alpha 2 Glycine Receptor (GLRA2) (NM_002063) Human Tagged ORF Clone

CAT#: RC206298

GLRA2 (Myc-DDK-tagged)-Human glycine receptor, alpha 2 (GLRA2), transcript variant 1

Product Images

Circular map for RC206298

• 

  Western blot validation of overexpression lysate (Cat# LY426528) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC225747 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: alpha 2 Glycine Receptor
• Synonyms: GLR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206298 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCGGCAGCTAGTGAACATTTTGACAGCCTTGTTTGCATTTTTCTTAGAGACAAACCACTTCAGGA
  CGGCTTTCTGCAAAGACCATGACTCCAGGTCTGGAAAACAACCTTCACAGACCCTATCTCCTTCAGATTT
  CTTGGACAAGTTAATGGGAAGGACATCAGGATATGATGCAAGAATCAGGCCAAATTTTAAAGGTCCTCCA
  GTAAACGTTACTTGCAATATTTTTATCAACAGTTTTGGATCAGTCACAGAAACGACCATGGACTACCGAG
  TGAATATTTTTCTGAGACAACAGTGGAATGATTCACGGCTGGCGTACAGTGAGTACCCAGATGACTCCCT
  GGACTTGGACCCATCCATGCTAGACTCCATTTGGAAACCAGATTTGTTCTTTGCCAATGAGAAGGGTGCC
  AACTTCCACGATGTCACCACTGACAACAAATTGCTACGGATTTCGAAAAATGGCAAAGTGCTCTACAGTA
  TCAGACTCACCTTGACCTTATCCTGTCCCATGGACTTGAAGAACTTTCCGATGGATGTCCAGACCTGTAC
  AATGCAGCTGGAGAGTTTTGGGTACACGATGAATGACCTGATATTTGAGTGGTTAAGTGATGGTCCAGTG
  CAAGTTGCTGAAGGATTGACCCTGCCCCAGTTTATTTTGAAAGAAGAGAAGGAACTTGGCTACTGTACAA
  AGCACTACAACACTGGAAAGTTTACCTGCATTGAGGTCAAGTTTCATCTGGAACGCCAAATGGGATATTA
  TTTGATCCAGATGTACATCCCAAGCCTGCTTATAGTAATTTTGTCCTGGGTTTCCTTTTGGATAAATATG
  GATGCAGCCCCTGCCAGGGTCGCACTGGGCATCACCACAGTCTTAACGATGACCACCCAGAGTTCAGGCT
  CCAGGGCATCTCTGCCAAAGGTCTCCTATGTAAAAGCGATTGACATCTGGATGGCGGTGTGCCTTCTGTT
  TGTGTTTGCTGCCTTACTGGAATACGCAGCGGTGAACTTCGTCTCCAGGCAACACAAGGAGTTCCTGCGC
  CTCCGAAGAAGACAGAAGAGGCAGAATAAGGAAGAAGACGTTACTCGTGAAAGTCGTTTTAATTTTAGCG
  GTTATGGGATGGGTCACTGCCTCCAAGTGAAAGATGGAACAGCTGTCAAGGCCACACCTGCCAACCCACT
  CCCACAACCGCCAAAAGATGGAGATGCTATCAAGAAGAAGTTTGTGGACCGGGCAAAAAGGATTGACACG
  ATATCTCGAGCTGCCTTCCCATTGGCCTTCCTCATTTTCAACATCTTTTACTGGATCACATACAAGATCA
  TTCGGCATGAAGATGTCCACAAGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206298 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNRQLVNILTALFAFFLETNHFRTAFCKDHDSRSGKQPSQTLSPSDFLDKLMGRTSGYDARIRPNFKGPP
  VNVTCNIFINSFGSVTETTMDYRVNIFLRQQWNDSRLAYSEYPDDSLDLDPSMLDSIWKPDLFFANEKGA
  NFHDVTTDNKLLRISKNGKVLYSIRLTLTLSCPMDLKNFPMDVQTCTMQLESFGYTMNDLIFEWLSDGPV
  QVAEGLTLPQFILKEEKELGYCTKHYNTGKFTCIEVKFHLERQMGYYLIQMYIPSLLIVILSWVSFWINM
  DAAPARVALGITTVLTMTTQSSGSRASLPKVSYVKAIDIWMAVCLLFVFAALLEYAAVNFVSRQHKEFLR
  LRRRQKRQNKEEDVTRESRFNFSGYGMGHCLQVKDGTAVKATPANPLPQPPKDGDAIKKKFVDRAKRIDT
  ISRAAFPLAFLIFNIFYWITYKIIRHEDVHKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002063
• ORF Size: 1356 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002063.4
• RefSeq Size: 3458 bp
• RefSeq ORF: 1359 bp
• Locus ID: 2742
• UniProt ID: P23416
• Cytogenetics: Xp22.2
• Domains: Neur_chan_memb, Neur_chan_LBD
• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 52 kDa
• Gene Summary: The glycine receptor consists of two subunits, alpha and beta, and acts as a pentamer. The protein encoded by this gene is an alpha subunit and can bind strychnine. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2010]