HCAR2 (NM_177551) Human Tagged ORF Clone

SKU
RC206527
HCAR2 (Myc-DDK-tagged)-Human G protein-coupled receptor 109A (GPR109A)
  • TrueORF Gold

    Protein expression verified by Western blot, fully sequenced and in stock

    Click here to learn more.

$457.00
In Stock*
Specifications
Product Data
Target Symbol HCAR2
Synonyms GPR109A; HCA2; HM74a; HM74b; NIACR1; Puma-g; PUMAG
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206527 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATCGGCACCATCTGCAGGATCACTTTCTGGAAATAGACAAGAAGAACTGCTGCGTGTTCCGAGATG
ACTTCATTGTCAAGGTGTTGCCGCCGGTGTTGGGGCTGGAGTTTATCTTCGGGCTTCTGGGCAATGGCCT
TGCCCTGTGGATTTTCTGTTTCCACCTCAAGTCCTGGAAATCCAGCCGGATTTTCCTGTTCAACCTGGCA
GTGGCTGACTTTCTACTGATCATCTGCCTGCCCTTCCTGATGGACAACTATGTGAGGCGTTGGGACTGGA
AGTTTGGGGACATCCCTTGCCGGCTGATGCTCTTCATGTTGGCTATGAACCGCCAGGGCAGCATCATCTT
CCTCACGGTGGTGGCGGTAGACAGGTATTTCCGGGTGGTCCATCCCCACCACGCCCTGAACAAGATCTCC
AATCGGACAGCAGCCATCATCTCTTGCCTTCTGTGGGGCATCACTATTGGCCTGACAGTCCACCTCCTGA
AGAAGAAGATGCCGATCCAGAATGGCGGTGCAAATTTGTGCAGCAGCTTCAGCATCTGCCATACCTTCCA
GTGGCACGAAGCCATGTTCCTCCTGGAGTTCTTCCTGCCCCTGGGCATCATCCTGTTCTGCTCAGCCAGA
ATTATCTGGAGCCTGCGGCAGAGACAAATGGACCGGCATGCCAAGATCAAGAGAGCCATCACCTTCATCA
TGGTGGTGGCCATCGTCTTTGTCATCTGCTTCCTTCCCAGCGTGGTTGTGCGGATCCGCATCTTCTGGCT
CCTGCACACTTCGGGCACGCAGAATTGTGAAGTGTACCGCTCGGTGGACCTGGCGTTCTTTATCACTCTC
AGCTTCACCTACATGAACAGCATGCTGGACCCCGTGGTGTACTACTTCTCCAGCCCATCCTTTCCCAACT
TCTTCTCCACTTTGATCAACCGCTGCCTCCAGAGGAAGATGACAGGTGAGCCAGATAATAACCGCAGCAC
GAGCGTCGAGCTCACAGGGGACCCCAACAAAACCAGAGGCGCTCCAGAGGCGTTAATGGCCAACTCCGGT
GAGCCATGGAGCCCCTCTTATCTGGGCCCAACCTCTCCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC206527 protein sequence
Red=Cloning site Green=Tags(s)

MNRHHLQDHFLEIDKKNCCVFRDDFIVKVLPPVLGLEFIFGLLGNGLALWIFCFHLKSWKSSRIFLFNLA
VADFLLIICLPFLMDNYVRRWDWKFGDIPCRLMLFMLAMNRQGSIIFLTVVAVDRYFRVVHPHHALNKIS
NRTAAIISCLLWGITIGLTVHLLKKKMPIQNGGANLCSSFSICHTFQWHEAMFLLEFFLPLGIILFCSAR
IIWSLRQRQMDRHAKIKRAITFIMVVAIVFVICFLPSVVVRIRIFWLLHTSGTQNCEVYRSVDLAFFITL
SFTYMNSMLDPVVYYFSSPSFPNFFSTLINRCLQRKMTGEPDNNRSTSVELTGDPNKTRGAPEALMANSG
EPWSPSYLGPTSP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_177551
ORF Size 1089 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177551.4
RefSeq Size 2082 bp
RefSeq ORF 1092 bp
Locus ID 338442
UniProt ID Q8TDS4
Cytogenetics 12q24.31
Protein Families Druggable Genome, GPCR, Transmembrane
MW 41.8 kDa
Summary Acts as a high affinity receptor for both nicotinic acid (also known as niacin) and (D)-beta-hydroxybutyrate and mediates increased adiponectin secretion and decreased lipolysis through G(i)-protein-mediated inhibition of adenylyl cyclase. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet. Mediates nicotinic acid-induced apoptosis in mature neutrophils. Receptor activation by nicotinic acid results in reduced cAMP levels which may affect activity of cAMP-dependent protein kinase A and phosphorylation of target proteins, leading to neutrophil apoptosis. The rank order of potency for the displacement of nicotinic acid binding is 5-methyl pyrazole-3-carboxylic acid = pyridine-3-acetic acid > acifran > 5-methyl nicotinic acid = acipimox >> nicotinuric acid = nicotinamide.[UniProtKB/Swiss-Prot Function]
Write Your Own Review
You're reviewing:HCAR2 (NM_177551) Human Tagged ORF Clone
Your Rating
SKU Description Size Price
RC206527L1 Lenti ORF clone of Human G protein-coupled receptor 109A (GPR109A), Myc-DDK-tagged 10 ug
$757.00
RC206527L2 Lenti ORF clone of Human G protein-coupled receptor 109A (GPR109A), mGFP tagged 10 ug
$757.00
RC206527L3 Lenti ORF clone of Human G protein-coupled receptor 109A (GPR109A), Myc-DDK-tagged 10 ug
$757.00
RC206527L4 Lenti ORF clone of Human G protein-coupled receptor 109A (GPR109A), mGFP tagged 10 ug
$757.00
RG206527 HCAR2 (tGFP-tagged) - Human G protein-coupled receptor 109A (GPR109A) 10 ug
$489.00 MSRP $657.00 MSRP $657.00
SC309249 HCAR2 (untagged)-Human G protein-coupled receptor 109A (GPR109A) 10 ug
$457.00

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.