SLC26A3 (NM_000111) Human Tagged ORF Clone

CAT#: RC206559

SLC26A3 (Myc-DDK-tagged)-Human solute carrier family 26, member 3 (SLC26A3)

Product Images

Western blot validation of overexpression lysate (Cat# LY400033) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206559 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC26A3
• Synonyms: CLD; DRA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206559 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTGAACCCTTTGGGAATCAGTATATTGTGGCCAGGCCAGTGTATTCTACAAATGCTTTTGAGGAAA
  ATCATAAAAAGACAGGAAGACATCATAAGACATTTCTGGATCATCTCAAAGTGTGTTGTAGCTGTTCCCC
  ACAAAAGGCCAAGAGAATTGTCCTCTCTTTGTTCCCCATAGCATCTTGGTTGCCAGCATACCGGCTTAAA
  GAATGGTTGCTCAGTGATATTGTTTCTGGTATCAGCACAGGGATTGTGGCCGTACTACAAGGTTTAGCAT
  TTGCTCTGCTGGTCGACATTCCCCCAGTCTATGGGTTGTATGCATCCTTTTTCCCAGCCATAATCTACCT
  TTTCTTCGGCACTTCCAGACACATATCCGTGGGTCCGTTTCCGATTCTGAGTATGATGGTGGGACTAGCA
  GTTTCAGGAGCAGTTTCAAAAGCAGTCCCAGATCGCAATGCAACTACTTTGGGATTGCCTAACAACTCGA
  ATAATTCTTCACTACTGGATGACGAGAGGGTGAGGGTGGCGGCGGCGGCATCAGTCACAGTGCTTTCTGG
  AATCATCCAGTTGGCTTTTGGGATTCTGCGGATTGGATTTGTAGTGATATACCTGTCTGAGTCCCTCATC
  AGTGGCTTCACTACTGCTGCTGCTGTTCATGTTTTGGTTTCCCAACTCAAATTCATTTTTCAGTTGACAG
  TCCCGTCACACACTGATCCAGTTTCAATTTTCAAAGTACTATACTCTGTATTCTCACAAATAGAGAAGAC
  TAATATTGCAGACCTGGTGACAGCTCTGATTGTCCTTTTGGTTGTATCCATTGTTAAAGAAATAAATCAG
  CGCTTCAAAGACAAACTTCCAGTGCCCATTCCAATCGAATTCATTATGACCGTGATTGCAGCAGGTGTAT
  CCTACGGCTGTGACTTTAAAAACAGGTTTAAAGTGGCTGTGGTTGGGGACATGAATCCTGGATTTCAGCC
  CCCTATTACACCTGACGTGGAGACTTTCCAAAACACCGTAGGAGATTGCTTCGGCATCGCAATGGTTGCA
  TTTGCAGTGGCCTTTTCAGTTGCCAGCGTCTATTCCCTCAAATACGATTATCCACTTGATGGCAATCAGG
  AGTTAATAGCCTTGGGACTGGGTAACATAGTCTGTGGAGTATTCAGAGGATTTGCTGGGAGTACTGCCCT
  CTCCAGATCAGCAGTTCAGGAGAGCACAGGAGGCAAAACACAGATTGCTGGGCTTATTGGTGCCATCATC
  GTGCTGATTGTCGTTCTAGCCATTGGATTTCTCCTGGCGCCTCTACAAAAGTCCGTCCTGGCAGCTTTAG
  CATTGGGAAACTTAAAGGGAATGCTGATGCAGTTTGCTGAAATAGGCAGATTGTGGCGAAAGGACAAATA
  TGATTGTTTAATTTGGATCATGACCTTCATCTTCACCATTGTCCTGGGACTCGGGTTAGGCCTGGCAGCT
  AGTGTGGCATTTCAACTGCTAACCATCGTGTTCAGGACCCAATTTCCAAAATGCAGCACGCTGGCTAATA
  TTGGAAGAACCAACATCTATAAGAATAAAAAAGATTATTATGATATGTATGAGCCAGAAGGAGTGAAAAT
  TTTCAGATGTCCATCTCCTATCTACTTTGCAAACATTGGTTTCTTTAGGCGGAAACTTATCGATGCTGTT
  GGCTTTAGTCCACTTCGAATTCTACGCAAGCGCAACAAAGCTTTGAGGAAAATCCGAAAACTGCAGAAGC
  AAGGCTTGCTACAAGTGACACCAAAAGGATTTATATGTACTGTTGACACCATAAAAGATTCTGACGAAGA
  GCTGGACAACAATCAGATAGAAGTACTGGACCAGCCAATCAATACCACAGACCTGCCTTTCCACATTGAC
  TGGAATGATGATCTTCCTCTCAACATTGAGGTCCCCAAAATCAGCCTCCACAGCCTCATTCTCGACTTTT
  CAGCAGTGTCCTTTCTTGATGTTTCTTCAGTGAGGGGCCTTAAATCGATTTTGCAAGAATTTATCAGGAT
  CAAGGTAGATGTGTATATCGTTGGAACTGATGATGACTTCATTGAGAAGCTTAACCGGTATGAATTTTTT
  GATGGTGAAGTGAAAAGCTCAATATTTTTCTTAACAATCCATGATGCTGTTTTGCATATTTTGATGAAGA
  AAGATTACAGTACTTCAAAGTTTAATCCCAGTCAGGAAAAAGATGGAAAAATTGATTTTACCATAAATAC
  AAATGGAGGATTACGTAATCGGGTATATGAGGTGCCAGTTGAAACAAAATTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206559 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MIEPFGNQYIVARPVYSTNAFEENHKKTGRHHKTFLDHLKVCCSCSPQKAKRIVLSLFPIASWLPAYRLK
  EWLLSDIVSGISTGIVAVLQGLAFALLVDIPPVYGLYASFFPAIIYLFFGTSRHISVGPFPILSMMVGLA
  VSGAVSKAVPDRNATTLGLPNNSNNSSLLDDERVRVAAAASVTVLSGIIQLAFGILRIGFVVIYLSESLI
  SGFTTAAAVHVLVSQLKFIFQLTVPSHTDPVSIFKVLYSVFSQIEKTNIADLVTALIVLLVVSIVKEINQ
  RFKDKLPVPIPIEFIMTVIAAGVSYGCDFKNRFKVAVVGDMNPGFQPPITPDVETFQNTVGDCFGIAMVA
  FAVAFSVASVYSLKYDYPLDGNQELIALGLGNIVCGVFRGFAGSTALSRSAVQESTGGKTQIAGLIGAII
  VLIVVLAIGFLLAPLQKSVLAALALGNLKGMLMQFAEIGRLWRKDKYDCLIWIMTFIFTIVLGLGLGLAA
  SVAFQLLTIVFRTQFPKCSTLANIGRTNIYKNKKDYYDMYEPEGVKIFRCPSPIYFANIGFFRRKLIDAV
  GFSPLRILRKRNKALRKIRKLQKQGLLQVTPKGFICTVDTIKDSDEELDNNQIEVLDQPINTTDLPFHID
  WNDDLPLNIEVPKISLHSLILDFSAVSFLDVSSVRGLKSILQEFIRIKVDVYIVGTDDDFIEKLNRYEFF
  DGEVKSSIFFLTIHDAVLHILMKKDYSTSKFNPSQEKDGKIDFTINTNGGLRNRVYEVPVETKF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000111
• ORF Size: 2292 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000111.1, NP_000102.1
• RefSeq Size: 2894 bp
• RefSeq ORF: 2295 bp
• Locus ID: 1811
• UniProt ID: P40879
• Cytogenetics: 7q22.3-q31.1
• Protein Families: Druggable Genome, Transcription Factors, Transmembrane
• MW: 84.5 kDa
• Gene Summary: The protein encoded by this gene is a transmembrane glycoprotein that transports chloride ions across the cell membrane in exchange for bicarbonate ions. It is localized to the mucosa of the lower intestinal tract, particularly to the apical membrane of columnar epithelium and some goblet cells. The protein is essential for intestinal chloride absorption, and mutations in this gene have been associated with congenital chloride diarrhea. [provided by RefSeq, Oct 2008]