PADI4 (NM_012387) Human Tagged ORF Clone

CAT#: RC206501

PADI4 (Myc-DDK-tagged)-Human peptidyl arginine deiminase, type IV (PADI4)

Product Images

Western blot validation of overexpression lysate (Cat# LY402202) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206501 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PADI4 protein (Cat# TP306501). The protein was produced from HEK293T cells transfected with PADI4 cDNA clone (Cat# RC206501) using MegaTran 2.0 (Cat# TT210002).

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PADI4 (Cat# RC206501, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PADI4(Cat# TA504813). Positive lysates LY402202 (100ug) and LC402202 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PADI4
• Synonyms: PAD; PAD4; PADI5; PDI4; PDI5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206501 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCAGGGGACATTGATCCGTGTGACCCCAGAGCAGCCCACCCATGCCGTGTGTGTGCTGGGCACCT
  TGACTCAGCTTGACATCTGCAGCTCTGCCCCTGAGGACTGCACGTCCTTCAGCATCAACGCCTCCCCAGG
  GGTGGTCGTGGATATTGCCCACAGCCCTCCAGCCAAGAAGAAATCCACAGGTTCCTCCACATGGCCCCTG
  GACCCTGGGGTAGAGGTGACCCTGACGATGAAAGCGGCCAGTGGTAGCACAGGCGACCAGAAGGTTCAGA
  TTTCATACTACGGACCCAAGACTCCACCAGTCAAAGCTCTACTCTACCTCACCGCGGTGGAAATCTCCCT
  GTGCGCAGACATCACCCGCACCGGCAAAGTGAAGCCAACCAGAGCTGTGAAAGATCAGAGGACCTGGACC
  TGGGGCCCTTGTGGACAGGGTGCCATCCTGCTGGTGAACTGTGACAGAGACAATCTCGAATCTTCTGCCA
  TGGACTGCGAGGATGATGAAGTGCTTGACAGCGAAGACCTGCAGGACATGTCGCTGATGACCCTGAGCAC
  GAAGACCCCCAAGGACTTCTTCACAAACCATACACTGGTGCTCCACGTGGCCAGGTCTGAGATGGACAAA
  GTGAGGGTGTTTCAGGCCACACGGGGCAAACTGTCCTCCAAGTGCAGCGTAGTCTTGGGTCCCAAGTGGC
  CCTCTCACTACCTGATGGTCCCCGGTGGAAAGCACAACATGGACTTCTACGTGGAGGCCCTCGCTTTCCC
  GGACACCGACTTCCCGGGGCTCATTACCCTCACCATCTCCCTGCTGGACACGTCCAACCTGGAGCTCCCC
  GAGGCTGTGGTGTTCCAAGACAGCGTGGTCTTCCGCGTGGCGCCCTGGATCATGACCCCCAACACCCAGC
  CCCCGCAGGAGGTGTACGCGTGCAGTATTTTTGAAAATGAGGACTTCCTGAAGTCAGTGACTACTCTGGC
  CATGAAAGCCAAGTGCAAGCTGACCATCTGCCCTGAGGAGGAGAACATGGATGACCAGTGGATGCAGGAT
  GAAATGGAGATCGGCTACATCCAAGCCCCACACAAAACACTGCCCGTGGTCTTCGACTCTCCAAGGAACA
  GAGGCCTGAAGGAGTTTCCCATCAAACGAGTGATGGGTCCAGATTTTGGCTATGTAACTCGAGGGCCCCA
  AACAGGGGGTATCAGTGGACTGGACTCCTTTGGGAACCTGGAAGTGAGCCCCCCAGTCACAGTCAGGGGC
  AAGGAATACCCGCTGGGCAGGATTCTCTTCGGGGACAGCTGTTATCCCAGCAATGACAGCCGGCAGATGC
  ACCAGGCCCTGCAGGACTTCCTCAGTGCCCAGCAGGTGCAGGCCCCTGTGAAGCTCTATTCTGACTGGCT
  GTCCGTGGGCCACGTGGACGAGTTCCTGAGCTTTGTGCCAGCACCCGACAGGAAGGGCTTCCGGCTGCTC
  CTGGCCAGCCCCAGGTCCTGCTACAAACTGTTCCAGGAGCAGCAGAATGAGGGCCACGGGGAGGCCCTGC
  TGTTCGAAGGGATCAAGAAAAAAAAACAGCAGAAAATAAAGAACATTCTGTCAAACAAGACATTGAGAGA
  ACATAATTCATTTGTGGAGAGATGCATCGACTGGAACCGCGAGCTGCTGAAGCGGGAGCTGGGCCTGGCC
  GAGAGTGACATCATTGACATCCCGCAGCTCTTCAAGCTCAAAGAGTTCTCTAAGGCGGAAGCTTTTTTCC
  CCAACATGGTGAACATGCTGGTGCTAGGGAAGCACCTGGGCATCCCCAAGCCCTTCGGGCCCGTCATCAA
  CGGCCGCTGCTGCCTGGAGGAGAAGGTGTGTTCCCTGCTGGAGCCACTGGGCCTCCAGTGCACCTTCATC
  AACGACTTCTTCACCTACCACATCAGGCATGGGGAGGTGCACTGCGGCACCAACGTGCGCAGAAAGCCCT
  TCTCCTTCAAGTGGTGGAACATGGTGCCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206501 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAQGTLIRVTPEQPTHAVCVLGTLTQLDICSSAPEDCTSFSINASPGVVVDIAHSPPAKKKSTGSSTWPL
  DPGVEVTLTMKAASGSTGDQKVQISYYGPKTPPVKALLYLTAVEISLCADITRTGKVKPTRAVKDQRTWT
  WGPCGQGAILLVNCDRDNLESSAMDCEDDEVLDSEDLQDMSLMTLSTKTPKDFFTNHTLVLHVARSEMDK
  VRVFQATRGKLSSKCSVVLGPKWPSHYLMVPGGKHNMDFYVEALAFPDTDFPGLITLTISLLDTSNLELP
  EAVVFQDSVVFRVAPWIMTPNTQPPQEVYACSIFENEDFLKSVTTLAMKAKCKLTICPEEENMDDQWMQD
  EMEIGYIQAPHKTLPVVFDSPRNRGLKEFPIKRVMGPDFGYVTRGPQTGGISGLDSFGNLEVSPPVTVRG
  KEYPLGRILFGDSCYPSNDSRQMHQALQDFLSAQQVQAPVKLYSDWLSVGHVDEFLSFVPAPDRKGFRLL
  LASPRSCYKLFQEQQNEGHGEALLFEGIKKKKQQKIKNILSNKTLREHNSFVERCIDWNRELLKRELGLA
  ESDIIDIPQLFKLKEFSKAEAFFPNMVNMLVLGKHLGIPKPFGPVINGRCCLEEKVCSLLEPLGLQCTFI
  NDFFTYHIRHGEVHCGTNVRRKPFSFKWWNMVP
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012387
• ORF Size: 1989 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012387.3
• RefSeq Size: 2265 bp
• RefSeq ORF: 1992 bp
• Locus ID: 23569
• UniProt ID: Q9UM07
• Cytogenetics: 1p36.13
• Domains: PAD
• MW: 74.1 kDa
• Gene Summary: This gene is a member of a gene family which encodes enzymes responsible for the conversion of arginine residues to citrulline residues. This gene may play a role in granulocyte and macrophage development leading to inflammation and immune response. [provided by RefSeq, Jul 2008]