C11orf82 (DDIAS) (NM_145018) Human Tagged ORF Clone

CAT#: RC206347

C11orf82 (Myc-DDK-tagged)-Human chromosome 11 open reading frame 82 (C11orf82)

Product Images

Circular map for RC206347

• 

  Western blot validation of overexpression lysate (Cat# LY408080) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206347 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C11orf82
• Synonyms: C11orf82; noxin
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206347 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAGAAGACGAAAATTTCTTCTAGCCTCAGTACTTGCTCTCCAGAATTCAAGTTTTATATATCCAT
  CATGTCAGAAGTGCTTCTCTAGGATAATCCTGGTCTCCAAAAGGTCTAATTGTCCAAAATGTGGCTCTAC
  TGGTGAATCTGGAAATGCCAATTACAGATACAAACTTTCCTTAAAAGTTGCAGAATCAAACAAATTGTTT
  GTTATTACTGTATTTGGAAGTTGCTTAGATACATTTTTTGGTCTTACTGCCACTGGTTTGCACAGGTACA
  TTCAGGATCCTAATAAAATTCCAGAAACACTGGACAATGATACAACTCAGAATCTATTAACTAAAGCAGT
  TGAAACTTGCTTTGTTGGACAAAGCTTTATTTTTGGAGTGACGAATTTTGAAAACCAACCTGGACAAGGT
  TCAGATGCCAGTAACTTCTTACAGCAATGCTCTGACCACAAAAGAAAAGCCAAAGCACTAGTGGCTTGCC
  AGATTGTTCTACCAGACCCAGGTATTGCAGGCTTTACTGTCATTGACTACTTCCATCAACTTTTGCAGAC
  TTTTAATTTCAGGAAACTTCAGTGTGACTCTCAGGCACCTAACAATCACTTACTTGCTTTAGATCACTCA
  AATAGTGATCTCAGCAGCACATATACTTCTGACAGCACTTCTGATTTTTTCAAGTCCTGCAGCAAGGATA
  CTTTTTCAAAATTCTGGCAGCCATCACTTGAATTCACTTGCATTGTTTCACAACTAACAGATAATGATGA
  TTTTTCAGCTTCAGAACAAAGTAAGGCCTTTGGTACTCTTCAGCAGAACAGAAAGTCCATCTCCATTGCA
  GAGGCCACTGGTTCCAGTAGCTGCCATGATCCCATTCAGGATTCATGGAGCCTTGTTTCATATATGGATA
  AAAAGAGTACAGCAGAAAAGTTGGGTAAAGAACTTGGCTTACAAGCTAAGGAGCTGAGTGCAGTTCACAG
  CAGTCATCATGAAATTGGAGTTAATGACTCTAATTTATTCTCTTTGGAAATGCGAGAGCCCCTTGAGTCA
  AGTAATACAAAATCCTTCCACAGTGCAGTGGAAATTAAAAATAGGTCCCAGCATGAGCTACCATGTTTTC
  AGCATCATGGTATAGATACCCCAACTAGCCTTCAGAAGAGATCTGCATGTTGTCCACCTTCGTTACTCAG
  ACTTGAAGAGACAGCCAGCAGTTCCCAGGATGGTGACCCTCAAATTTGGGATGATCTGCCATTCTCTGAA
  AGCCTGAACAAGTTTCTGGCAGTTCTTGAAAGTGAGATTGCTGTAACCCAGGCAGATGTCAGTAGTAGGA
  AACATCATGTAGATAATGACATTGATAAATTTCATGCAGACCACAGCAGTTTATCTGTGACTCCCCAGAG
  AACTACTGGAGCCCTGCATACACCACCTATAGCTTTAAGATCATCACAAGTAATAGTCAAAGCAAACTGT
  AGCAAAGATGACTTCCTTTTCAACTGTAAAGGAAATCTAAGTCCTAGTGTTGAAAAGGAGTCACAACCAG
  ATAACAAAGTAGAGGCTGTCTCTGTAAATCATAATGGAAGAGATATGTCAGAATATTTTTTACCGAATCC
  TTACCTGTCAGCTCTGTCTTCATCTTCAAAAGATTTAGAAACAATAGTTACTCTTAAGAAGACTATCAGA
  ATCTCACCACACAGGGAGAGTGACCATTCTAGTCTAAATAACAAATATTTGAATGGATGTGGAGAAATAT
  CAGTTTCAGAAATGAATGAAAAGTTGACAACTCTGTGTTATAGGAAGTATAATGATGTCTCTGATCTTTG
  CAAATTAGAAAATAAACAATATTGTAGGTGGTCCAAGAACCAAGATGACAGTTTTACAATTTGCAGGAAA
  CTTACATATCCTTTAGAAACTCTTTGCAATAGTCCAAATAGAAGTACAAATACATTGAAAGAAATGCCTT
  GGGGACATATCAATAACAACGTAACACAGAGCTATTCTATTGGTTATGAAGGTAGCTATGATGCCTCTGC
  TGATCTCTTTGATGATATTGCTAAAGAAATGGACATTGCAACTGAGATTACCAAAAAATCACAGGATATT
  TTGTTAAAATGGGGAACATCTTTGGCAGAAAGTCACCCTTCAGAGTCTGATTTTTCACTGAGATCACTTT
  CTGAAGACTTCATCCAGCCTTCACAAAAATTATCCTTGCAAAGCCTATCTGACTCTAGGCATTCAAGAAC
  ATGCTCTCCAACACCTCATTTTCAATCAGATTCAGAATATAATTTTGAAAATAGTCAAGACTTTGTTCCA
  TGTTCACAGTCAACTCCAATTTCAGGGTTCCACCAAACAAGAATTCATGGGATAAACAGAGCTTTCAAAA
  AACCTGTATTTTATTCAGATCTTGATGGTAACTATGAAAAAATAAGGATTTTCCCTGAAAATGACAAACA
  GCAAGCCAGCCCAAGCTGTCCAAAAAATATAAAAACACCTAGCCAGAAAATCAGAAGCCCTATTGTATCT
  GGTATTTCACAACCAGACGTTTTCAATCACTACCCTTTTGCTGAGTGCCATGAAACTGATAGTGATGAAT
  GGGTCCCTCCTACCACACAAAAAATATTTCCTTCAGATATGCTTGGATTCCAAGGCATAGGTCTAGGGAA
  ATGCCTTGCTGCCTATCATTTCCCTGATCAACAAGAGTTACCAAGAAAGAAACTGAAACATATTAGACAA
  GGAACCAATAAAGGTTTAATTAAGAAGAAATTAAAGAATATGCTTGCAGCAGTTGTTACGAAAAAGAAAA
  CTCATAAATATAACTGTAAAAGTTCAGGCTGGATTTCCAAATGTCCAGACATTCAAGTCTTAGCAGCACC
  TCAGCTGCACCCTATTCTTGGACCTGATTCTTGTTCAGAAGTCAAATGTTGCCTTCCATTTTCAGAAAAA
  GGCCCACCTTCAGTGTGTGAAACTCGAAGTGCTTGGTCACCTGAATTGTTTTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206347 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNRRRKFLLASVLALQNSSFIYPSCQKCFSRIILVSKRSNCPKCGSTGESGNANYRYKLSLKVAESNKLF
  VITVFGSCLDTFFGLTATGLHRYIQDPNKIPETLDNDTTQNLLTKAVETCFVGQSFIFGVTNFENQPGQG
  SDASNFLQQCSDHKRKAKALVACQIVLPDPGIAGFTVIDYFHQLLQTFNFRKLQCDSQAPNNHLLALDHS
  NSDLSSTYTSDSTSDFFKSCSKDTFSKFWQPSLEFTCIVSQLTDNDDFSASEQSKAFGTLQQNRKSISIA
  EATGSSSCHDPIQDSWSLVSYMDKKSTAEKLGKELGLQAKELSAVHSSHHEIGVNDSNLFSLEMREPLES
  SNTKSFHSAVEIKNRSQHELPCFQHHGIDTPTSLQKRSACCPPSLLRLEETASSSQDGDPQIWDDLPFSE
  SLNKFLAVLESEIAVTQADVSSRKHHVDNDIDKFHADHSSLSVTPQRTTGALHTPPIALRSSQVIVKANC
  SKDDFLFNCKGNLSPSVEKESQPDNKVEAVSVNHNGRDMSEYFLPNPYLSALSSSSKDLETIVTLKKTIR
  ISPHRESDHSSLNNKYLNGCGEISVSEMNEKLTTLCYRKYNDVSDLCKLENKQYCRWSKNQDDSFTICRK
  LTYPLETLCNSPNRSTNTLKEMPWGHINNNVTQSYSIGYEGSYDASADLFDDIAKEMDIATEITKKSQDI
  LLKWGTSLAESHPSESDFSLRSLSEDFIQPSQKLSLQSLSDSRHSRTCSPTPHFQSDSEYNFENSQDFVP
  CSQSTPISGFHQTRIHGINRAFKKPVFYSDLDGNYEKIRIFPENDKQQASPSCPKNIKTPSQKIRSPIVS
  GISQPDVFNHYPFAECHETDSDEWVPPTTQKIFPSDMLGFQGIGLGKCLAAYHFPDQQELPRKKLKHIRQ
  GTNKGLIKKKLKNMLAAVVTKKKTHKYNCKSSGWISKCPDIQVLAAPQLHPILGPDSCSEVKCCLPFSEK
  GPPSVCETRSAWSPELFS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145018
• ORF Size: 2994 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145018.2, NP_659455.2
• RefSeq Size: 3577 bp
• RefSeq ORF: 2997 bp
• Locus ID: 220042
• UniProt ID: Q8IXT1
• Cytogenetics: 11q14.1
• MW: 111.5 kDa
• Gene Summary: May be an anti-apoptotic protein involved in DNA repair or cell survival.[UniProtKB/Swiss-Prot Function]