RBM7 (NM_016090) Human Tagged ORF Clone

CAT#: RC206461

Reviews ()
Write a review

RBM7 (Myc-DDK-tagged)-Human RNA binding motif protein 7 (RBM7)



  "NM_016090" in other vectors (4)

Reconstitution Protocol

USD 300.00

In Stock*

Size
    • 10 ug

Product images

Frequently bought together (4)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 30 ul

USD 177.00


pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

USD 650.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 160.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00

Other products for "RBM7"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol RBM7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC206461 representing NM_016090
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGCGGCGGCGGCGGAAGCGGATCGCACTCTCTTTGTGGGCAACCTTGAAACGAAAGTGACCGAGG
AGCTCCTTTTCGAGCTTTTCCACCAGGCTGGGCCAGTAATAAAGGTGAAAATTCCAAAAGATAAGGATGG
TAAACCAAAGCAGTTTGCGTTTGTGAATTTCAAACATGAAGTGTCTGTTCCTTATGCAATGAATCTACTT
AATGGAATCAAACTTTATGGAAGGCCTATCAAAATTCAATTTAGATCAGGAAGTAGTCATGCCCCACAAG
ATGTCAGTTTGTCATATCCCCAACATCATGTTGGAAATTCAAGCCCTACCTCCACATCTCCTAGCAGGTA
CGAAAGGACTATGGATAACATGACTTCATCAGCACAGATAATTCAGAGATCTTTCTCTTCTCCAGAAAAT
TTTCAGAGACAAGCAGTGATGAACAGTGCTTTGAGACAAATGTCATATGGTGGAAAATTTGGTTCTTCAC
CTCTGGATCAATCAGGATTTTCACCATCAGTTCAATCACACAGTCATAGTTTCAATCAGTCTTCAAGCTC
CCAGTGGCGCCAAGGTACACCATCATCACAGCGTAAAGTCAGAATGAATTCTTATCCCTACCTAGCAGAT
AGACATTATAGCCGGGAACAGCGTTACACTGATCATGGGTCTGACCATCATTACAGAGGAAAGAGAGATG
ATTTCTTCTATGAAGACAGGAATCATGATGACTGGAGCCATGACTATGATAACAGAAGAGACAGTAGTAG
AGATGGAAAATGGCGCTCATCTCGACAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC206461 representing NM_016090
Red=Cloning site Green=Tags(s)

MGAAAAEADRTLFVGNLETKVTEELLFELFHQAGPVIKVKIPKDKDGKPKQFAFVNFKHEVSVPYAMNLL
NGIKLYGRPIKIQFRSGSSHAPQDVSLSYPQHHVGNSSPTSTSPSRYERTMDNMTSSAQIIQRSFSSPEN
FQRQAVMNSALRQMSYGGKFGSSPLDQSGFSPSVQSHSHSFNQSSSSQWRQGTPSSQRKVRMNSYPYLAD
RHYSREQRYTDHGSDHHYRGKRDDFFYEDRNHDDWSHDYDNRRDSSRDGKWRSSRH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_016090
ORF Size 798 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_016090.4
RefSeq Size 2025 bp
RefSeq ORF 801 bp
Locus ID 10179
UniProt ID Q9Y580
Cytogenetics 11q23.2
Domains RRM
MW 30.3 kDa
Gene Summary Subunit of the trimeric nuclear exosome targeting (NEXT) complex, a complex that directs a subset of non-coding short-lived RNAs for exosomal degradation. The RNA exosome is fundamental for the degradation of RNA in eukaryotic nuclei. Substrate targeting is facilitated by its cofactor MTREX, which links to RNA-binding protein adapters (PubMed:27871484). Possible involved in germ cell RNA processing and meiosis (Probable).[UniProtKB/Swiss-Prot Function]

Other Versions

Customer Reviews 
Loading...
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.