGAAGCCGTC
TACATAGATGAGACACGGCAAATCTTGGACACGGAGGACGAGCTGCAGGAGCTGCGGTCAGATGCCGTGC
CTTCGGAGGTGCGGGACTGGCTGGCCTCCACCTTCACCCAGCAGGCCCGGGCCAAAGGCCGCCGAGCAGA
GGAGAAGCCCAAGTTCCGAAGCATTGTGCACGCTGTGCAGGCTGGGATCTTCGTGGAACGGATGTTCCGG
AGAACATACACCTCTGTGGGCCCCACTTACTCTACTGCGGTTCTCAACTGTCTCAAGAACCTGGATCTCT
GGTGCTTTGATGTCTTTTCCTTGAACCAGGCAGCAGATGACCATGCCCTGAGGACCATTGTTTTTGAGTT
GCTGACTCGGCATAACCTCATCAGCCGCTTCAAGATTCCCACTGTGTTTTTGATGAGTTTCCTGGATGCC
TTGGAGACAGGCTATGGGAAGTACAAGAATCCTTACCACAACCAGATCCACGCAGCCGATGTTACCCAGA
CAGTCCATTGCTTCTTGCTCCGCACAGGGATGGTGCACTGCCTGTCGGAGATTGAGCTCCTGGCCATCAT
CTTTGCTGCAGCTATCCATGATTATGAGCACACGGGCACTACCAACAGCTTCCACATCCAGACCAAGTCA
GAATGTGCCATCGTGTACAATGATCGTTCAGTGCTGGAGAATCACCACATCAGCTCTGTTTTCCGATTGA
TGCAGGATGATGAGATGAACATTTTCATCAACCTCACCAAGGATGAGTTTGTAGAACTCCGAGCCCTGGT
CATTGAGATGGTGTTGGCCACAGACATGTCCTGCCATTTCCAGCAAGTGAAGACCATGAAGACAGCCTTG
CAACAGCTGGAGAGGATTGACAAGCCCAAGGCCCTGTCTCTACTGCTCCATGCTGCTGACATCAGCCACC
CAACCAAGCAGTGGTTGGTCCACAGCCGTTGGACCAAGGCCCTCATGGAGGAATTCTTCCGTCAGGGTGA
CAAGGAGGCAGAGTTGGGCCTGCCCTTTTCTCCACTCTGTGACCGCACTTCCACTCTAGTGGCACAGTCT
CAGATAGGGTTCATCGACTTCATTGTGGAGCCCACATTCTCTGTGCTGACTGACGTGGCAGAGAAGAGTG
TTCAGCCCCTGGCGGATGAGGACTCCAAGTCTAAAAACCAGCCCAGCTTTCAGTGGCGCCAGCCCTCTCT
GGATGTGGAAGTGGGAGACCCCAACCCTGATGTGGTCAGCTTTCGTTCCACCTGGGTCAAGCGCATTCAG
GAGAATAAGCAGAAATGGAAGGAACGGGCAGCAAGTGGCATCACCAACCAGATGTCCATTGACGAGCTGT
CCCCCTGTGAAGAAGAGGCCCCCCCATCCCCTGCCGAAGATGAACACAACCAGAATGGGAATCTGGAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC206588 protein sequence
Red=Cloning site Green=Tags(s)

MELSPRSPPEMLEESDCPSPLELKSAPSKKMWIKLRSLLRYMVKQLENGEINIEELKKNLEYTASLLEAV
YIDETRQILDTEDELQELRSDAVPSEVRDWLASTFTQQARAKGRRAEEKPKFRSIVHAVQAGIFVERMFR
RTYTSVGPTYSTAVLNCLKNLDLWCFDVFSLNQAADDHALRTIVFELLTRHNLISRFKIPTVFLMSFLDA
LETGYGKYKNPYHNQIHAADVTQTVHCFLLRTGMVHCLSEIELLAIIFAAAIHDYEHTGTTNSFHIQTKS
ECAIVYNDRSVLENHHISSVFRLMQDDEMNIFINLTKDEFVELRALVIEMVLATDMSCHFQQVKTMKTAL
QQLERIDKPKALSLLLHAADISHPTKQWLVHSRWTKALMEEFFRQGDKEAELGLPFSPLCDRTSTLVAQS
QIGFIDFIVEPTFSVLTDVAEKSVQPLADEDSKSKNQPSFQWRQPSLDVEVGDPNPDVVSFRSTWVKRIQ
ENKQKWKERAASGITNQMSIDELSPCEEEAPPSPAEDEHNQNGNLD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_000924
ORF Size 1608 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000924.4
RefSeq Size 3463 bp
RefSeq ORF 1611 bp
Locus ID 5153
UniProt ID Q01064
Cytogenetics 12q13.2
Domains PDEase, HDc
Protein Families Druggable Genome
Protein Pathways Calcium signaling pathway, Progesterone-mediated oocyte maturation, Purine metabolism
MW 61.4 kDa
Gene Summary The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE1 subfamily. Members of the PDE1 family are calmodulin-dependent PDEs that are stimulated by a calcium-calmodulin complex. This PDE has dual-specificity for the second messengers, cAMP and cGMP, with a preference for cGMP as a substrate. cAMP and cGMP function as key regulators of many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]

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