MX2 (NM_002463) Human Tagged ORF Clone

CAT#: RC206437

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MX2 (Myc-DDK-tagged)-Human myxovirus (influenza virus) resistance 2 (mouse) (MX2)



Reconstitution Protocol

USD 982.00

In Stock*

Size
    • 10 ug

Product images

Frequently bought together (4)
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pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol MX2
Synonyms MXB
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC206437 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTAAGGCCCACAAGCCTTGGCCCTACCGGAGGAGAAGTCAATTTTCTTCTCGAAAATACCTGAAAA
AAGAAATGAATTCCTTCCAGCAACAGCCACCGCCATTCGGCACAGTGCCACCACAAATGATGTTTCCTCC
AAACTGGCAGGGGGCAGAGAAGGACGCTGCTTTCCTCGCCAAGGACTTCAACTTTCTCACTTTGAACAAT
CAGCCACCACCAGGAAACAGGAGCCAACCAAGGGCAATGGGGCCCGAGAACAACCTGTACAGCCAGTACG
AGCAGAAGGTGCGCCCCTGCATTGACCTCATCGACTCCCTGCGGGCTCTGGGTGTGGAGCAGGACCTGGC
CCTGCCAGCCATCGCCGTCATCGGGGACCAGAGCTCGGGCAAGAGCTCTGTGCTGGAGGCACTGTCAGGA
GTCGCGCTTCCCAGAGGCAGCGGAATCGTAACCAGGTGTCCGCTGGTGCTGAAACTGAAAAAGCAGCCCT
GTGAGGCATGGGCCGGAAGGATCAGCTACCGGAACACCGAGCTAGAGCTTCAGGACCCTGGCCAGGTGGA
GAAAGAGATACACAAAGCCCAGAACGTCATGGCCGGGAATGGCCGGGGCATCAGCCATGAGCTCATCAGC
CTGGAGATCACCTCCCCTGAGGTTCCAGACCTGACCATCATTGACCTTCCCGGCATCACCAGGGTGGCTG
TGGACAACCAGCCCCGAGACATCGGACTGCAGATCAAGGCTCTCATCAAGAAGTACATCCAGAGGCAGCA
GACGATCAACTTGGTGGTGGTTCCCTGTAACGTGGACATTGCCACCACGGAGGCGCTGAGCATGGCCCAT
GAGGTGGACCCGGAAGGGGACAGGACCATCGGTATCCTGACCAAACCAGATCTAATGGACAGGGGCACTG
AGAAAAGCGTCATGAATGTGGTGCGGAACCTCACGTACCCCCTCAAGAAGGGCTACATGATTGTGAAGTG
CCGGGGCCAGCAGGAGATCACAAACAGGCTGAGCTTGGCAGAGGCAACCAAGAAAGAAATTACATTCTTT
CAAACACATCCATATTTCAGAGTTCTCCTGGAGGAGGGGTCAGCCACGGTTCCCCGACTGGCAGAAAGAC
TTACCACTGAACTCATCATGCATATCCAAAAATCGCTCCCGTTGTTAGAAGGACAAATAAGGGAGAGCCA
CCAGAAGGCGACCGAGGAGCTGCGGCGTTGCGGGGCTGACATCCCCAGCCAGGAGGCCGACAAGATGTTC
TTTCTAATTGAGAAAATCAAGATGTTTAATCAGGACATCGAAAAGTTAGTAGAAGGAGAAGAAGTTGTAA
GGGAGAATGAGACCCGTTTATACAACAAAATCAGAGAGGATTTTAAAAACTGGGTAGGCATACTTGCAAC
TAATACCCAAAAAGTTAAAAATATTATCCACGAAGAAGTTGAAAAATATGAAAAGCAGTATCGAGGCAAG
GAGCTTCTGGGATTTGTCAACTACAAGACATTTGAGATCATCGTGCATCAGTACATCCAGCAGCTGGTGG
AGCCCGCCCTTAGCATGCTCCAGAAAGCCATGGAAATTATCCAGCAAGCTTTCATTAACGTGGCCAAAAA
ACATTTTGGCGAATTTTTCAACCTTAACCAAACTGTTCAGAGCACGATTGAAGACATAAAAGTGAAACAC
ACAGCAAAGGCAGAAAACATGATCCAACTTCAGTTCAGAATGGAGCAGATGGTTTTTTGTCAAGATCAGA
TTTACAGTGTTGTTCTGAAGAAAGTCCGAGAAGAGATTTTTAACCCTCTGGGGACGCCTTCACAGAATAT
GAAGTTGAACTCTCATTTTCCCAGTAATGAGTCTTCGGTTTCCTCCTTTACTGAAATAGGCATCCACCTG
AATGCCTACTTCTTGGAAACCAGCAAACGTCTCGCCAACCAGATCCCATTTATAATTCAGTATTTTATGC
TCCGAGAGAATGGTGACTCCTTGCAGAAAGCCATGATGCAGATACTACAGGAAAAAAATCGCTATTCCTG
GCTGCTTCAAGAGCAGAGTGAGACCGCTACCAAGAGAAGAATCCTTAAGGAGAGAATTTACCGGCTCACT
CAGGCGCGACACGCACTCTGTCAATTCTCCAGCAAAGAGATCCAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC206437 protein sequence
Red=Cloning site Green=Tags(s)

MSKAHKPWPYRRRSQFSSRKYLKKEMNSFQQQPPPFGTVPPQMMFPPNWQGAEKDAAFLAKDFNFLTLNN
QPPPGNRSQPRAMGPENNLYSQYEQKVRPCIDLIDSLRALGVEQDLALPAIAVIGDQSSGKSSVLEALSG
VALPRGSGIVTRCPLVLKLKKQPCEAWAGRISYRNTELELQDPGQVEKEIHKAQNVMAGNGRGISHELIS
LEITSPEVPDLTIIDLPGITRVAVDNQPRDIGLQIKALIKKYIQRQQTINLVVVPCNVDIATTEALSMAH
EVDPEGDRTIGILTKPDLMDRGTEKSVMNVVRNLTYPLKKGYMIVKCRGQQEITNRLSLAEATKKEITFF
QTHPYFRVLLEEGSATVPRLAERLTTELIMHIQKSLPLLEGQIRESHQKATEELRRCGADIPSQEADKMF
FLIEKIKMFNQDIEKLVEGEEVVRENETRLYNKIREDFKNWVGILATNTQKVKNIIHEEVEKYEKQYRGK
ELLGFVNYKTFEIIVHQYIQQLVEPALSMLQKAMEIIQQAFINVAKKHFGEFFNLNQTVQSTIEDIKVKH
TAKAENMIQLQFRMEQMVFCQDQIYSVVLKKVREEIFNPLGTPSQNMKLNSHFPSNESSVSSFTEIGIHL
NAYFLETSKRLANQIPFIIQYFMLRENGDSLQKAMMQILQEKNRYSWLLQEQSETATKRRILKERIYRLT
QARHALCQFSSKEIH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_002463
ORF Size 2145 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002463.2
RefSeq Size 2961 bp
RefSeq ORF 2148 bp
Locus ID 4600
UniProt ID P20592
Cytogenetics 21q22.3
Domains dynamin_2, dynamin, GED
Protein Families Druggable Genome
MW 82.1 kDa
Gene Summary The protein encoded by this gene has a nuclear and a cytoplasmic form and is a member of both the dynamin family and the family of large GTPases. The nuclear form is localized in a granular pattern in the heterochromatin region beneath the nuclear envelope. A nuclear localization signal (NLS) is present at the amino terminal end of the nuclear form but is lacking in the cytoplasmic form due to use of an alternate translation start codon. This protein is upregulated by interferon-alpha but does not contain the antiviral activity of a similar myxovirus resistance protein 1. [provided by RefSeq, Jul 2008]
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