EIF5A2 (NM_020390) Human Tagged ORF Clone
EIF5A2 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 5A2 (EIF5A2)
|Type||Human Tagged ORF Clone|
|E. coli Selection||Kanamycin (25 ug/mL)|
|Mammalian Cell Selection||Neomycin|
>RC206249 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC206249 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||459 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at firstname.lastname@example.org or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||5537 bp|
|RefSeq ORF||462 bp|
|Gene Summary||mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. Functions as a regulator of apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation (By similarity).[UniProtKB/Swiss-Prot Function]|
|cDNA Clone Resources|
|RC206249L1||Lenti ORF clone of Human eukaryotic translation initiation factor 5A2 (EIF5A2), Myc-DDK-tagged||
|RC206249L2||Lenti ORF clone of Human eukaryotic translation initiation factor 5A2 (EIF5A2), mGFP tagged||
|RC206249L3||Lenti ORF clone of Human eukaryotic translation initiation factor 5A2 (EIF5A2), Myc-DDK-tagged||
|RC206249L4||Lenti ORF clone of Human eukaryotic translation initiation factor 5A2 (EIF5A2), mGFP tagged||
|RG206249||EIF5A2 (GFP-tagged) - Human eukaryotic translation initiation factor 5A2 (EIF5A2)||
|SC126117||EIF5A2 (untagged)-Human eukaryotic translation initiation factor 5A2 (EIF5A2)||