SLC26A3 (NM_000111) Human Tagged ORF Clone

CAT#: RC206559

SLC26A3 (Myc-DDK-tagged)-Human solute carrier family 26, member 3 (SLC26A3)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro


  "NM_000111" in other vectors (6)

Reconstitution Protocol

USD 700.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
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Other products for "SLC26A3"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol SLC26A3
Synonyms CLD; DRA
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC206559 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATTGAACCCTTTGGGAATCAGTATATTGTGGCCAGGCCAGTGTATTCTACAAATGCTTTTGAGGAAA
ATCATAAAAAGACAGGAAGACATCATAAGACATTTCTGGATCATCTCAAAGTGTGTTGTAGCTGTTCCCC
ACAAAAGGCCAAGAGAATTGTCCTCTCTTTGTTCCCCATAGCATCTTGGTTGCCAGCATACCGGCTTAAA
GAATGGTTGCTCAGTGATATTGTTTCTGGTATCAGCACAGGGATTGTGGCCGTACTACAAGGTTTAGCAT
TTGCTCTGCTGGTCGACATTCCCCCAGTCTATGGGTTGTATGCATCCTTTTTCCCAGCCATAATCTACCT
TTTCTTCGGCACTTCCAGACACATATCCGTGGGTCCGTTTCCGATTCTGAGTATGATGGTGGGACTAGCA
GTTTCAGGAGCAGTTTCAAAAGCAGTCCCAGATCGCAATGCAACTACTTTGGGATTGCCTAACAACTCGA
ATAATTCTTCACTACTGGATGACGAGAGGGTGAGGGTGGCGGCGGCGGCATCAGTCACAGTGCTTTCTGG
AATCATCCAGTTGGCTTTTGGGATTCTGCGGATTGGATTTGTAGTGATATACCTGTCTGAGTCCCTCATC
AGTGGCTTCACTACTGCTGCTGCTGTTCATGTTTTGGTTTCCCAACTCAAATTCATTTTTCAGTTGACAG
TCCCGTCACACACTGATCCAGTTTCAATTTTCAAAGTACTATACTCTGTATTCTCACAAATAGAGAAGAC
TAATATTGCAGACCTGGTGACAGCTCTGATTGTCCTTTTGGTTGTATCCATTGTTAAAGAAATAAATCAG
CGCTTCAAAGACAAACTTCCAGTGCCCATTCCAATCGAATTCATTATGACCGTGATTGCAGCAGGTGTAT
CCTACGGCTGTGACTTTAAAAACAGGTTTAAAGTGGCTGTGGTTGGGGACATGAATCCTGGATTTCAGCC
CCCTATTACACCTGACGTGGAGACTTTCCAAAACACCGTAGGAGATTGCTTCGGCATCGCAATGGTTGCA
TTTGCAGTGGCCTTTTCAGTTGCCAGCGTCTATTCCCTCAAATACGATTATCCACTTGATGGCAATCAGG
AGTTAATAGCCTTGGGACTGGGTAACATAGTCTGTGGAGTATTCAGAGGATTTGCTGGGAGTACTGCCCT
CTCCAGATCAGCAGTTCAGGAGAGCACAGGAGGCAAAACACAGATTGCTGGGCTTATTGGTGCCATCATC
GTGCTGATTGTCGTTCTAGCCATTGGATTTCTCCTGGCGCCTCTACAAAAGTCCGTCCTGGCAGCTTTAG
CATTGGGAAACTTAAAGGGAATGCTGATGCAGTTTGCTGAAATAGGCAGATTGTGGCGAAAGGACAAATA
TGATTGTTTAATTTGGATCATGACCTTCATCTTCACCATTGTCCTGGGACTCGGGTTAGGCCTGGCAGCT
AGTGTGGCATTTCAACTGCTAACCATCGTGTTCAGGACCCAATTTCCAAAATGCAGCACGCTGGCTAATA
TTGGAAGAACCAACATCTATAAGAATAAAAAAGATTATTATGATATGTATGAGCCAGAAGGAGTGAAAAT
TTTCAGATGTCCATCTCCTATCTACTTTGCAAACATTGGTTTCTTTAGGCGGAAACTTATCGATGCTGTT
GGCTTTAGTCCACTTCGAATTCTACGCAAGCGCAACAAAGCTTTGAGGAAAATCCGAAAACTGCAGAAGC
AAGGCTTGCTACAAGTGACACCAAAAGGATTTATATGTACTGTTGACACCATAAAAGATTCTGACGAAGA
GCTGGACAACAATCAGATAGAAGTACTGGACCAGCCAATCAATACCACAGACCTGCCTTTCCACATTGAC
TGGAATGATGATCTTCCTCTCAACATTGAGGTCCCCAAAATCAGCCTCCACAGCCTCATTCTCGACTTTT
CAGCAGTGTCCTTTCTTGATGTTTCTTCAGTGAGGGGCCTTAAATCGATTTTGCAAGAATTTATCAGGAT
CAAGGTAGATGTGTATATCGTTGGAACTGATGATGACTTCATTGAGAAGCTTAACCGGTATGAATTTTTT
GATGGTGAAGTGAAAAGCTCAATATTTTTCTTAACAATCCATGATGCTGTTTTGCATATTTTGATGAAGA
AAGATTACAGTACTTCAAAGTTTAATCCCAGTCAGGAAAAAGATGGAAAAATTGATTTTACCATAAATAC
AAATGGAGGATTACGTAATCGGGTATATGAGGTGCCAGTTGAAACAAAATTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC206559 protein sequence
Red=Cloning site Green=Tags(s)

MIEPFGNQYIVARPVYSTNAFEENHKKTGRHHKTFLDHLKVCCSCSPQKAKRIVLSLFPIASWLPAYRLK
EWLLSDIVSGISTGIVAVLQGLAFALLVDIPPVYGLYASFFPAIIYLFFGTSRHISVGPFPILSMMVGLA
VSGAVSKAVPDRNATTLGLPNNSNNSSLLDDERVRVAAAASVTVLSGIIQLAFGILRIGFVVIYLSESLI
SGFTTAAAVHVLVSQLKFIFQLTVPSHTDPVSIFKVLYSVFSQIEKTNIADLVTALIVLLVVSIVKEINQ
RFKDKLPVPIPIEFIMTVIAAGVSYGCDFKNRFKVAVVGDMNPGFQPPITPDVETFQNTVGDCFGIAMVA
FAVAFSVASVYSLKYDYPLDGNQELIALGLGNIVCGVFRGFAGSTALSRSAVQESTGGKTQIAGLIGAII
VLIVVLAIGFLLAPLQKSVLAALALGNLKGMLMQFAEIGRLWRKDKYDCLIWIMTFIFTIVLGLGLGLAA
SVAFQLLTIVFRTQFPKCSTLANIGRTNIYKNKKDYYDMYEPEGVKIFRCPSPIYFANIGFFRRKLIDAV
GFSPLRILRKRNKALRKIRKLQKQGLLQVTPKGFICTVDTIKDSDEELDNNQIEVLDQPINTTDLPFHID
WNDDLPLNIEVPKISLHSLILDFSAVSFLDVSSVRGLKSILQEFIRIKVDVYIVGTDDDFIEKLNRYEFF
DGEVKSSIFFLTIHDAVLHILMKKDYSTSKFNPSQEKDGKIDFTINTNGGLRNRVYEVPVETKF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_000111
ORF Size 2292 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000111.1, NP_000102.1
RefSeq Size 2894 bp
RefSeq ORF 2295 bp
Locus ID 1811
UniProt ID P40879
Cytogenetics 7q22.3-q31.1
Protein Families Druggable Genome, Transcription Factors, Transmembrane
MW 84.5 kDa
Gene Summary The protein encoded by this gene is a transmembrane glycoprotein that transports chloride ions across the cell membrane in exchange for bicarbonate ions. It is localized to the mucosa of the lower intestinal tract, particularly to the apical membrane of columnar epithelium and some goblet cells. The protein is essential for intestinal chloride absorption, and mutations in this gene have been associated with congenital chloride diarrhea. [provided by RefSeq, Oct 2008]

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