GLIS3 (NM_152629) Human Tagged Lenti ORF Clone
CAT#: RC206232L3
- LentiORF®
Lenti ORF clone of Human GLIS family zinc finger 3 (GLIS3), transcript variant 2, Myc-DDK-tagged
"NM_152629" in other vectors (4)
Product Images
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | GLIS3 |
Synonyms | NDH; ZNF515 |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC206232).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_152629 |
ORF Size | 2325 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_152629.3, NP_689842.2, NP_689842.3 |
RefSeq Size | 6672 bp |
RefSeq ORF | 2328 bp |
Locus ID | 169792 |
UniProt ID | Q8NEA6 |
Cytogenetics | 9p24.2 |
MW | 83.5 kDa |
Gene Summary | This gene is a member of the GLI-similar zinc finger protein family and encodes a nuclear protein with five C2H2-type zinc finger domains. This protein functions as both a repressor and activator of transcription and is specifically involved in the development of pancreatic beta cells, the thyroid, eye, liver and kidney. Mutations in this gene have been associated with neonatal diabetes and congenital hypothyroidism (NDH). Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only two have been determined. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC206232 | GLIS3 (Myc-DDK-tagged)-Human GLIS family zinc finger 3 (GLIS3), transcript variant 2 |
USD 710.00 |
|
RC206232L4 | Lenti ORF clone of Human GLIS family zinc finger 3 (GLIS3), transcript variant 2, mGFP tagged |
USD 1,010.00 |
|
RG206232 | GLIS3 (tGFP-tagged) - Human GLIS family zinc finger 3 (GLIS3), transcript variant 2 |
USD 910.00 |
|
SC306467 | GLIS3 (untagged)-Human GLIS family zinc finger 3 (GLIS3), transcript variant 2 |
USD 711.00 |
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