NISCH (NM_007184) Human Tagged Lenti ORF Clone

CAT#: RC206026L1

  • LentiORF®

Lenti ORF clone of Human nischarin (NISCH), Myc-DDK-tagged


  "NM_007184" in other vectors (6)

Reconstitution Protocol

USD 1,586.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (5)
Lenti-vpak packaging kit - packaging plasmids and transfection reagent
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Forward primer to sequence targets cloned in expression vectors.
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Reverse primer to sequence targets cloned in expression vectors.
    • 1 nmol

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NISCH Antibody - C-terminal region
    • 100 ul

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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
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Other products for "NISCH"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol NISCH
Synonyms hIRAS; I-1; IR1; IRAS
Vector pLenti-C-Myc-DDK
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection None
Sequence Data
The ORF insert of this clone is exactly the same as(RC206026).
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NM_007184
ORF Size 4512 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_007184.2
RefSeq Size 5252 bp
RefSeq ORF 4515 bp
Locus ID 11188
UniProt ID Q9Y2I1
Cytogenetics 3p21.1
Domains LRR, PX, LRR_SD22
Protein Families Druggable Genome
MW 166.7 kDa
Gene Summary This gene encodes a nonadrenergic imidazoline-1 receptor protein that localizes to the cytosol and anchors to the inner layer of the plasma membrane. The orthologous mouse protein has been shown to influence cytoskeletal organization and cell migration by binding to alpha-5-beta-1 integrin. In humans, this protein has been shown to bind to the adapter insulin receptor substrate 4 (IRS4) to mediate translocation of alpha-5 integrin from the cell membrane to endosomes. Expression of this protein was reduced in human breast cancers while its overexpression reduced tumor growth and metastasis; possibly by limiting the expression of alpha-5 integrin. In human cardiac tissue, this gene was found to affect cell growth and death while in neural tissue it affected neuronal growth and differentiation. Alternative splicing results in multiple transcript variants encoding differerent isoforms. Some isoforms lack the expected C-terminal domains of a functional imidazoline receptor. [provided by RefSeq, Jan 2013]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.