CPNE7 (NM_153636) Human Tagged ORF Clone

CAT#: RC206428

CPNE7 (Myc-DDK-tagged)-Human copine VII (CPNE7), transcript variant 1

Product Images

Circular map for RC206428

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  Western blot validation of overexpression lysate (Cat# LY406997) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206428 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CPNE7 protein (Cat# TP306428). The protein was produced from HEK293T cells transfected with CPNE7 cDNA clone (Cat# RC206428) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CPNE7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206428 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGCGGGCTCGGAGCGCGGGGCGGCGGCAACCCCCGGGGGTTTGCCCGCGCCCTGCGCCTCGAAGG
  TGGAGCTGCGGCTCAGCTGCCGGCACCTGCTGGACCGCGACCCGCTCACCAAGTCCGACCCCAGCGTGGC
  GTTGCTGCAGCAGGCGCAGGGCCAGTGGGTGCAGGTGGGCAGAACCGAGGTGGTCCGGAGCAGCCTGCAT
  CCCGTGTTCTCCAAGGTCTTCACGGTGGACTACTACTTCGAGGAGGTGCAGAGGCTGCGCTTTGAGGTGT
  ACGACACGCATGGGCCCAGCGGCTTCAGCTGTCAGGAGGACGATTTCCTGGGGGGCATGGAGTGCACCCT
  GGGGCAGATTGTGGCCCAGAAGAAGGTGACCCGCCCGCTGCTGCTCAAGTTTGGCAGGAACGCTGGCAAG
  TCCACCATCACGGTGATCGCCGAGGACATCTCGGGGAACAACGGCTACGTGGAGCTCTCCTTCCGGGCCA
  GGAAGCTGGACGACAAGGACCTCTTCAGCAAGTCCGACCCCTTCCTGGAGCTCTACAGGGTCAACGACGA
  CCAGGGCTTGCAGCTGGTGTACAGGACGGAGGTGGTGAAGAACAACCTGAACCCGGTGTGGGAGGCCTTC
  AAAGTCTCTCTGAGTTCCCTCTGCAGCTGCGAGGAGACAAGGCCTCTAAAGTGCCTGGTCTGGGATTACG
  ACTCTCGAGGAAAGCACGACTTCATCGGAGAATTCTCTACCACCTTCGAGGAGATGCAGAAGGCCTTTGA
  GGAGGGGCAGGCCCAGTGGGACTGTGTGAACCCCAAATACAAGCAGAAGAGACGCAGTTATAAGAACTCA
  GGAGTGGTCGTCCTGGCTGACCTCAAGTTCCACAGGGTGTACTCCTTCCTGGACTATATCATGGGCGGCT
  GCCAGATCCACTTCACCGTGGCCATTGACTTCACCGCCTCCAATGGAGACCCGCGGAACAGCTGCTCCCT
  GCACTACATCAACCCCTACCAGCCGAACGAGTACCTGAAGGCACTGGTGTCCGTGGGCGAGATCTGCCAG
  GACTATGACAGTGACAAGAGGTTTTCCGCTTTGGGGTTTGGAGCCCGGATCCCTCCCAAGTATGAGGTGT
  CCCATGACTTTGCCATCAATTTCAACCCTGAGGACGATGAGTGTGAAGGCATCCAGGGCGTGGTGGAGGC
  CTACCAGAACTGCCTGCCCAGGGTCCAGCTCTACGGCCCCACCAACGTGGCGCCCATCATCTCCAAGGTG
  GCACGCGTGGCGGCGGCCGAGGAGAGCACCGGGAAAGCCTCTCAATACTACATCCTGCTGATCCTGACGG
  ACGGCGTGGTGACCGACATGGCCGACACACGGGAGGCCATTGTGCGTGCCTCACGCCTGCCCATGTCCAT
  CATCATCGTGGGCGTGGGCAACGCCGACTTCACCGACATGCAGGTCCTGGACGGCGACGACGGCGTCCTG
  CGCTCCCCACGGGGTGAGCCCGCGCTCCGGGACATCGTACAGTTCGTGCCCTTCCGGGAGCTCAAGAACG
  CATCCCCTGCGGCGCTGGCCAAGTGCGTGCTGGCCGAGGTCCCGAAGCAGGTGGTGGAGTACTACAGCCA
  CAGAGGCCTGCCCCCGAGAAGCCTGGGCGTCCCTGCCGGAGAGGCCAGCCCAGGCTGCACACCG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206428 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSAGSERGAAATPGGLPAPCASKVELRLSCRHLLDRDPLTKSDPSVALLQQAQGQWVQVGRTEVVRSSLH
  PVFSKVFTVDYYFEEVQRLRFEVYDTHGPSGFSCQEDDFLGGMECTLGQIVAQKKVTRPLLLKFGRNAGK
  STITVIAEDISGNNGYVELSFRARKLDDKDLFSKSDPFLELYRVNDDQGLQLVYRTEVVKNNLNPVWEAF
  KVSLSSLCSCEETRPLKCLVWDYDSRGKHDFIGEFSTTFEEMQKAFEEGQAQWDCVNPKYKQKRRSYKNS
  GVVVLADLKFHRVYSFLDYIMGGCQIHFTVAIDFTASNGDPRNSCSLHYINPYQPNEYLKALVSVGEICQ
  DYDSDKRFSALGFGARIPPKYEVSHDFAINFNPEDDECEGIQGVVEAYQNCLPRVQLYGPTNVAPIISKV
  ARVAAAEESTGKASQYYILLILTDGVVTDMADTREAIVRASRLPMSIIIVGVGNADFTDMQVLDGDDGVL
  RSPRGEPALRDIVQFVPFRELKNASPAALAKCVLAEVPKQVVEYYSHRGLPPRSLGVPAGEASPGCTP
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153636
• ORF Size: 1674 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_153636.1, NP_705900.1
• RefSeq Size: 2449 bp
• RefSeq ORF: 1677 bp
• Locus ID: 27132
• UniProt ID: Q9UBL6
• Cytogenetics: 16q24.3
• MW: 61.9 kDa
• Gene Summary: This gene encodes a member of the copine family, which is composed of calcium-dependent membrane-binding proteins. The gene product contains two N-terminal C2 domains and one von Willebrand factor A domain. The encoded protein may be involved in membrane trafficking. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008]