TMEM106B (NM_018374) Human Tagged ORF Clone

CAT#: RC206257

TMEM106B (Myc-DDK-tagged)-Human transmembrane protein 106B (TMEM106B), transcript variant 1

Product Images

Circular map for RC206257

• 

  Western blot validation of overexpression lysate (Cat# LY427384) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC225374 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TMEM106B
• Synonyms: HLD16
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206257 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAAAGTCTCTTTCTCATTTGCCTTTGCATTCAAGCAAAGAAGATGCTTATGATGGAGTCACATCTG
  AAAACATGAGGAATGGACTGGTTAATAGTGAAGTCCATAATGAAGATGGAAGAAATGGAGATGTCTCTCA
  GTTTCCATATGTGGAATTTACAGGAAGAGATAGTGTCACCTGCCCTACTTGTCAGGGAACAGGAAGAATT
  CCTAGGGGGCAAGAAAACCAACTGGTGGCATTGATTCCATATAGTGATCAGAGATTAAGGCCAAGAAGAA
  CAAAGCTGTATGTGATGGCTTCTGTGTTTGTCTGTCTACTCCTTTCTGGATTGGCTGTGTTTTTCCTTTT
  CCCTCGCTCTATCGACGTGAAATACATTGGTGTAAAATCAGCCTATGTCAGTTATGATGTTCAGAAGCGT
  ACAATTTATTTAAATATCACAAACACACTAAATATAACAAACAATAACTATTACTCTGTCGAAGTTGAAA
  ACATCACTGCCCAAGTTCAATTTTCAAAAACAGTTATTGGAAAGGCACGCTTAAACAACATAACCATTAT
  TGGTCCACTTGATATGAAACAAATTGATTACACAGTACCTACCGTTATAGCAGAGGAAATGAGTTATATG
  TATGATTTCTGTACTCTGATATCCATCAAAGTGCATAACATAGTACTCATGATGCAAGTTACTGTGACAA
  CAACATACTTTGGCCACTCTGAACAGATATCCCAGGAGAGGTATCAGTATGTCGACTGTGGAAGAAACAC
  AACTTATCAGTTGGGGCAGTCTGAATATTTAAATGTACTTCAGCCACAACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206257 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGKSLSHLPLHSSKEDAYDGVTSENMRNGLVNSEVHNEDGRNGDVSQFPYVEFTGRDSVTCPTCQGTGRI
  PRGQENQLVALIPYSDQRLRPRRTKLYVMASVFVCLLLSGLAVFFLFPRSIDVKYIGVKSAYVSYDVQKR
  TIYLNITNTLNITNNNYYSVEVENITAQVQFSKTVIGKARLNNITIIGPLDMKQIDYTVPTVIAEEMSYM
  YDFCTLISIKVHNIVLMMQVTVTTTYFGHSEQISQERYQYVDCGRNTTYQLGQSEYLNVLQPQQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018374
• ORF Size: 822 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018374.4
• RefSeq Size: 6514 bp
• RefSeq ORF: 825 bp
• Locus ID: 54664
• UniProt ID: Q9NUM4
• Cytogenetics: 7p21.3
• Protein Families: Transmembrane
• MW: 31.1 kDa
• Gene Summary: Involved in dendrite morphogenesis and maintenance by regulating lysosomal trafficking via its interaction with MAP6. May act by inhibiting retrograde transport of lysosomes along dendrites. Required for dendrite branching.[UniProtKB/Swiss-Prot Function]