SIAH1 (NM_003031) Human Tagged ORF Clone
SIAH1 (Myc-DDK-tagged)-Human seven in absentia homolog 1 (Drosophila) (SIAH1), transcript variant 1
|Type||Human Tagged ORF Clone|
|E. coli Selection||Kanamycin (25 ug/mL)|
|Mammalian Cell Selection||Neomycin|
>RC206576 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC206576 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||939 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at firstname.lastname@example.org or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||2003 bp|
|RefSeq ORF||849 bp|
|Protein Families||Druggable Genome|
|Protein Pathways||p53 signaling pathway, Ubiquitin mediated proteolysis, Wnt signaling pathway|
|Gene Summary||This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]|
|cDNA Clone Resources|
|RC206576L1||Lenti ORF clone of Human seven in absentia homolog 1 (Drosophila) (SIAH1), transcript variant 1, Myc-DDK-tagged||
|RC206576L2||Lenti ORF clone of Human seven in absentia homolog 1 (Drosophila) (SIAH1), transcript variant 1, mGFP tagged||
|RC206576L3||Lenti ORF clone of Human seven in absentia homolog 1 (Drosophila) (SIAH1), transcript variant 1, Myc-DDK-tagged||
|RC206576L4||Lenti ORF clone of Human seven in absentia homolog 1 (Drosophila) (SIAH1), transcript variant 1, mGFP tagged||
|RG206576||SIAH1 (tGFP-tagged) - Human seven in absentia homolog 1 (Drosophila) (SIAH1), transcript variant 1||
|SC118259||SIAH1 (untagged)-Human seven in absentia homolog 1 (Drosophila) (SIAH1), transcript variant 1||
|SC323995||SIAH1 (untagged)-Human seven in absentia homolog 1 (Drosophila) (SIAH1), transcript variant 1||